Development of the multiresonance magnetic resonance imaging technique

• Project/Area Number: 23659586
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Radiation science
• Research Institution: Kyoto University
• Principal Investigator: TOCHIO Hidehito 京都大学, 大学院・工学研究科, 准教授 (70336593)
• Project Period (FY): 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2011: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
• Keywords: MRI / NMR / 多重共鳴NMR

Research Abstract

Multiple-resonance NMR is routinely employed in structure analysis of proteins. In this study, application of the multiple-resonance principle to Magnetic Resonance Imaging(MRI) was examined. As a result, triple-resonance MRI has been demonstrated to be feasible at least on aqueous solution sample. In addition, a series of 13C polymers have been shown to be an excellent molecular probe for 1H-{13C} double resonance MRI in tumor-bearing mice.

Research Products

• [Journal Article] Substrate/Product-targeted NMR monitoring of pyrimidine catabolism and its inhibition by a clinical drug (2012)
  • Author(s): Yamada H, Mizusawa K, Igarashi R, Tochio H, Shirakawa M, Tabata Y, Kimura Y, Kondo T, Aoyama Y, Sando S
  • Journal Title: ACS Chem Biol Volume: 7巻 Pages: 535-42
  • Peer Reviewed
• [Journal Article] 磁気共鳴を使った真核細胞内タンパク質の構造・機能解析 (2012)
  • Author(s): 杤尾豪人、白川昌宏
  • Journal Title: 薬学雑誌 Volume: 132巻 Pages: 185-193
  • NAID: 130001872007
• [Journal Article] NMR analysis of Lys63-linked polyubiquitin recognition by the tandem ubiquitin-interacting motifs of Rap80 (2012)
  • Author(s): Sekiyama N, Jee J, Isogai S, Akagi K, Huang TH, Ariyoshi M, Tochio H, Shirakawa M
  • Journal Title: J Biomol NMR Volume: 52巻 Pages: 339-350
  • Peer Reviewed
• [Journal Article] NMR analysis of Lys63-linked polyubiquitin recognition by the tandem ubiquitin-interacting motifs of Rap80. (2012)
  • Author(s): Sekiyama N, Jee J, Isogai S, Akagi K, Huang TH, Ariyoshi M, Tochio H, Shirakawa M.
  • Journal Title: J Biomol NMR. Volume: 52 Issue: 4 Pages: 339-350
  • DOI: 10.1007/s10858-012-9614-9
  • Peer Reviewed
• [Journal Article] Crystal structure of the UBA domain of p62 and its interaction with ubiquitin (2011)
  • Author(s): Isogai, S., Morimoto, D., Arita, K., Unzai, S., Tenno, T., Hasegawa, J., Sou, Y.S., Komatsu, M., Tanaka, K., Shirakawa, M., Tochio, H.
  • Journal Title: J Biol Chem. Volume: 286 Issue: 36 Pages: 31864-318674
  • DOI: 10.1074/jbc.m111.259630
  • Peer Reviewed
• [Journal Article] Substrate/Product-targeted NMR monitoring of pyrimidine catabolism and its inhibition by a clinical drug. (2011)
  • Author(s): Yamada H, Mizusawa K, Igarashi R, Tochio H, Shirakawa M, Tabata Y, Kimura Y, Kondo T, Aoyama Y, Sando S.
  • Journal Title: ACS Chem Biol. Volume: 7 Issue: 3 Pages: 535-542
  • DOI: 10.1021/cb2003972
  • Peer Reviewed
• [Presentation] Magnetic resonance methods for analyses of structure and dynamics of intracellular proteins and cells (2011)
  • Author(s): 杤尾豪人
  • Organizer: 第49回生物物理学会年会
  • Place of Presentation: 姫路
• [Presentation] Analysis of proteins inside human cultured cells by NMR (2011)
  • Author(s): 杤尾豪人
  • Organizer: The 4th Asia Pacific NMR symposium
  • Place of Presentation: Beijing、中国
• [Presentation] Magnetic resonance methods for analyses of structure and dynamics of intracellular proteins and cells (2011)
  • Author(s): 杤尾豪人
  • Organizer: 第49回日本生物物理学会年会
  • Place of Presentation: 姫路
• [Presentation] Analysis of proteins inside human cultured cells by NMR (2011)
  • Author(s): Hidehito Tochio
  • Organizer: The 4th Asia Pacific NMR symposium
  • Place of Presentation: Beijing, China
• [Presentation] Structural basis for receptor recognition of interleukin 18 (2011)
  • Author(s): 堤尚孝、木村豪、大西秀典、加藤善一郎、近藤直実
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Book] 「蛍光イメージング/MRIプローブの開発」第12章核磁気共鳴を利用した生体計測 (2011)
  • Author(s): 杤尾豪人、白川昌宏
  • Publisher: シーエムシー出版