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Anti KL-6 antibody enhances the effects of molecular targeted drug

Research Project

Project/Area Number 23659433
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Respiratory organ internal medicine
Research InstitutionHiroshima University

Principal Investigator

KOHNO Nobuoki  広島大学, 医歯薬保健学研究院(医), 教授 (80215194)

Co-Investigator(Renkei-kenkyūsha) HATTORI Noboru  広島大学, 医歯薬保健学研究院(医), 准教授 (00283169)
ISHIKAWA Nobuhisa  広島大学, 医歯薬保健学研究院(医), 講師 (90423368)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
KeywordsKL-6 / 分子標的治療薬 / 肺癌
Research Abstract

The aim of the present study was to investigate the additive effect of anti-KL-6 antibody on the therapeutic antibody-induced ADCC (Antibody-Dependent Cellular-Cytotoxicity) activity. The expression of HER2 and KL-6 were measured using FACS analysis in 4 cancer cell lines (SK-BR-3, ZR75-1, PC3, YMB-S), and both HER2 and KL-6 were highly expressed in YMB-S cells. Accordingly, the effects of anti KL-6 antibody on the ADCC activity of PBMCs evoked by Trastuzumab were examined using Cr-51 release assay. Enhanced cytotoxicity was observed in an effector-to-target ratio manner when YMB-S cells were treated with anti-KL-6 antibody. These findings indicate that anti KL-6 antibody can enhance the therapeutic antibody-induced ADCC activity mediated by mononuclear cells.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (37 results)

All 2014 2013 2012 2011

All Journal Article (17 results) (of which Peer Reviewed: 17 results,  Open Access: 1 results) Presentation (19 results) (of which Invited: 1 results) Book (1 results)

  • [Journal Article] Differences in serum SP-D levels between German and Japanese subjects are associated with SFTPD gene polymorphisms.2014

    • Author(s)
      Horimasu Y, Hattori N, Ishikawa N, Tanaka S, Bonella F, Ohshimo S, Guzman J, Costabel U, Kohno N
    • Journal Title

      BMC Med Genet

      Volume: 15 Issue: 1 Pages: 4-4

    • DOI

      10.1186/1471-2350-15-4

    • NAID

      120006799353

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] SK-216, an inhibitor of plasminogen activator inhibitor-1, limits tumor progression and angiogenesis2013

    • Author(s)
      Masuda T, Hattori N, Senoo T, Akita S, Ishikawa N, Fujitaka K, Haruta Y, Murai H, Kohno N
    • Journal Title

      Mol Cancer Ther

      Volume: 12 Pages: 2378-88

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] SK-216, an inhibitor of plasminogen activator inhibitor-1, limits tumor progression and angiogenesis.2013

    • Author(s)
      Masuda T, Hattori N, Senoo T, Akita S, Ishikawa N, Fujitaka K, Haruta Y, Murai H, Kohno N.
    • Journal Title

      Mol Cancer Ther

      Volume: 12 Issue: 11 Pages: 2378-88

    • DOI

      10.1158/1535-7163.mct-13-0041

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characteristics of inspiratory and expiratory reactance in interstitial lung disease.2013

    • Author(s)
      Sugiyama A, Hattori N, Haruta Y, Nakamura I, Nakagawa M, Miyamoto S, Onari Y, Iwamoto H, Ishikawa N, Fujitaka K, Murai H, Kohno N.
    • Journal Title

      Respir Med

      Volume: 107 Issue: 6 Pages: 875-82

    • DOI

      10.1016/j.rmed.2013.03.005

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A nationwide epidemiological survey of chronic hypersensitivity pneumonitis in Japan.2013

    • Author(s)
      Okamoto T, Miyazaki Y, Ogura T, Chida K, Kohno N, Kohno S, Taniguchi H, Akagawa S, Mochizuki Y, Yamauchi K, Takahashi H, Johkoh T, Homma S, Kishi K, Ikushima S, Konno S, Mishima M, Ohta K, Nishioka Y, Yoshimura N, Munakata M, Watanabe K, Miyashita Y, Inase N.
    • Journal Title

      Respir Investig

      Volume: 51 Pages: 191-199

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] RASEF is a novel diagnostic biomarker and a therapeutic target for lung cancer.2013

    • Author(s)
      Oshita H, Nishino R, Takano A, Fujitomo T, Aragaki M, Kato T, Akiyama H, Tsuchiya E, Kohno N, Nakamura Y, Daigo Y.
    • Journal Title

