| Project/Area Number |
23659659
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Digestive surgery
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| Research Institution | Tokyo Women's Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
ARIIZUMI Syunnichi 東京女子医科大学, 医学部, 講師 (40277158)
OHTA Takehiro 東京女子医科大学, 医学部, 講師 (70203792)
SHODA Junichi 筑波大学, 医学医療系, 教授 (90241827)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 肝内胆管癌 / バイオマーカー / 糖鎖医学 / レクチン / 胆道癌モデル |
|---|
| Research Abstract |
As a result of tissue-targeted lectin microarray analysis, we found that Wisteria floribunda agglutinin (WFA) is the best probe to differentiate biliary tract cancer lesions from normal bile duct epithelia (BDE) (P < 0.0001). The subsequent histochemical study confirmed cancer-specific WFA staining on the tissue specimens. Furthermore, the results of the experiments strongly implied that sialylated MUC1 is a candidate glycoprotein that carries biliary tract cancer-associated, WFA-reactive glycans. Therefore, we developed a new WFA-MY.1E12 sandwich detection ELISA that is a highly sensitive diagnostic method for biliary tract cancers using bile specimens. The diagnostic sensitivity of WFA-MY.1E12 for the detection of biliary tract cancers were found to be superior to that of CA19-9
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