| Project/Area Number |
23659670
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Thoracic surgery
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| Research Institution | Osaka University |
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Principal Investigator |
TORIKAI Kei 大阪大学, 医学系研究科, 助教 (70364792)
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| Co-Investigator(Kenkyū-buntansha) |
SAWA Yosiki 大阪大学, 大学院・医学系研究科, 教授 (00243220)
KURATANI Toru 大阪大学, 大学院・医学系研究科, 寄附講座 教授 (90448035)
SAKAGUCHI Taichi 大阪大学, 大学院・医学系研究科, 寄附講座 教授 (10467574)
SHIRAKAWA Yukitoshi 大阪大学, 大学院・医学系研究科, 寄附講座 教授 (20457013)
SHIMAMURA Kazuo 大阪大学, 医学部附属病院, 医員 (10507205)
KIN Keiwa 大阪大学, 大学院・医学系研究科, 寄附講座助教 (70532985)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | miRNA / 大動脈瘤 / AAA / 動脈硬化 |
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| Research Abstract |
This study examined tissue- and plasma-microRNAs specifically associated with AAA. AAA and normal wall tissues were sampled from patients undergoing AAA repair and aortic valve replacement surgery, respectively. MicroRNA expression was assessed by high-throughput microRNA arrays and validated by real-time PCR for individual microRNAs that showed significant expression differences in the initial screening. MicroRNAs related to fibrosis (miR-29b), inflammation (miR-124a, miR-146a, miR-155 and miR-223), and endothelium (miR-126, let-7 family members and miR-21) were significantly upregulated in AAA tissue. Expression levels of monocyte chemoattractant protein-1 and miR-124a, 146a and 223, tumor necrosis factor-α and miR-126 and 223, and transforming growth factor-β and miR-146a showed a significant negative correlation. Expression of microRNAs, such as miR-29b, miR-124a, miR-155 and miR-223, upregulated in AAA tissue was significantly reduced in plasma of AAA patients compared to healthy controls and coronary artery disease patients.
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