Identification of GPCR binding proteins by using a novel anti-FLAG antibody and magnetic nanobeads
Project/Area Number |
23659157
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
General medical chemistry
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Research Institution | Juntendo University (2012) Kyushu University (2011) |
Principal Investigator |
|
Project Period (FY) |
2010 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
Keywords | GPCR / 免疫沈降 / 結合タンパク質 |
Research Abstract |
I established a novel anti-FLAG monoclonal antibody (2H8) with high-affinify and a low background. Combination of 2H8 antibody with a magnetic nanobeads enabled us to immunoprecipitate FLAG-tagged G-protein coupled receptors (GPCRs). Immunoprecipitated GPCRs were detectable by silver staining after separation by SDS-Page. I am analyzing some putative GPCR-binding proteins isolated by this novel immunoprecipitation techniques.
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Report
(3 results)
Research Products
(20 results)