Understanding of neuronal mechanisms of chronic pain

• Project/Area Number: 23659318
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Pain science
• Research Institution: Osaka University
• Principal Investigator: TOYODA Hiroki 大阪大学, 歯学研究科(研究院), 准教授 (00432451)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 慢性疼痛 / 帯状回 / シナプス / 興奮伝播 / EPSC / グルタミン酸受容体 / 帯状回皮質 / シナプス伝達

Research Abstract

The anterior cingulate cortex (ACC) is a forebrain structure that plays important roles in emotion, learning, memory and persistent pain. However, little is known about the neuronal mechanisms about chronic pain. In the present study, the quantal analysis of excitatory synapses in the ACC revealed that both probability of transmitter release and number of available vesicles were increased in a mouse model of peripheral inflammation. The voltage-sensitive dye imaging also revealed that the horizontal excitation spread in L2/3 caused by L4 stimulation in the ACC was enhanced in the mouse model of peripheral inflammation.

Research Products

• [Journal Article] A mathematical model of negative covariability of inter-columnar excitatory synaptic actions caused by presynaptic inhibition (2013)
  • Author(s): Saito M, Tanaka T, Sato H, Toyoda H, Aoyagi T, Kang Y
  • Journal Title: The European Journal of euroscience Volume: 38 Issue: 7 Pages: 2999-3007
  • DOI: 10.1111/ejn.12299
  • Peer Reviewed
• [Journal Article] GABAB receptor-mediated presynaptic inhibition reverses inter-columnar covariability of synaptic actions by intracortical axons in the rat barrel cortex (2013)
  • Author(s): Sato H, Toyoda H, Saito M, Kobayashi M, Althof D, Kulik A, Kang Y
  • Journal Title: The European Journal of Neuroscience Volume: 37 Issue: 2 Pages: 190-202
  • DOI: 10.1111/ejn.12041
  • Peer Reviewed
• [Journal Article] Illusion caused by vibration of muscle spindles reveals an involvement of muscle spindle inputs in regulating isometric contraction of masseter muscles (2012)
  • Author(s): Tsukiboshi T, Sato H, Tanaka Y, Saito M, Toyoda H, Morimoto T, Tiirker KS, Maeda Y, Kang Y
  • Journal Title: Journal of Neurophysiology Volume: 108 Issue: 9 Pages: 2524-2533
  • DOI: 10.1152/jn.00997.2011
  • Peer Reviewed
• [Journal Article] Capsaicin induces theta-band synchronization between gustatory and autonomic insular cortices (2012)
  • Author(s): Saito M, Toyoda H, Kawakami S, Sato H, Bae YC, Kang Y.
  • Journal Title: The Journal of Neuroscience Volume: 32 Issue: 39 Pages: 13470-13487
  • DOI: 10.1523/jneurosci.5906-11.2012
  • Peer Reviewed
• [Journal Article] Up-regulation of CREB-mediated transcription enhances both short- and long-term memory (2011)
  • Author(s): Suzuki, A., Fukushima, H., Takuya Mukawa, T., Toyoda, H., Wu, L-J., Zhao, M-G., Hui Xu, H., Shane, Y., Endoh, K., Iwamoto, Mamiya, N., Okano, E., Hasegawa, H., Mercaldo, V., Yue Zhang, Y., Maeda, R., Ohta, M., Josselyn, S.A., Zhuo, M., Kida, S.
  • Journal Title: J.Neurosci. Volume: 31 Issue: 24 Pages: 8786-8802
  • DOI: 10.1523/jneurosci.3257-10.2011
  • Peer Reviewed
• [Presentation] Phospholipase C-related inactive proteins are involved in the regulation of phasic and tonic inhibition in the barrel cortex (2013)
  • Author(s): Toyoda, H., Saito, M., Sato, H., Kanematsu, T., Hirata, M. and Kang, Y
  • Organizer: The 43rd Society for Neuroscience
  • Place of Presentation: San Diego, USA
  • Year and Date: 2013-11-11
• [Presentation] PRIP1/2 蛋白の発現抑制はバレル皮質錐体細胞において一過性GABA 抑制を強化する (2013)
  • Author(s): 豊田博紀, 齋藤充, 佐藤元, 兼松隆, 平田雅人, 姜英男
  • Organizer: 第55回歯科基礎医学会学術大会
  • Place of Presentation: 岡山
  • Year and Date: 2013-09-21
• [Presentation] Phospholipase C-related inactive protein regulates the Phasic and tonic inhibition in the barrel cortex (2013)
  • Author(s): Toyoda, H., Saito, M., Sato, H., Kanematsu, T., Hirata, M. and Kang, Y
  • Organizer: The 11lth Japan-Korea Joint Symposium of Brain Sciences, and Cardiac and Smooth Muscles
  • Place of Presentation: Hamamatsu
  • Year and Date: 2013-09-06
• [Presentation] バレル皮質錐体細胞においてPRIP 遺伝子欠損により細胞外シナプスGABAA 受容体の発現が増加する (2013)
