microRNA analysis as the predictive tool of the breast cancer treatment

• Project/Area Number: 23659183
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Human pathology
• Research Institution: Tohoku University
• Principal Investigator: SASANO Hironobu 東北大学, 大学院・医学系研究科, 教授 (50187142)
• Co-Investigator(Renkei-kenkyūsha): ISHIDA Takanori 東北大学, 大学院・医学系研究科, 准教授 (00292318) ; SUZUKI Takashi 東北大学, 大学院・医学系研究科, 教授 (10261629) ; MIKI Yasuhiro 東北大学, 災害科学国際研究所, 講師 (50451521) ; ONO Katsuhiko 東北大学, 大学院・医学系研究科, 技術専門 職員 (80466531)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 乳癌 / microRNA / LIN28 / Ki-67 / HER2

Research Abstract

A new molecular technique as a breast cancer diagnosis is attracting a great deal of attention. In this research, I clarified relation between miRNA expression and pathological biomarkers using breast cancer tissues. These main results of this research are as follows: 1) LIN28 correlated with let-7 family 2) Identification of miRNAs relatedwith Ki-67, which is proliferation marker. and 3) Identification of miRNAs related with HER2, which is targeting molecule for breast cancer therapy. The results from our research suggest the possibility of the miRNA analysis as an novel diagnosis tool for breast cancer.

Research Products

• [Journal Article] LMTK3 is implicated in endocrine resistance via multiple signaling pathways (2013)
  • Author(s): Stebbing J, Filipovic A, Lit LC, Blighe K, Grothey A, Xu Y, Miki Y, Chow LW, Coombes RC, Sasano H, Shaw JA, Giamas G
  • Journal Title: Oncogene Volume: (In press) Issue: 28 Pages: 3371-3380
  • DOI: 10.1038/onc.2012.343
  • URL: http://www.nature.com/onc/journal/vaop/ncurrent/pdf/onc2012343a.pdf
  • Peer Reviewed
• [Journal Article] Androgenic pathway in triple negative invasive ductal tumors : Its correlation with tumor cell proliferation. (2013)
  • Author(s): McNamara KM (18人中1人目)
  • Journal Title: Cancer Sci. Volume: 104(5) Issue: 5 Pages: 639-646
  • DOI: 10.1111/cas.12121
  • Peer Reviewed
• [Journal Article] HIF-1alpha stimulates aromatase expression driven by prostaglandin E2 in breast adipose stroma (2013)
  • Author(s): Samarajeewa NU, Yang F, Docanto MM, Sakurai M, McNamara KM, Sasano H, Fox SB, Simpson ER, Brown K
  • Journal Title: Breast Cancer Res Volume: 15(2)
  • Peer Reviewed
• [Journal Article] Androgen receptor in triple negative breast cancer. (2013)
  • Author(s): McNamara KM (6人中1人目)
  • Journal Title: J Steroid Biochem Mol Biol Volume: 133 Pages: 66-76
  • DOI: 10.1016/j.jsbmb.2012.08.007
  • Peer Reviewed
• [Journal Article] Immunohistochemical analysis of aromatase in metastatic lymph nodes of breast cancer (2013)
  • Author(s): Shibahara Y, Miki Y, Ishida T, Nakamura Y, Suzuki T, Ohuchi N, Sasano H
  • Journal Title: Pathol Int Volume: 63(1) Issue: 1 Pages: 20-28
  • DOI: 10.1111/pin.12015
  • Peer Reviewed
