High-throughput screening for peptides that inhibit the interaction or MDM4 with p53
Project/Area Number |
23659658
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Digestive surgery
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Research Institution | Sapporo Medical University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
FURUHATA Tomohisa 札幌医科大学, 医学部, 准教授 (80359992)
|
Project Period (FY) |
2011 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | p53 / MDM4 / 癌 / 分子標的 |
Research Abstract |
It is currently of great midical interest to inhibit specific protein-protein interactions between MDM4 and p53. Here, we developed a method using T7 phage display to screen for candidate inhibitors of MDM4-p53 interactions. In primary screening , we identified novel peptide motifs that inhibit the interaction of MDM4 with p53. Then, we designed a number of peptides that are predicted to have higher affinity to MDM4, and identified potential peptides that inhibit the interaction between MDM4 and p53 with higher affinity.
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Report
(3 results)
Research Products
(30 results)
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Author(s)
Shimizu T, Suzuki H, Nojima M, Kitamura H, Yamamoto E, Maruyama R, Ashida M, Hatahira T, Kai M, Masumori N, Tokino T, Imai K, Tsukamoto T, Toyota M
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Journal Title
Eur Urol
Volume: 63
Issue: 6
Pages: 1091-1100
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[Journal Article] Genomic vulnerability to LINE-1 hypermethylation is a potential determinant of the clinicogenetic features of multiple myeloma.2012
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Aoki Y, Nojima M, Suzuki H, Yasui H, Maruyama R, Yamamoto E, Ashida M, Itagaki M, Asaoku H, Ikeda H, Hayashi T, Imai K, Mori M, Tokino T, Ishida T, Toyota M, Shinomura Y
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Journal Title
Genome Medicine
Volume: (印刷中)
Issue: 12
Pages: 101-101
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[Journal Article] CLCA2, a target of the p53 family, negatively regulates cancer cell migration and invasion2012
Author(s)
Sasaki Y, Sugisaka J, Maruyama R, Sugisaka J, Tamura M, Sugisaka J, Suzuki H, Idogawa M, Shinomura Y, Tokino T
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Journal Title
Cancer Biol Ther
Volume: 13
Issue: 14
Pages: 1512-1521
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[Journal Article] A novel approach to cancer treatment using structural hybrids of the p53 gene family.2012
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Sasaki Y, Oshima Y, Koyama R, Tamura M, Kashima L, Idogawa M, Yamashita T, Toyota M, Imai K, Shinomura Y, Tokino T.
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Journal Title
Cancer Gene Ther
Volume: 19
Issue: 11
Pages: 749-56
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[Journal Article] CHFR regulates the mitotic checkpoint by targeting PARP-1 for ubiquitination and degradation2012
Author(s)
Kashima, L., Idogawa, M., Mita, H., Shitashige, M., Yamada, T., Ogi, K., Suzuki, H., Toyota, M., Ariga, H., Sasaki, Y., Tokino, T.
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Journal Title
J. Biol. Chem
Volume: 287
Issue: 16
Pages: 12975-12984
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[Journal Article] Heat shock enhances the expression of cytotoxic granule proteins and augments the activities of tumor-associated antigen-specific cytotoxic T lymphocytes2012
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Takahashi A, Torigoe T, Tamura Y, Kanaseki T, Tsukahara T, Sasaki Y, Kameshima H, Tsuruma T, Hirata K, Tokino T, Hirohashi Y, Sato N
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Cell Stress Chaperones
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[Journal Article] Genome-wide analysis of DNA methylation identifies novel cancer-related genes in hepatocellular carcinoma2012
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Shitani M, Sasaki S, Akutsu N, Takagi H, Suzuki H, Nojima M, Yamamoto H, Tokino T, Hirata K, Imai K, Toyota M, Shinomura Y
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Tumour Biol
Volume: 33
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[Journal Article] p53 negatively regulates the hepatoma growth factor HDGF2011
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Sasaki Y, Negishi H, Idogawa M, Yokota I, Koyama R, Kusano M, Suzuki H, Fujita M, Maruyama R, Toyota M, Saito T, Tokino T
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Journal Title
Cancer Res
Volume: 71
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