      Mol Cancer Res

      Volume: 11 Issue: 8 Pages: 937-951

    • DOI

      10.1158/1541-7786.mcr-12-0685-t

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cross-sectional and prospective study of the association between lung function and prediabetes2013

    • Author(s)
      Yamane T, Yokoyama A, Kitahara Y*, Miyamoto S, Haruta Y, Hattori N, Yamane K, Hara H, Kohno N
    • Journal Title

      BMJ Open

      Volume: 3 Issue: 2 Pages: 3-3

    • DOI

      10.1136/bmjopen-2012-002179

    • NAID

      120006799354

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Krebs von den Lungen-6 (KL-6) is a prognostic biomarker in patients with surgically resected non-small cell lung cancer.2012

    • Author(s)
      Tanaka S, et al.
    • Journal Title

      International journal of cancer

      Volume: 130 Issue: 2 Pages: 377-387

    • DOI

      10.1002/ijc.26007

    • Related Report
      2013 Final Research Report 2012 Research-status Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Interferon (alpha, beta and omega) receptor 2 is a prognostic biomarker for lung cancer2012

    • Author(s)
      Tanaka S, Hattori N, Ishikawa N, Horimasu Y, Deguchi N, Takano A, Tomoda Y, Yoshioka K, Fujitaka K, Arihiro K, Okada M, Yokoyama A, Kohno N
    • Journal Title

      Pathobiology

      Volume: 79 Issue: 1 Pages: 24-33

    • DOI

      10.1159/000331230

    • Related Report
      2013 Final Research Report 2012 Research-status Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Different MUC1 gene polymorphisms in German and Japanese ethnicities affect serum KL-6 levels2012

    • Author(s)
      Horimasu Y, Hattori N, Ishikawa N, K awase S, Tanaka S, Yoshioka K, Yokoy ama A, Kohno N, Bonella F, Guzman J, Ohshimo S, Costabel U
    • Journal Title

      Respir Med

      Volume: 106 Issue: 12 Pages: 1756-64

    • DOI

      10.1016/j.rmed.2012.09.001

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Utility of KL-6/MUC1 in the clinical management of interstitial lung diseases2012

    • Author(s)
      Ishikawa N, Hattori N, Yokoyama A, Kohno N
    • Journal Title

      Respir Investig

      Volume: 50 Issue: 1 Pages: 3-13

    • DOI

      10.1016/j.resinv.2012.02.001

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Comparison of serum KL-6 versus bronchoalveolar lavage neutrophilia for the diagnosis of bronchiolitis obliterans in lung transplantation.2011

    • Author(s)
      Ohshimo S, Bonella F, Sommerwerck U, Teschler H, Kamler M, Jakob HG, Kohno N, Guzman J, Costabel U.
    • Journal Title

      J Heart Lung Transplant

      Volume: 30 Issue: 12 Pages: 1374-1380

    • DOI

      10.1016/j.healun.2011.07.010

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Change in serum KL-6 level from baseline is useful for predicting life-threatening EGFR-TKIs induced interstitial lung disease2011

    • Author(s)
      Kawase S, et al
    • Journal Title

      Respir Res

      Volume: 12 Issue: 1 Pages: 97-97

    • DOI

      10.1186/1465-9921-12-97

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Ageing and long-term smoking affects KL-6 levels in the lung, induced sputum and plasma.2011

    • Author(s)
      Ishikawa N, Mazur W, Toljamo T, Vuopala K, Rönty M, Horimasu Y, Kohno N, Kinnula VL.
    • Journal Title

      BMC Pulm Med

      Volume: 11 Issue: 1 Pages: 22-22

    • DOI

      10.1186/1471-2466-11-22

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Levels of Surfactant proteins A and D, and KL-6 are elevated in the induced sputum of COPD ; a sequential sputum analysis2011

    • Author(s)
      Ishikawa N, et al
    • Journal Title

      Respiration

      Volume: 82 Issue: 1 Pages: 10-18

    • DOI

      10.1159/000324539

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] KL-6 concentration in pulmonary epithelial lining fluid is a useful prognostic indicator in patients with acute respiratory distress syndrome.2011

    • Author(s)
      Kondo T, Hattori N, Ishikawa N, Murai H, Haruta Y, Hirohashi N, Tanigawa K, Kohno N.
    • Journal Title

      Respir Res

      Volume: 12 Issue: 1 Pages: 32-32

    • DOI

      10.1186/1465-9921-12-32

    • NAID

      120003692470

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Mucins carrying selectin ligands as predictive biomarkers of disseminated intravascular coagulation complication in ARDS2011