• [Journal Article] Changes of tumor infiltrating lymphocyte subtypes before and after neoadjuvant endocrine therapy in estrogen receptor-positive breast cancer patients-a immunohistochemical study of Cd8+ and Foxp3+ using double immunostaining with correlation to the pathobiological response of the patients (2012)
  • Author(s): Chan MS, Wang L, Felizola SJ, Ueno T, Toi M, Loo W, Chow LW, Suzuki T, Sasano H
  • Journal Title: Int J Biol Markers Volume: 27(4) Issue: 4 Pages: 295-304
  • DOI: 10.5301/jbm.2012.10439
  • Peer Reviewed
• [Journal Article] An induction of microRNA, miR-7 through estrogen treatment in breast carcinoma (2012)
  • Author(s): Masuda M, Miki Y, Hata S, Takagi K, Sakurai M, Ono K, Suzuki K, Yang Y, Abe E, Hirakawa H, Ishida T, Suzuki T, Ohuchi N, Sasano H
  • Journal Title: J Transl Med Volume: 10
  • Peer Reviewed
• [Journal Article] Aromatase inhibitor treatment of breast cancer cells increases the expression of let-7f, a microRNA targeting CYP19A1 (2012)
  • Author(s): Shibahara Y, Miki Y, Onodera Y, Hata S, Chan MS, Yiu CC, Loo TY, Nakamura Y, Akahira J, Ishida T, Abe K, Hirakawa H, Chow LW, Suzuki T, Ouchi N, Sasano H
  • Journal Title: J Pathol Volume: 227 (3) Issue: 3 Pages: 357-66
  • DOI: 10.1002/path.4019
  • Peer Reviewed
• [Journal Article] Oestrogen-induced genes in ductal carcinoma in situ: their comparison with invasive ductal carcinoma (2012)
  • Author(s): Ebata A, Suzuki T, Takagi K, Miki Y, Onodera Y, Nakamura Y, Fujishima F, Ishida K, Watanabe M, Tamaki K, Ishida T, Ohuchi N, Sasano H
  • Journal Title: Endocr Relat Cancer Volume: 19 (4) Issue: 4 Pages: 485-96
  • DOI: 10.1530/erc-11-0345
  • Peer Reviewed
• [Journal Article] Analysis of clinically relevant values of Ki-67 labeling index in Japanese breast cancer patients. (2012)
  • Author(s): Tamaki K
  • Journal Title: Breast Cancer Volume: - Issue: 3 Pages: 325-333
  • DOI: 10.1007/s12282-012-0387-5
  • URL: http://link.springer.com/content/pdf/10.1007%2Fs12282-012-0387-5.pdf
  • Peer Reviewed
• [Journal Article] An induction of microRNA, miR-7 through estrogen treatment in breast carcinoma. (2012)
  • Author(s): Masuda M, Miki Y, Hata S, Takagi K, Sakurai M, Ono K, Suzuki K, Yang Y, Abe E, Hirakawa H, Ishida T, Suzuki T, Ohuchi N, Sasano H.
  • Journal Title: J Transl Med. Volume: Sep 19;10 Suppl 1 Issue: S1
  • DOI: 10.1186/1479-5876-10-s1-s2
  • Peer Reviewed
• [Journal Article] Epigenetic mechanisms regulate the prostaglandin E receptor 2 in breast cancer. (2012)
  • Author(s): To SQ, Takagi K, Miki Y, Suzuki K, Abe E, Yang Y, Sasano H, Simpson ER, Knower KC, Clyne CD.
  • Journal Title: J Steroid Biochem Mol Biol. Volume: Nov;132(3-5) Issue: 3-5 Pages: 331-338
  • DOI: 10.1016/j.jsbmb.2012.07.007
  • Peer Reviewed
• [Journal Article] LIN28: a regulator of tumor-suppressing activity of let-7 microRNA in human breast cancer. (2012)
  • Author(s): Sakurai M, Miki Y, Masuda M, Hata S, Shibahara Y, Hirakawa H, Suzuki T, Sasano H.
