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Analysis of process from ER stress to cell death in an animal model for disease

Research Project

Project/Area Number 23659475
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Endocrinology
Research InstitutionNagoya University

Principal Investigator

OISO Yutaka  名古屋大学, 医学(系)研究科(研究院), 教授 (40203707)

Co-Investigator(Kenkyū-buntansha) ARIMA Hiroshi  名古屋大学, 大学院医学系研究科, 准教授 (50422770)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywordsバゾプレシン / 小胞体ストレス / オートファギー / 細胞死
Research Abstract

Familial neurohypophysial diabetes insipidus (FNDI) model mice were subjected to intermittent water deprivation in order to promote the phenotypes. Electron microscopic analyses demonstrated that, while aggregates are confined to a certain compartment of the ER in the AVP neurons of FNDI mice with water access ad libitum, they were scattered throughout the dilated ER lumen in the FNDI mice subjected to water deprivation for 4 weeks. It is also demonstrated that phagophores, the autophagosome precursors, emerged in the vicinity of aggregates and engulfed the ER containing scattered aggregates. The cytoplasm of AVP neurons in FNDI mice was occupied with vacuoles in the mice subjected to water deprivation for 12 weeks, when 30-40% of AVP neurons are lost. Our data thus demonstrated that autophagy was induced in the AVP neurons subjected to ER stress in FNDI mice.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (15 results)

All 2014 2013 2012 2011

All Journal Article (2 results) (of which Peer Reviewed: 1 results) Presentation (13 results) (of which Invited: 2 results)

  • [Journal Article] Arginine vasopressin neuronal loss results from autophagy-associated cell death in a mouse model for familial neurohypophysial diabetes insipidus2014

    • Author(s)
      Hagiwara D, Arima H, Morishita Y, Wenjun L, Azuma Y, Ito Y, Suga H, Goto M, Banno R, Sugimura Y, Shiota A, Asai N, Takahashi M, Oiso Y
    • Journal Title

      Cell Death Dis

      Volume: 27;5

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] BiP mRNA expression is upregulated by dehydration in vasopressin neurons in the hypothalamus in mice2012

    • Author(s)
      Hagiwara D, Arima H, Morishita Y, Goto M, Banno R, Sugimura Y, Oiso Y
    • Journal Title

      Peptides

      Volume: 33(2) Pages: 346-50

    • Related Report
      2011 Research-status Report
  • [Presentation] バゾプレシンニューロンはER ファジーと選択的オートファジーにより細胞死に至る―家族性中枢性尿崩症モデルマウスを用いた検討―2014

    • Author(s)
      萩原大輔、有馬寛、盧文君、東慶成、大磯ユタカ
    • Organizer
      第87回日本内分泌学会学術総会
    • Place of Presentation
      福岡国際会議場・福岡サンパレス(福岡市)
    • Related Report
      2013 Final Research Report
  • [Presentation] バゾプレシンニューロンと小胞体ストレス2014

    • Author(s)
      有馬寛
    • Organizer
      第87回日本内分泌学会学術総会
    • Place of Presentation
      福岡国際会議場・福岡サンパレス(福岡市)
    • Related Report
      2013 Final Research Report
  • [Presentation] バゾプレシンニューロンはERファジーと選択的オートファジーにより細胞死に至る―家族性中枢性尿崩症モデルマウスを用いた検討―2014

    • Author(s)
      萩原大輔、有馬寛、盧文君、東慶成、大磯ユタカ
    • Organizer
      第87回日本内分泌学会学術総会
    • Place of Presentation
      福岡国際会議場(福岡)
    • Related Report
      2013 Annual Research Report
  • [Presentation] バゾプレシンニューロンと小胞体ストレス2014

    • Author(s)
      有馬寛
    • Organizer
      第87回日本内分泌学会学術総会
    • Place of Presentation
      福岡国際会議場(福岡)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Aggregate formation in endoplasmic reticulum of vasopressin neurons in familial diabetes insipidus model mice2013

    • Author(s)
      Arima H
    • Organizer
      10^<th> World Congress on Neurohypophysial Hormones
    • Place of Presentation
      Bristol, UK
    • Related Report
      2013 Final Research Report
  • [Presentation] ER-associated complex の形成はオートファギー細胞死からバゾプレシンニューロンを保護する2013

    • Author(s)
      萩原大輔、有馬寛、盧文君、東慶成、森下啓明、大磯ユタカ
    • Organizer
      第86回日本内分泌学会学術総会
    • Place of Presentation
      仙台国際センター(仙台市)
    • Related Report
      2013 Final Research Report
  • [Presentation] ER-associated complexの形成はオートファギー細胞死からバゾプレシンニューロンを保護する2013

    • Author(s)
      萩原大輔、有馬寛、盧文君、東慶成、森下啓明、大磯ユタカ
    • Organizer
      第86回日本内分泌学会学術総会
    • Place of Presentation
      仙台国際センター(仙台)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Aggregate formation in endoplasmic reticulum of vasopressin neurons in familial diabetes insipidus model mice2013

    • Author(s)
      Arima H
    • Organizer
      10th World Congress on Neurohypophysial Hormones
    • Place of Presentation
      Bristol (UK)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 家族性中枢性尿崩症モデルマウスにおける脱水負荷は小胞体内凝集体形成を阻害しバゾプレシンンユーロンの細胞死を促進する2012

    • Author(s)
      萩原大輔、有馬寛、森下啓明、後藤資実、坂野僚一、椙村益久、大磯ユタカ
    • Organizer
      第85回日本内分泌学会学術総会
    • Place of Presentation
      名古屋国際会議場(名古屋)
    • Related Report
      2012 Research-status Report
  • [Presentation] 家族性中枢性尿崩症ではオートファジーによりバゾプレシンニューロンの細胞死が生じる2012

    • Author(s)
      萩原大輔,有馬寛,盧文君,東慶成,森下啓明,大磯ユタカ
    • Organizer
      第39回日本神経内分泌学会学術集会
    • Place of Presentation
      北九州国際会議場(北九州)
    • Related Report
      2012 Research-status Report
  • [Presentation] Aggregate formation in the endoplasmic reticulum of vasopressin neurons alleviates endoplasmic reticulum stress and protects against cell death in a mouse model for familial neurohypophysial diabetes insipidu2012

    • Author(s)
      Hagiwara D , Arima H, Azuma Y, Morishta Y, Oiso Y
    • Organizer
      Neuroscience 2012
    • Place of Presentation
      New Orleans (USA)
    • Related Report
      2012 Research-status Report
  • [Presentation] 家族性中枢性尿崩症における小胞体凝集体の意義-モデルマウスを用いた検討2012

    • Author(s)
      萩原大輔、有馬寛、森下啓明、大磯ユタカ
    • Organizer
      第22回バソプレシン研究会
    • Place of Presentation
      東京
    • Related Report
      2011 Research-status Report
  • [Presentation] 脱水負荷はバゾプレシンニューロンにおけるBiP mRNA発現を増強する2011

    • Author(s)
      萩原大輔、有馬寛、森下啓明、大磯ユタカ
    • Organizer
      第38回日本神経内分泌学会学術集会
    • Place of Presentation
      東京
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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