    • Author(s)
      Nakashima T, et al
    • Journal Title

      Chest

      Volume: 139 Issue: 2 Pages: 296-304

    • DOI

      10.1378/chest.09-3082

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] Antitumor effect of a humanized antibody against aminopeptidase N in mouse tumor models2013

    • Author(s)
      Akita S, Hattori N, Ishikawa N, Fujitaka K, Haruta Y, Murai H, Kohno N
    • Organizer
      18th congress of the Asian Pacific Society of Respirology
    • Place of Presentation
      横浜
    • Year and Date
      2013-11-11
    • Related Report
      2013 Final Research Report
  • [Presentation] Discovery and clinical application of KL-6, a circulating biomarker for interstitial lung diseases2013

    • Author(s)
      Kohno N
    • Organizer
      2013 European Respiratory Society Annual Congress
    • Place of Presentation
      Barcelona Spain
    • Year and Date
      2013-09-07
    • Related Report
      2013 Final Research Report
  • [Presentation] Discovery and clinical application of KL-6, a circulating biomarker for interstitial lung diseases.2013

    • Author(s)
      Kohno N.
    • Organizer
      2013 European Respiratory Society Annual Congress
    • Place of Presentation
      バルセロナ、スペイン
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Gene expression profiles of chronic hypersensitivity pneumonitis: Molecular signatures of pulmonary fibrosis.2013

    • Author(s)
      Ishikawa N, Taniwaki M, Horimasu Y, Iwamoto H, Ohshimo S, Fujitaka K, Haruta Y, Murai H, Hattori N, Kohno N.
    • Organizer
      American Thoracic society international Conference 2013
    • Place of Presentation
      フィラデルフィア、アメリカ
    • Related Report
      2013 Annual Research Report
  • [Presentation] Ethnic difference in the incidence of acute exacerbation in idiopathic pulmonary fibrosis2013

    • Author(s)
      Ohshimo S, Ishikawa N, Horimasu Y, Fujitaka K, Haruta Y, Murai H, Hattori N, Bonella F, Kohno N, Guzman J, Costabel U.
    • Organizer
      American Thoracic society international Conference 2013
    • Place of Presentation
      フィラデルフィア、アメリカ
    • Related Report
      2013 Annual Research Report
  • [Presentation] Difference of serum SP-D levels between German and Japanese are associated with SFTP gene polymorphisms.2013

    • Author(s)
      Horimasu Y, Hattori N, Ishikawa N, Tanaka S, Bonella F, Ohshimo S, Guzman J, Costabel U, KohnoN.
    • Organizer
      American Thoracic society international Conference 2013
    • Place of Presentation
      フィラデルフィア、アメリカ
    • Related Report
      2013 Annual Research Report
  • [Presentation] KL-6, SP-D and MMP-7 as serum biomarkers for early detection of interstitial lung diseases.2013

    • Author(s)
      Ishikawa N, Yoshioka K, Horimasu Y, Iwamoto H, Ohshimo S, Hattori N, Kohno N.
    • Organizer
      2013 European Respiratory Society Annual Congress
    • Place of Presentation
      バルセロナ、スペイン
    • Related Report
      2013 Annual Research Report
  • [Presentation] MUC1 gene polymorphisms are associated with serum KL-6 levels and pulmonary dysfunction in pulmonary alveolar proteinosis.2013

    • Author(s)
      Ohshimo S, Horimasu Y, Bonella F, Ishikawa N, Fujitaka K, Haruta Y, Murai H, Hirohashi N, Hattori N, Tanigawa K, KohnoN, Guzman J, Costabel U.
    • Organizer
      2013 European Respiratory Society Annual Congress
    • Place of Presentation
      バルセロナ、スペイン
    • Related Report
      2013 Annual Research Report
  • [Presentation] KL-6 and CCL18 as predictors for acute exacerbation in idiopathic pulmonary fibrosis.2012

    • Author(s)
      Ohshimo S , Horimasu Y, Bonella F, Ishikawa N, Hattori N, Tanigawa K, Kohno N, Guzman J, Costabel U.
    • Organizer
      American Thoracic Society International Conference
    • Place of Presentation
      San Francisco(アメリカ.)
    • Related Report
      2012 Research-status Report
  • [Presentation] Different serum cut-off values of KL-6 for German and Japanese to diagnose iIdiopathic interstitial pneumonias are related to different Mucin-1 gene polymorphisms.2012

