Development of combination methods with dendritic cells and novel chemokine products for preventing hepatocellular carcinoma recurrence

• Project/Area Number: 23659396
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Gastroenterology
• Research Institution: University of Fukui
• Principal Investigator: NAKAMOTO Yasunari 福井大学, 医学部, 教授 (40293352)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 癌 / 免疫学 / ﾄﾗﾝｽﾚｰｼｮﾅﾙﾘｻｰﾁ / 動物 / 内科 / トランスレーショナルリサーチ

Research Abstract

The current study was designed to develop the combination methods with dendritic cells and novel chemokine products for preventing the recurrence of hepatocellular carcinoma following the treatments. The methods of dendritic cell induction were modified using OK-432 and zinc, and the chemokine products were evaluated for the activation of the cancer immunity. Furthermore, the biological stability of the products was improved by recombinant technology. Collectively, the results indicated that the modified methods using both dendritic cells and the chemokine products may contribute to the reduction of hepatocellular carcinoma recurrence.

Research Products

• [Journal Article] Gd-EOB-DTPA-enhanced magnetic resonance imaging and alpha-fetoprotein predict prognosis of early-stage hepatocellular carcinoma. (2014)
  • Author(s): Yamashita T, Kitao A, Matsui O, Hayashi T, Nio K, Kondo M, Ohno N, Miyati T, Okada H, Yamashita T, Mizukoshi E, Honda M, Nakanuma Y, Takamura H, Ohta T, Nakamoto Y, Yamamoto M, Takayama T, Arii S, Wang XW, Kaneko S
  • Journal Title: Hepatology Volume: in press Issue: 5 Pages: 1674-85
  • DOI: 10.1002/hep.27093
  • Peer Reviewed / Open Access
• [Journal Article] Multicenter validation study of anti-programmed cell death-1 antibody as a serological marker for type 1 autoimmune hepatitis. (2014)
  • Author(s): Miyake Y, Yamamoto K, Matsushita H, Abe M, Takahashi A, Umemura T, Tanaka A, Nakamuta M, Nakamoto Y, Ueno Y, Saibara T, Takikawa H, Yoshizawa K, Ohira H, Zeniya M, Onji M, Tsubouchi H; Intractable Liver and Biliary Diseases Study Group of Japan
  • Journal Title: Hepatol Res. Volume: in press Issue: 13 Pages: 1299-1307
  • DOI: 10.1111/hepr.12305
  • Peer Reviewed / Open Access
• [Journal Article] In vivo immunological antitumor effect of OK-432-stimulated dendritic cell transfer after radiofrequency ablation. (2014)
  • Author(s): Nakagawa H, Mizukoshi E, Iida N, Terashima T, Kitahara M, Marukawa Y, KitamuraK, Nakamoto Y, Hiroishi K, Imawari M, Kaneko S
  • Journal Title: Cancer Immunol Immunother. Volume: 63(4) Issue: 4 Pages: 347-356
  • DOI: 10.1007/s00262-013-1514-7
  • NAID: 120005368239
  • Peer Reviewed / Open Access
• [Journal Article] Pathophysiological Decrease in the Regional Cerebral Blood Flow in Hashimoto's Encephalopathy : A Multiple-Case SPECT Study (2014)
  • Author(s): Muramatsu T, Ikawa M, Yoneda M, Sugimoto K, Matsunaga A, Yamamura O, Hamano T, Okazawa H, Nakamoto Y
  • Journal Title: Eur Neurol Volume: 72 Pages: 13-19
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/24777056
  • Peer Reviewed
• [Journal Article] Discrete nature of EpCAM(+) and CD90(+) cancer stem cells in human hepatocellular carcinoma. (2013)
  • Author(s): Yamashita T, Nakamoto Y, et al.
