| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Research Abstract |
The aim of this study is to identify RNAs derived from viruses as a causative origin from the autopsied spinal cord of ALS patients. We have successfully established the method to create a cDNA library from RNAs extracted from cultured cells after removal of ribosomal RNAs. We had obtained one autopsied spinal cord of ALS patients. Then, we extracted RNAs from this samples, but it was degraded more severely than expected. We tried to create a cDNA library from this degraded RNAs, but failed in. Since we could establish the method to create cDNA library for deep sequencing, it is promising to analyze whether viral RNAs are involved in or not in ALS samples if we can obtain another samples.
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