Challenge for the identification of ALS-related viruses
| Project/Area Number |
23659453
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Neurology
|
|---|
| Research Institution | Osaka University |
|---|
Principal Investigator |
KAWAHARA Yukio 大阪大学, 大学院・医学系研究科, 特任准教授 (常勤) (80542563)
|
|---|
| Project Period (FY) |
2011 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Keywords | 神経分子病態学 / ALS / ウイルス / 神経変性疾患 / 脳神経疾患 / RNA / 筋萎縮性側索硬化症 / 痴呆 / 遺伝子 |
|---|
| Research Abstract |
The aim of this study is to identify RNAs derived from viruses as a causative origin from the autopsied spinal cord of ALS patients. We have successfully established the method to create a cDNA library from RNAs extracted from cultured cells after removal of ribosomal RNAs. We had obtained one autopsied spinal cord of ALS patients. Then, we extracted RNAs from this samples, but it was degraded more severely than expected. We tried to create a cDNA library from this degraded RNAs, but failed in. Since we could establish the method to create cDNA library for deep sequencing, it is promising to analyze whether viral RNAs are involved in or not in ALS samples if we can obtain another samples.
|
Report
(3 results)
Research Products
(22 results)
-
-
-
-
[Journal Article] A Subset of Circulating MicroRNAs are Predictive for Cardiac Death After Discharge for Acute Myocardial Infarction2012
Author(s)
Matsumoto S, Sakata Y, Nakatani D, Suna S, Mizuno H, Shimizu M, Usami M, Sasaki T, Sato H, Kawahara Y, Hamasaki T, Nanto S, Hori M and Komuro I.
-
Journal Title
Biochemical and Biophysical Research Communications
Volume: 427(2)
Pages: 280-284
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] small RNAs
Author(s)
KAWAHARA Yukio
Organizer
第７回日仏先端シンポジウム
Place of Presentation
ロイヤルオークホテル (滋賀)
Related Report
Invited
-
-
-