    • Author(s)
      Horimasu Y, Hattori N, Ishikawa N, Tanaka S, Yoshioka K, Bonella F, Ohshimo S, Guzman J, Kohno N, Costabel U.
    • Organizer
      American Thoracic Society International Conference
    • Place of Presentation
      San Francisco(アメリカ.)
    • Related Report
      2012 Research-status Report
  • [Presentation] Different cutoff values of serum SP-D for German and Japanese to diagnose idiopathic interstitial pneumonias are related to different SFTPD gene polymorphisms.2012

    • Author(s)
      Horimasu Y, Hattori N, Ishikawa N, Tanaka S, Yoshioka K, Bonella F, Ohshimo S, Guzman J, Costabel U, Kohno N.
    • Organizer
      European Respiratory Society Annual Congress
    • Place of Presentation
      Vienna(オーストリア)
    • Related Report
      2012 Research-status Report
  • [Presentation] KL-6 is a useful serum biomarker for early detection of interstitial lung disease.2012

    • Author(s)
      Kohno N, Ishikawa N, Deguchi N, Iwamoto H, Ohshimo S, Fujitaka K, Haruta Y, Murai H, Hattori N.
    • Organizer
      European Respiratory Society Annual Congress
    • Place of Presentation
      Vienna(オーストリア)
    • Related Report
      2012 Research-status Report
  • [Presentation] Gene expression profiles of idiopathic interstitial pneumonias: Identification of disease-specific diagnostic markers and molecular therapeutic targets.2012

    • Author(s)
      Ishikawa N, Hattori N, Taniwaki M, Horimasu Y, Matsuda T, Kohno N.
    • Organizer
      European Respiratory Society Annual Congress
    • Place of Presentation
      Vienna(オーストリア)
    • Related Report
      2012 Research-status Report
  • [Presentation] Krebs von den Lungen-6 (KL-6) is a prognostic biomarker in patients with surgically resected nonsmall cell lung cancer2011

    • Author(s)
      Tanaka S, Ishikawa N, Hattori N, Hamada H, Yokoyama A, Kohno N
    • Organizer
      2011 European Respiratory Society Annual Congress
    • Place of Presentation
      Amsterdam, Netherlands
    • Year and Date
      2011-09-24
    • Related Report
      2013 Final Research Report
  • [Presentation] Serum KL-6 is a useful diagnostic and prognostic biomarker in idiopathic interstitial pneumonia in German patients.2011

    • Author(s)
      Horimasu Y, Ohshimo S, Ishikawa N, Tanaka S, Yoshioka K, Bonella F, Hattori N, Kohno N, Guzman J, Costabel U.
    • Organizer
      American Thoracic Society International Conference
    • Place of Presentation
      Denver, U.S.A.
    • Related Report
      2011 Research-status Report
  • [Presentation] Krebs von den lungen-6 (KL-6) is a useful biomarker for life threatening EGFR-TKI related interstitial lung disease2011

    • Author(s)
      Kawase S, Hattori N, Ishikawa N, Horimasu Y, Furonaka O, Isobe T, Hamada H, Yokoyama A, Kohno N
    • Organizer
      American Thoracic Society International Conference
    • Place of Presentation
      Denver, U.S.A.
    • Related Report
      2011 Research-status Report
  • [Presentation] nterferon (-alpha, -beta, and -omega) receptor 2 (IFNAR2) is a prognostic biomarker for lung cancer.2011

    • Author(s)
      Ishikawa N, Hattori N, Tanaka S, Horimasu Y, Yokoyama A, Kohno N.
    • Organizer
      European Respiratory Society Annual Congress
    • Place of Presentation
      Amsterdam, Netherlands
    • Related Report
      2011 Research-status Report
  • [Presentation] Serum SP-A and SP-D: different cutoff values for German and Japanese patients to diagnose idiopathic interstitial pneumonia.2011

    • Author(s)
      Horimasu Y, Hattori N, Ishikawa N, Tanaka S, Yoshioka K, Ohshimo S, Bonella F, Guzman J, Costabel U, Kohno N.
    • Organizer
      European Respiratory Society Annual Congress
    • Place of Presentation
      Amsterdam, Netherlands
    • Related Report
      2011 Research-status Report
  • [Presentation] Reliability of ATS/ERS criteria for the diagnosis of idiopathic pulmonary fibrosis.2011

    • Author(s)
      Ohshimo S, Bonella F, Tanigawa K, Kohno N, Guzman J, Costabel U.
    • Organizer
      European Respiratory Society Annual Congress
    • Place of Presentation
      Amsterdam, Netherlands
    • Related Report
      2011 Research-status Report
  • [Book] がんの浸潤・転移2011

    • Author(s)
      石川暢久,河野修興
    • Total Pages
      241
    • Publisher
      南山堂
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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