  • Journal Title: Hepatology Volume: 57 Issue: 4 Pages: 1484-1497
  • DOI: 10.1002/hep.26168
  • Peer Reviewed
• [Journal Article] Increase in CD14+HLA-DR -/low myeloid-derived suppressor cells in hepatocellular carcinoma patients and its impact on prognosis. (2013)
  • Author(s): Arihara F, Mizukoshi E, Kitahara M, Takata Y, Arai K, Yamashita T, Nakamoto Y, Kaneko S
  • Journal Title: Cancer Immunol Immunother. Volume: 62(8) Issue: 8 Pages: 1421-1430
  • DOI: 10.1007/s00262-013-1447-1
  • Peer Reviewed
• [Journal Article] Correction : TNF-α and Tumor Lysate Promote the Maturation of Dendritic Cells for Immunotherapy for Advanced Malignant Bone and Soft Tissue Tumors (2013)
  • Author(s): Miwa S, Nishida H, Tanzawa Y, Takata M, Takeuchi A, Yamamoto N, Shirai T, Hayashi K, Kimura H, Igarashi K, Mizukoshi E, Nakamoto Y, Kaneko S, Tsuchiya H
  • Journal Title: PLoS One (eCollection 2013.) Volume: 24 Pages: 8-8
  • Peer Reviewed
• [Journal Article] Detection of preclinically latent hyperperfusion due to stroke-like episodes by arterial spin-labeling perfusion MRI in MELAS patients. (2013)
  • Author(s): Masamichi Ikawa, Makoto Yoneda, Tomoko Muramatsu, Akiko Matsunaga, Tetsuya Tsujikawa, Tatsuya Yamamoto, Nobuyuki Kosaka, Kazuyuki Kinoshita, Osamu Yamamura, Tadanori Hamano, Yasunari Nakamoto, Hirohiko Kimura
  • Journal Title: Mitochondrion Volume: 13(6) Issue: 6 Pages: 676-680
  • DOI: 10.1016/j.mito.2013.09.007
  • Peer Reviewed
• [Journal Article] Hashimoto's encephalopathy as a treatable adult-onset cerebellar ataxia mimicking spinocerebellar degeneration (2013)
  • Author(s): Matsunaga A, Ikawa M, Fujii A, Nakamoto Y, Kuriyama M, Yoneda M
  • Journal Title: Eur. Neurol Volume: 69 Issue: 1 Pages: 14-20
  • DOI: 10.1159/000342217
  • Peer Reviewed
• [Journal Article] TNF-α and tumor lysate promote the maturation of dendritic cells for immunotherapy for advanced malignant bone and soft tissue tumors (2012)
  • Author(s): Miwa S, Tsuchiya H, et al
  • Journal Title: PLoS ONE Volume: 7 Issue: 12 Pages: e52926-e52926
  • DOI: 10.1371/journal.pone.0052926
  • Peer Reviewed
• [Journal Article] Clinical relevance and sequence analysis of the Helicobacter pylori dupA region from two areas in Japan with different gastric cancer risks (2012)
  • Author(s): Matsuda H, Ito Y, Suto H, Yamakawa A, Satomi S, Ohtani M Yamazaki Y, Kusaka Y, Shimabukuro Y, Kikuchi K, Keida Y, Azuma T, Nakamoto Y
  • Journal Title: Immuno-Gatroenterology Volume: 1:2 Pages: 127-135
  • URL: http://www.stmconnect.com/ig
  • Peer Reviewed
• [Journal Article] Membrane-bound form of monocyte chemoattractant protein-1 enhances antitumor effects of suicide gene therapy in a model of hepatocellular carcinoma. (2012)
  • Author(s): Marukawa Y
  • Journal Title: Cancer Gene Ther. Volume: 19(5) Issue: 5 Pages: 312-319
  • DOI: 10.1038/cgt.2012.3
  • Peer Reviewed
• [Journal Article] Nucleostemin in injury-induced liver regeneration. (2012)
  • Author(s): Shugo. H
  • Journal Title: Stem cells Dev. Volume: 21 Issue: 16 Pages: 3044-3054
  • DOI: 10.1089/scd.2011.0725
