Investigation on the chemokine kinetics through red blood cells

• Project/Area Number: 23659292
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Laboratory medicine
• Research Institution: Hirosaki University
• Principal Investigator: KAYABA Hiroyuki 弘前大学, 医学(系)研究科(研究院), 教授 (70224706)
• Co-Investigator(Kenkyū-buntansha): CHIHARA Junichi 秋田大学, 大学院医学系研究科, 教授 (80197615)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 赤血球 / ケモカイン / アレルギー / 臨床マーカー / Duffy抗原 / 炎症

Research Abstract

Red Blood Cells (RBCs) express Duffy antigen receptor for chemokines (DARC) on the surface. DARC is a decoy receptor with no signal transduction into RBC and binds to several chemokines such as IL-8, MCP-1, Eotaxin and RANTES. RANTES was recognized in RBCs, but not on the surface. When compared the concentrations of IL-1beta;, TNF-alpha;, VEGF, RANTES, Eotaxin, MCP-1, IL-8 in the washed RBC supernatant before and after hemolysis, RANTES, Eotaxin and MCP-1 showed marked increase after hemolysis. This finding showed that RBCs absorb chemokines which have high affinity to DARC. RANTES was absorbed by RBCs rapidly. Intracellular RATNTES concentration reached almost the maximum level within 10 minutes. DARC expression decreased in the presence of RANTES. The expression of DARC and CD47 decreased in senescent RBCs.

Research Products

• [Journal Article] Flow cytometric analysis of red blood cell osmotic fragility (2014)
  • Author(s): Yamamoto A, SaitoN, YamauchiY, Masahide Takeda M, Ueki S, Itoga M, Kojima K, Kayaba H
  • Journal Title: J Lab Autom
• [Journal Article] 赤血球ケモカインの役割を探る (2014)
  • Author(s): 山本絢子、齋藤紀先、櫛引美穂子、小笠原脩、木津綾乃、蔦谷昭司、秋元広之、小島佳也、萱場広之
  • Journal Title: 日臨化東北会誌 Volume: 23 Pages: 18-21
• [Journal Article] Flow cytometric analysis of red blood cell osmotic fragility (2014)
  • Author(s): 1. Yamamoto A, Saito N, Yamauchi Y, Masahide Takeda M, Ueki S, Itoga M, Kojima K, Kayaba H
  • Journal Title: J Lab Autom Volume: 19
  • Peer Reviewed
• [Presentation] I型アレルギーと炎症マーカー (2013)
  • Author(s): 萱場広之、齋藤紀先、山本絢子、小島佳也
  • Organizer: 第53回日本臨床化学会年次学術集会
  • Place of Presentation: 徳島市
  • Year and Date: 2013-08-30
• [Presentation] Red Blood Cells absorb and store DARC-affinity chemokines (2012)
  • Author(s): KayabaH, ShiratoriT,YamautiY,Saito N, Chihara J, Kushibiki M, Akimoto H, Tsutaya S, Kojima K
  • Organizer: The 12th ASCPaLM
  • Place of Presentation: Kyoto,Japan
• [Presentation] アレルギー・炎症に赤血球が果たす役割は何か? (2011)
  • Author(s): 山内由美子、萱場広之、鎌田由美子、糸賀正道、竹田正秀、守時由紀、伊藤亘、茆原順一
  • Organizer: 第58回日本臨床検査医学会
  • Place of Presentation: 岡山
  • Year and Date: 2011-11-19
• [Presentation] フローサイトメトリーを用いた赤血球形態と膜抵抗評価(第二報) (2011)
  • Author(s): 萱場広之、山内由美子、鎌田由美子、糸賀正道、竹田正秀、守時由紀、伊藤亘、茆原順一
  • Organizer: 第58回日本臨床検査医学会
  • Place of Presentation: 岡山
  • Year and Date: 2011-11-18
• [Presentation] 赤血球がアレルギーに果たす役割に関する検討 (2011)
  • Author(s): 山内由美子、萱場広之、木原純子、鎌田由美子、茆原真美、糸賀正道、竹田正秀、小林良樹、守時由紀、伊藤亘、茆原順一
  • Organizer: 第61回日本アレルギー学会秋季学術大会
  • Place of Presentation: 東京
  • Year and Date: 2011-11-12
• [Presentation] The Features of Airway Remodeling Are More Severe in FemaleMice (2011)
  • Author(s): Takeda M, Ito W, Ueki S, Hirasawa H, Fujita M; Konno Y, Chihara M, Itoga M, Moritoki Y, Kobayashi Y, Kayaba H, Chihara J
  • Organizer: XXII World Allergy Congress
  • Place of Presentation: Cancun, Mexico
• [Presentation] I型アレルギーと炎症マーカー
  • Author(s): 5. 萱場広之、齋藤紀先、山本絢子、小島佳也
  • Organizer: 第53回日本臨床化学会年次学術集会
  • Place of Presentation: 徳島市
• [Presentation] 赤血球ケモカインの役割を探る
  • Author(s): 山本絢子、齋藤紀先、櫛引美穂子、小笠原脩、木津綾乃、蔦谷昭司、秋元広之、小島佳也、萱場広之
  • Organizer: 題24回日本臨床化学会東北支部総会
  • Place of Presentation: 仙台市
• [Presentation] Red Blood Cells Absorb and Store DARC-Binding Chemokines
  • Author(s): Hiroyuki Kayaba, Toshihiro Shiratori, Yumiko Yamauti, Norihiro Saito, Junichi Chihara , Mihoko Kushibiki, Hiroyuki Akimoto1 Shoji Tsutaya, Keiya Kojima
  • Organizer: The 12th Asian Society of Clinical Pathology and Laboratory Medicine
  • Place of Presentation: 京都
• [Presentation] アレルギー・炎症に赤血球が果たす役割は何か？
  • Author(s): 山内由美子、萱場広之、鎌田由美子、糸賀正道、竹田正秀、守時由紀、伊藤亘、茆原順一
  • Organizer: 第58回日本臨床検査医学会
  • Place of Presentation: 岡山
• [Presentation] フローサイトメトリーを用いた赤血球形態と膜抵抗評価（第二報）
  • Author(s): 萱場広之、山内由美子、鎌田由美子、糸賀正道、竹田正秀、守時由紀、伊藤亘、茆原順一
  • Organizer: 第58回日本臨床検査医学会
  • Place of Presentation: 岡山