New classification of nociceptive sensory neurons and functional interaction between TRPV1 and TRPA1

• Project/Area Number: 23659323
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Pain science
• Research Institution: 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設) (2012); National Institutes of Natural Sciences Okazaki Research Facilities (2011)
• Principal Investigator: TOMINAGA Makoto 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 教授 (90260041)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 侵害受容器 / 疼痛学

Research Abstract

We found that capsiate, an ingredient contained in non-pungent capsicum, activates TRPA1. We found that Primary alcohols activate human TRPA1 channel in a carbon chain length-dependent manner. We found that two isothiocyanates from Wasabia japonica (6-MSITC, 6-MTITC) activate TRPA1. We found that 1,8-cineole, a TRPM8 agonist, is a novel natural antagonist of human TRPA1 upon intensive screening.

Research Products

• [Journal Article] Activation of transient receptor potential A1 by a non-pungent capsaicin-like compound, capsiate. (2012)
  • Author(s): Shintaku K and Uchida K et al.
  • Journal Title: British Journal of Pharmacology Volume: 165(5) Issue: 5 Pages: 1476-1486
  • DOI: 10.1111/j.1476-5381.2011.01634.x
  • Peer Reviewed
• [Journal Article] Primary alcohols activate human TRPA1 channel in a carbon chain length-dependent manner (2012)
  • Author(s): Komatsu T, Uchida K, Fujita F, Zhou Y, Tominaga M
  • Journal Title: European Journal of Physiology Volume: 463 Issue: 4 Pages: 549-559
  • DOI: 10.1007/s00424-011-1069-4
  • Peer Reviewed
• [Journal Article] Isothiocyanates from Wasabia japonica activate transient receptor potential ankyrin 1 channel. (2012)
  • Author(s): Uchida K, Miura Y, Nagai M & Tominaga M.
  • Journal Title: Chemical Senses Volume: 37 Issue: 9 Pages: 809-818
  • DOI: 10.1093/chemse/bjs065
  • Peer Reviewed
• [Journal Article] 1,8-cineole, a TRPM8 agonist, is a novel natural antagonist of human TRPA1 (2012)
  • Author(s): Takaishi M, Fujita F, Uchida K, Yamamoto S, Sawada M, Hatai C, Shimizu M & Tominaga M.
  • Journal Title: Molecular Pain Volume: 8 Pages: 86-86
  • Peer Reviewed
• [Journal Article] 1,8-cineole, a TRPM8 agonist, is a novel natural antagonist of human TRPA1. (2012)
  • Author(s): Takaishi M, Fujita F, Uchida K, Yamamoto S, Sawada M, Hatai C, Shimizu M & Tominaga M
  • Journal Title: Molecular Pain Volume: 8 Pages: 86-86
  • Peer Reviewed
• [Presentation] ワサビから抽出される種々のイソチオシアネートによるTRPA1の活性化 (2012)
  • Author(s): 内田邦敏、三浦陽介、永井雅、富永真琴、
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 愛知
  • Year and Date: 2012-09-20
• [Presentation] 辛くないトウガラシ成分カプシエイトによるTRPA1の活性化 (2011)
  • Author(s): 新宅健司、富永真琴
  • Organizer: 第33回日本疼痛学会
  • Place of Presentation: 愛知
  • Year and Date: 2011-07-23
• [Presentation] 辛くないトウガラシ成分カプシエイトによるTRPA1の活性化 (2011)
  • Author(s): 新宅健司、富永真琴
  • Organizer: 第33回日本疼痛学会(招待講演)
  • Place of Presentation: 愛媛県民文化会館(愛媛県)
• [Presentation] TRPチャネルの侵害刺激受容と鎮痛薬開発標的の可能性 (2011)
  • Author(s): 富永真琴
  • Organizer: 日本ペインクリニック学会第45回大会
  • Place of Presentation: 愛媛県民文化会館(愛媛県)
• [Presentation] ワサビから抽出される種々のイソチオシアネートによるTRPA1の活性化.
  • Author(s): 内田邦敏、三浦陽介、永井雅、富永真琴
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋国際会議場（愛知県）
• [Remarks]
  • URL: http://www.nips.ac.jp/cs
• [Remarks] 自然科学研究機構 岡崎統合バイオサイエンスセンター 細胞生理研究部門
  • URL: http://www.nips.ac.jp/cs/