  • Peer Reviewed
• [Journal Article] Guideline on the use of new anticancer drugs for the treatment of Hepatocellular Carcinoma 2010 update (2012)
  • Author(s): Kaneko S, Furuse J, Kudo M, Ikeda K, Honda M, Nakamoto Y, Onchi M, Shiota G, Yokosuka O, Sakaida I, Takehara T, Ueno Y, Hiroishi K, Nishiguchi S, Moriwaki H, Yamamoto K, Sata M, Obi S, Miyayama S, Imai Y
  • Journal Title: Hepatol. Res Volume: 42 Issue: 6 Pages: 523-542
  • DOI: 10.1111/j.1872-034x.2012.00981.x
  • NAID: 10031144267
  • Peer Reviewed
• [Journal Article] Evaluation of systemic redox states in patients carrying MELAS A3243G mutation in mitochondrial DNA. (2012)
  • Author(s): Masamichi Ikawa, Kenichiro Arakawa, Tadanori Hamano, Miwako Nagata, Yasunari Nakamoto, Masaru Kuriyama, Yasutoshi Koga, Makoto Yoneda.
  • Journal Title: European Neurology Volume: 67(4) Issue: 4 Pages: 232-7
  • DOI: 10.1159/000336568
  • Peer Reviewed
• [Journal Article] Prolonged recurrence-free survival following OK432-stimulated dendritic cell transfer into hepatocellular carcinoma during transarterial embolization (2011)
  • Author(s): Nakamoto Y, Mizukoshi E, Kitahara M, Arihara F, Sakai Y, Kakinoki K, Fujita Y, Marukawa Y, Arai K, Yamashita T, Mukaida N, Matsushima K, Matsui O, and Kaneko S
  • Journal Title: Clin Exp Immunol Volume: 163(2) Issue: 2 Pages: 165-177
  • DOI: 10.1111/j.1365-2249.2010.04246.x
  • NAID: 120003141443
  • Peer Reviewed
• [Journal Article] Comparative analysis of various tumor-associated antigen-specific t-cell responses in patients with hepatocellular carcinoma (2011)
  • Author(s): Honda M, (他9名, 9番目)
  • Journal Title: Hepatology Volume: 53(4) Issue: 4 Pages: 206-16
  • DOI: 10.1002/hep.24149
  • Peer Reviewed
• [Journal Article] Frequency of CD45RO+ subset in CD4+CD25(high) regulatory T cells associated with progression of hepatocellular carcinoma (2011)
  • Author(s): Takata Y, Nakamoto Y, Nakada A, Terashima T, Arihara F, Kitahara M, Kakinoki K, Arai K, Yamashita T, Sakai Y, Yamashita T, Mizukoshi E, Kaneko S
  • Journal Title: Cancer Lett Volume: 307 Issue: 2 Pages: 165-173
  • DOI: 10.1016/j.canlet.2011.03.029
  • NAID: 120003141809
  • Peer Reviewed
• [Journal Article] Malnutrition Impairs Interferon Signaling Through mTOR and FoxO Pathways in Patients With Chronic Hepatitis C (2011)
  • Author(s): Honda M, 他19名
  • Journal Title: Gastroenterology Volume: 141(1) Issue: 1 Pages: 128-40
  • DOI: 10.1053/j.gastro.2011.03.051
  • NAID: 40019193331
  • Peer Reviewed
• [Journal Article] Randomized, phase II study comparing interferon combined with hepatic arterial infusion of fluorouracil plus cisplatin and fluorouracil alone in patients with advanced hepatocellularcar cinoma (2011)
  • Author(s): Honda M, (他10名, 10番目)
  • Journal Title: Oncology Volume: 81(5-6) Issue: 5-6 Pages: 281-90
  • DOI: 10.1159/000334439
  • NAID: 120003674297
  • Peer Reviewed
• [Journal Article] Frequency of CD45RO(+) subset in CD4(+)CD25(high) regulatory T cells associated with progression of hepatocellular carcinoma. (2011)
  • Author(s): Takata Y, Nakamoto Y, et al.
