Development of new protective method against environmental factors.

• Project/Area Number: 23659324
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Hygiene
• Research Institution: Hokkaido University
• Principal Investigator: FUJITA Hiroyoshi 北海道大学, 医学(系)研究科(研究院), 教授 (60142931)
• Co-Investigator(Kenkyū-buntansha): WAKAO Hiroshi 北海道大学, 大学院医学研究科, 准教授 (10280950) ; ODA Atsushi 北海道大学, 大学院医学研究科, 准教授 (50255436)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: MAIT細胞 / iPS化 / 多剤耐性菌対策 / 細胞治療 / 抗菌活性 / 抗酸菌 / ゲノム修飾のないiPS化 / 粘膜関連インバリアントT細胞 / 分化誘導 / NKT細胞 / さる胚性幹細胞 / レンチウイルスベクター / T細胞分化誘導

Research Abstract

We investigated anti-mycobacterial activity of human reMAIT cells. Immunocompromised mice received human reMAIT cells could resist against M. abscessus infection. reMAIT cells transfered mice demonstrated marked expression of granulysin whose gene was absent in mice, indicating that human reMAIT cells killed mycobacteria through granylysin. Hereby,a novel method against infectious diseases is demonstrated.

Research Products

• [Journal Article] Expansion of functional human Mucosal-Associated Invariant T Cells via reprogramming to pluripotency and redifferentiation (2013)
  • Author(s): H Wakao, K Yoshikiyo, U Koshimizu, T Furukawa, K Enomoto, T Matsunaga, T Tanaka, Y Yasutomi, T Yamada, H Minakami, J Tanaka, A Oda, T Sasaki, R Wakao, O Lantz, T Udagawa, Y Sekiya, K Higuchi, N Harada,K Nishimura, M Ohtaka, M Nakanishi and H Fujita
  • Journal Title: Cell Stem Cell Volume: 12 Pages: 546-558
  • Peer Reviewed
• [Journal Article] Toward the realization of cell therapy; the advent of MAIT cells from iPSCs (2013)
  • Author(s): H Wakao and H Fujita
  • Journal Title: Cell Cycle Volume: 12 Pages: 2341-2342
  • Peer Reviewed
• [Journal Article] Toward the realization of cell therapy; the advent of MAIT cells from iPSCs. (2013)
  • Author(s): H Wakao and H Fujita
  • Journal Title: Cell Cycle Volume: 12 Pages: 2341-2342
  • Peer Reviewed
• [Journal Article] Expansion of Functional Human Mucosal-Associated Invariant T Cells via Reprogramming to Pluripotency and Redifferentiation (2013)
  • Author(s): H Wakao, K Yoshikiyo, U Koshimizu et al. and H Fujita
  • Journal Title: Cell Stem Cell Volume: in press
  • Peer Reviewed
• [Journal Article] 衛生学の見た夢 (2012)
  • Author(s): 藤田博美
  • Journal Title: 北海道医学雑誌 Volume: 87 Pages: 173-174
  • NAID: 10030993082
  • Peer Reviewed
• [Presentation] The advent of mucosal-associated invariant T (WAIT) cells from iPSCs opens a novel avenue for immunology (2013)
  • Author(s): H Wakao, K Yoshikiyo, U Koshimizu, K Enomoto, T Tanaka, Y Yasutomi, H Fujita
  • Organizer: 15^<th> International Congress of Immunology
  • Place of Presentation: Milan, Italy
• [Presentation] ガン化のおそれのないiPS 細胞の開発 (2013)
  • Author(s): 若尾宏、藤田博美
  • Organizer: 第83回日本衛生学会学術総会
  • Place of Presentation: 金沢
• [Presentation] ガン化のおそれのないヒトiPS細胞作製の試み (2013)
  • Author(s): 若尾宏、藤田博美
  • Organizer: 日本衛生学会学術総会
  • Place of Presentation: 金沢大学・金沢美術工芸大学（金沢市）
• [Presentation] Constitutively active Stat5a and Stta5b promote adipogenesis (2011)
  • Author(s): 若尾 宏
  • Organizer: cytokine receptors and their Stat-mediated mechanisms of action(招待講演)
  • Place of Presentation: GEORG SPEYER HAUS(Germany)
• [Remarks]
  • URL: http://www.hokudai.ac.jp/news/130322_pr_med.pdf
• [Remarks] PRESS RELEASE (2013/3/22)
  • URL: http://www.hokudai.ac.jp/news/130322_pr_med.pdf
• [Patent(Industrial Property Rights)] MAIT-like cells and method for manufacturing same. (2013)
  • Inventor(s): 若尾宏，藤田博美、小清水右一，由清和則
  • Industrial Property Rights Holder: 若尾宏，藤田博美、小清水右一，由清和則
  • Industrial Property Rights Type: 特許
  • Filing Date: 2013-10-30
  • Acquisition Date: 2014-05-08
  • Overseas
• [Patent(Industrial Property Rights)] MAIT 様細胞およびその作成方法 (2012)
  • Inventor(s): 若尾宏,小清水右一、吉清和則、藤田博美
  • Industrial Property Rights Holder: 若尾宏,小清水右一、吉清和則、藤田博美
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2012-239195
  • Filing Date: 2012-10-30