  • Author(s): 豊田博紀, 齋藤充, 佐藤元, 兼松隆, 平田雅人, 姜英男
  • Organizer: 第36回日本神経科学大会
  • Place of Presentation: 京都
  • Year and Date: 2013-06-21
• [Presentation] バレル皮質錐体細胞においてPRIP 遺伝子欠損によりGABAA 電流の鋭感作および再感作が増強する (2013)
  • Author(s): 豊田博紀, 小川丈夫, 佐藤元, 齋藤充, 兼松隆, 平田雅人, 姜英男
  • Organizer: 第90回日本生理学会大会
  • Place of Presentation: 東京
  • Year and Date: 2013-03-27
• [Presentation] バレル皮質錐体細胞においてPRIP遺伝子欠損により細胞外シナプスGABAA受容体の発現が増加する (2013)
  • Author(s): 豊田博紀 他
  • Organizer: 第36回日本神経科学大会
  • Place of Presentation: 京都国際会館（京都）
• [Presentation] Phospholipase C-related inactive protein regulates the phasic and tonic inhibition in the barrel cortex (2013)
  • Author(s): Toyoda, H. et al
  • Organizer: The 11th Japan-Korea Joint Symposium of Brain Sciences, and Cardiac and Smooth Muscles
  • Place of Presentation: 浜松アクトシティ（浜松）
• [Presentation] PRIP1/2蛋白の発現抑制はバレル皮質錐体細胞において一過性GABA抑制を強化する (2013)
  • Author(s): 豊田博紀 他
  • Organizer: 第55回歯科基礎医学会
  • Place of Presentation: 岡山コンベンションセンター（岡山）
• [Presentation] Phospholipase C-related inactive proteins are involved in the regulation of phasic and tonic inhibition in the barrel cortex (2013)
  • Author(s): Toyoda, H. et al
  • Organizer: 43rd Society for Neuroscience
  • Place of Presentation: San Diego convention center（San Diego）
• [Presentation] バレル皮質錐体細胞においてPRIP遺伝子欠損によりGABA(A)電流の脱感作および再感作が増強する (2013)
  • Author(s): 豊田博紀 他
  • Organizer: 第90回日本生理学会大会
  • Place of Presentation: タワーホール船堀（東京）
• [Presentation] Phospholipase C-related inactive protein resensitization of GABA_A receptor currents in the barrel cortex (2012)
  • Author(s): Toyoda, H., Ogawa, T., Saito, M., Sato, H., Kanematsu, T., Hirata, M. and Kang, Y
  • Organizer: The 42nd Society for Neuroscience
  • Place of Presentation: New Orleans, USA
  • Year and Date: 2012-10-14
• [Presentation] PRIP 遺伝子欠損によりバレル皮質錐体細胞におけるGABAA 電流の脱感作および再感作が増強する (2012)
  • Author(s): 豊田博紀, 小川丈夫, 齋藤充, 佐藤元, 兼松隆, 平田雅人, 姜英男
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 2012-09-18
• [Presentation] PRIP遺伝子欠損によりバレル皮質錐体細胞におけるGABA(A)電流の脱感作および再感作が増強する (2012)
  • Author(s): 豊田博紀 他
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋国際会議場（名古屋）
• [Presentation] Phospholipase C-related inactive protein modulates desensitization and resensitization of GABAA receptor currents in the barrel cortex (2012)
  • Author(s): Toyoda, H. et al.
  • Organizer: 42th Society for Neuroscience
  • Place of Presentation: Ernest N. Morial Convention Center (New Orleans, USA)
• [Presentation] cGMP differentially modulates leak K^+ currents in masseter motoneurons (2011)
  • Author(s): Toyoda, H., Hirao, K., Saito M., Sato, H and Kang, Y
  • Organizer: The 41th Society for Neuroscience
  • Place of Presentation: Washington, USA
  • Year and Date: 2011-11-13
• [Presentation] バレル皮質におけるGABAA 受容体電流および興奮伝播に対するPRIP の役割 (2011)
  • Author(s): 豊田博紀, 小川丈夫, 齋藤充, 佐藤元, 兼松隆, 平田雅人, 姜英男
  • Organizer: 第53回歯科基礎医学会学術大会
  • Place of Presentation: 岐阜
  • Year and Date: 2011-10-02
• [Presentation] Oscillatory neural synchronization between the gustatory and visceral insular cortices induced by activation of TRPVl in the gustatory area (2011)
  • Author(s): Toyoda, H. and Kang, Y
  • Organizer: The 6th international conference of neurons and brain diseases
  • Place of Presentation: Toyama
  • Year and Date: 2011-08-03
• [Presentation] Oscillatory neural synchronization between the gustatory and visceral insular cortices induced by activation of TRPV1 in the gustatory area (2011)
  • Author(s): Toyoda, H. et al.
  • Organizer: The 6th international conference of Neurons and Brain diseases(招待講演)
  • Place of Presentation: ANAクラウンプラザ富山（富山県）
• [Presentation] cGMP differentially modulates leak K+ currents in masseter motoneurons (2011)
  • Author(s): Toyoda, H. et al.
  • Organizer: 41th Society for Neuroscience
  • Place of Presentation: Washington convention center (Washington, USA)
• [Presentation] バレル皮質におけるGABA(A)受容体電流および興奮伝播に対するPRIPの役割 (2011)
  • Author(s): Toyoda, H. et al.
  • Organizer: 第53回歯科基礎医学会
  • Place of Presentation: 長良川国際会議場（岐阜）