  • Journal Title: Cancer Lett. Volume: 307 Pages: 165-173
  • NAID: 120003141809
  • Peer Reviewed
• [Presentation] Clinical evaluation of patency capsule system in high risk patients for video capsule endoscopy (2013)
  • Author(s): Ohtani M, Suto H, Nakamoto Y
  • Organizer: Japan Digestive DiseaseWeek 2013
  • Place of Presentation: Tokyo
  • Year and Date: 2013-10-12
• [Presentation] MicroRNA Dynamics in Precancerous Lesions in a Mouse Model of Chronic Hepatitis B (2012)
  • Author(s): Nakamoto Y, Yamashita T, Kaneko S
  • Organizer: 第63回American Association for the Study of Liver Diseases (AASLD) Annual Meeting
  • Place of Presentation: Boston, Massachusetts, Hepatology 56 (4,Suppl.) 315A
  • Year and Date: 2012-11-13
• [Presentation] High Incidence of HBV Reactivation after R-CHOP and CHOP Regimens among Patients Treated with Six Immunosuppressive Chemotherapies (2012)
  • Author(s): Naito T, Nemoto T, Matsuda H, Ohtani M, Suto H Nakamoto Y
  • Organizer: 第63回American Association for the Study of Liver Diseases (AASLD) Annual Meeting
  • Place of Presentation: Boston, Massachusetts, Hepatology 56 (4,Suppl.) 643A
  • Year and Date: 2012-11-11
• [Presentation] Differential Dynamics of the NF-kappaB subunits RELA and RELB in a Mouse Model of Chronic Hepatitis B (2011)
  • Author(s): Nakamoto Y, Yamashita T, Kaneko S
  • Organizer: 第62回American Association for the Study of Liver Diseases (AASLD) Annual Meeting
  • Place of Presentation: San Francisco, California, Hepatology 54 (4,Suppl.) 1300A
  • Year and Date: 2011-11-08
• [Presentation] Increase of CD14+HLA-DR-/low myeloid-derived suppressor cells in hepatocellular carcinoma and its impact for prognosis of patients (2011)
  • Author(s): Arihara F, Mizukoshi E, Kitahara M, Takata Y, Arai K, Yamashita T, Nakamoto Y, Kaneko S
  • Organizer: 第62回American Association for the Study of Liver Diseases (AASLD) Annual Meeting
  • Place of Presentation: San Francisco, California, Hepatology 54 (4,Suppl.) 1400A
  • Year and Date: 2011-11-08
• [Presentation] Differential Dynamics of the NF-kappaB subunits RELA and RELB in a Mouse Model of Chronic Hepatitis B. (2011)
  • Author(s): Nakamoto Y, Yamashita T, Kaneko S
  • Organizer: 第62回American Association for the Study of Liver Diseases (AASLD) Annual Meeting
  • Place of Presentation: San Francisco, California
• [Presentation] Clinical evaluation of patency capsule system in high risk patients for video capsule endoscopy.
  • Author(s): Ohtani M, Suto H, Nakamoto Y
  • Organizer: Japan Digestive Disease Week 2013
  • Place of Presentation: Grand Prince Hotel New Takanawa, Tokyo
• [Presentation] MicroRNA Dynamics in Precancerous Lesions in a Mouse Model of Chronic Hepatitis B.
  • Author(s): Nakamoto Y, Yamashita T, Kaneko S
  • Organizer: 第63回 American Association for the Study of Liver Diseases (AASLD) Annual Meeting
  • Place of Presentation: Boston, Massachusetts, U.S.A.