  • Journal Title: J Steroid Biochem Mol Biol. Volume: Sep;131(3-5) Issue: 3-5 Pages: 101-106
  • DOI: 10.1016/j.jsbmb.2011.10.007
  • Peer Reviewed
• [Journal Article] LIN28: A regulator of tumor-suppressing activity of let-7 microRNA in human breast cancer. (2012)
  • Author(s): Sakurai M, Miki Y, Masuda M, Hata S, Shibahara Y, Hirakawa H, Suzuki T, Sasano
  • Journal Title: J Steroid Biochem Mol Biol. Volume: 印刷中
  • Peer Reviewed
• [Presentation] LIN28B as a Potential Prognostic Marker of Breast Cancer Patients (2012)
  • Author(s): Minako SAKURAI, Yasuhiro MIKI, Yukiko SHIBAHARA, Hisashi HIRAKAWA, Noriaki Ouchi, Takashi SUZUKI, Hironobu SASANO
  • Organizer: 15th International Congress on Hormonal Steroids and Hormones & Cancer
  • Place of Presentation: 金沢市
  • Year and Date: 2012-11-17
• [Presentation] Aromatase inhibitor treatment of breast cancer cells increases the expression of let-7f, a microRNA targeting CYP19A1 (2012)
  • Author(s): Yukiko SHIBAHARA, Yasuhiro MIKI, Shuko HATA, Yoshiaki ONODERA, Noriaki OHUCHI, Hironobu SASANO
  • Organizer: 第71回日本 癌学会学術総会
  • Place of Presentation: 札幌市
  • Year and Date: 2012-09-20
• [Presentation] Methylation and expression of the prostanoid receptor EP2 in ER+ breast cancer (2012)
  • Author(s): Sarah Q TO, Kiyoshi TAKAGI, Yasuhiro MIKI, Koyu SUZUKI, Eriko ABE, Yang YANG, Hironobu SASANO, Evan R SIMPSON, Colin D CLYNE, Kevin C KNOWER
  • Organizer: 第71回日本癌 学会学術総会
  • Place of Presentation: 札幌市
  • Year and Date: 2012-09-19
• [Presentation] 乳癌におけるアロマターゼ阻害剤投 与による microRNA の変動および Let-7f による CYP19A1 の制御に関する検討 (2012)
  • Author(s): 柴原裕紀子、三木康宏、端秀子、小野寺好 明、中村保宏、赤平純一、阿部佳子、笹野 公伸
  • Organizer: 第101回日本病理学会総会
  • Place of Presentation: 東京都
  • Year and Date: 2012-04-26
• [Presentation] Aromatase inhibitor increases let-7f which targets CYP19A1 in Human Breast Cancer (2011)
  • Author(s): Yukiko SHIBAHARA, Yasuhiro MIKI, Yoshiaki ONODERA, Shuko HATA, Hironobu SASANO
  • Organizer: Global breast cancer conference 2011
  • Place of Presentation: Seoul (Korea)
  • Year and Date: 2011-10-06
• [Presentation] LIN28 regulates tumor suppressing activity of let-7 miRNA in human breast cancer (2011)
  • Author(s): Minako SAKURAI, Yasuhiro MIKI, Mariko MASUDA, Hisashi HIRAKAWA, Takashi SUZUKI, Hironobu SASANO
  • Organizer: 第70回日本癌学会 学術総会
  • Place of Presentation: 名古屋市
  • Year and Date: 2011-10-03
• [Presentation] 非浸潤性及び浸潤性乳癌組織におけるエストロゲン作用 (2011)
  • Author(s): 江幡明子、鈴木貴、高木清司、三木康宏、五十嵐やよい、中村保宏、石田和之、渡辺みか、石田孝宣、大内憲明、笹野公伸
  • Organizer: 第19回日本乳癌学会学術総会
  • Place of Presentation: 仙台市
  • Year and Date: 2011-09-02
• [Presentation] ヒト乳癌における Suppressor of Cytokine Signalling 3(SOCS3)発現の意義 (2011)
  • Author(s): 櫻井美奈子、三木康宏、石田孝宣、鈴木 貴、大内憲明、笹野公伸
  • Organizer: 第19回日本乳癌学 会学術総会
  • Place of Presentation: 仙台市
  • Year and Date: 2011-09-02
• [Presentation] ヒト ER 陽性乳癌におけるエストロゲン誘導性 microRNA-7 と EGFR 発 現 (2011)
  • Author(s): 増田真理子、三木康宏(発表)、端 秀子、鈴木 貴、石田孝宣、平川 久、大内憲明、笹野公伸
  • Organizer: 第19回日本乳癌学会学術総会
  • Place of Presentation: 仙台市
  • Year and Date: 2011-09-02
• [Presentation] 乳癌原発 巣および転移リンパ節でのアロマターゼ免 疫染色の比較検討 (2011)
  • Author(s): 柴原裕紀子、三木康宏、工藤陽花子、小野克彦、石田孝宣、笹野公伸
  • Organizer: 第19回日本乳癌学会学 術総会
  • Place of Presentation: 仙台市
  • Year and Date: 2011-09-02
• [Presentation] ヒト乳癌におけるLIN28によるlet-7発現抑制 (2011)
  • Author(s): 櫻井美奈子、三木康宏、増田真理子、柴原裕紀子、平川久、鈴木貴、笹野公伸
  • Organizer: 第12回ホルモンと癌研究会
  • Place of Presentation: 東京都
  • Year and Date: 2011-07-15
• [Presentation] 乳癌における Suppressor of Cytokine Signaling (SOCS3) の発現意義 (2011)
  • Author(s): 櫻井美奈子、三木康宏、石田孝宣、鈴木 貴、大内憲明、笹野公伸
  • Organizer: 第29回内分泌代謝学 サマーセミナー
  • Place of Presentation: 仙台市
  • Year and Date: 2011-07-08
• [Presentation] エストロゲン陽性乳癌における原発巣と転移リンパ節のアロマターゼ比較検討 (2011)
  • Author(s): 柴原裕紀子、三木康宏(発表)、小野克彦、工藤陽花子、中村保宏、笹野公伸
  • Organizer: 第29回内分泌代謝学サマーセミナー
  • Place of Presentation: 仙台市
  • Year and Date: 2011-07-08
• [Presentation] 乳癌における原発巣および転移リンパ節のアロマターゼ免疫染色の比較検討 (2011)
  • Author(s): 柴原裕紀子,三木康宏,阿部佳子,笹野公伸
  • Organizer: 第100回日本病理学会総会
  • Place of Presentation: 横浜市
  • Year and Date: 2011-04-28
• [Presentation] 乳癌組織におけるaromatase 発現調節機構の解明 (2011)
  • Author(s): 三木康宏
  • Organizer: 第19回日本乳癌学会学術総会(招待講演)
  • Place of Presentation: 仙台市
• [Presentation] ヒトER 陽性乳癌におけるエストロゲン誘導性microRNA-7 とEGFR 発現 (2011)
  • Author(s): 増田真理子、三木康宏、端 秀子、鈴木 貴、石田孝宣、平川 久、大内憲明、笹野公伸
  • Organizer: 第19回日本乳癌学会学術総会
  • Place of Presentation: 仙台市
• [Presentation] ヒト乳癌におけるLIN28によるlet-7発現制御 (2011)
  • Author(s): Minako SAKURAI, Yasuhiro MIKI, Mariko MASUDA, Hisashi HIRAKAWA, Takashi SUZUKI, Hironobu SASANO
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋市
• [Presentation] Aromatase inhibitor increases let-7f which targets CYP19A1 in Human Breast Cancer (2011)
  • Author(s): Yukiko SHIBAHARA, Yasuhiro MIKI, Yoshiaki ONODERA, Shuko HATA, Hironobu SASANO
  • Organizer: Global breast cancer conference
  • Place of Presentation: Seoul (Korea)
• [Presentation] Aromatase inhibitor treatment of breast cancer cells increases the expression of let-7f, a microRNA targeting CYP19A1
  • Author(s): Yukiko SHIBAHARA, Yasuhiro MIKI, Shuko HATA, Yoshiaki ONODERA, Noriaki OHUCHI, Hironobu SASANO
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌市
• [Presentation] Methylation and expression of the prostanoid receptor EP2 in ER+ breast cancer
  • Author(s): Sarah Q TO, Kiyoshi TAKAGI, Yasuhiro MIKI, Koyu SUZUKI, Eriko ABE, Yang YANG, Hironobu SASANO, Evan R SIMPSON, Colin D CLYNE, Kevin C KNOWER
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌市
• [Presentation] 乳癌におけるアロマターゼ阻害剤投与によるmicroRNA の変動およびLet-7f によるCYP19A1の制御に関する検討
  • Author(s): 柴原裕紀子、三木康宏、端 秀子、小野寺好明、中村保宏、赤平純一、阿部佳子、笹野公伸
  • Organizer: 第101回日本病理学会総会
  • Place of Presentation: 東京都