• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Effect of abnormalities in asymmetric cell divisions of neural stem cells on brain tumor initiation

Research Project

Project/Area Number 23659690
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Cerebral neurosurgery
Research InstitutionKochi University

Principal Investigator

SHIMIZU Keiji  高知大学, 教育研究部医療学系, 教授 (70116044)

Co-Investigator(Kenkyū-buntansha) YAWATA Toshio  高知大学, 教育研究部・医療学系, 助教 (40380323)
HIGASHI Yoichiro  高知大学, 教育研究部・医療学系, 助教 (80380062)
Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords脳腫瘍 / エピジェネティクス / 分化 / 悪性脳腫瘍 / 幹細胞 / 非対称分裂
Research Abstract

A method for distinguishing a stem cell from a differentiated cell using stemness gene’s promoter was developed to examine the abnormal asymmetrical cell division in malignant glioma cell cultures and tissues. By flow cytometric analysis using stem and precursor cell markers, it was revealed that glioma stem and precursor cells over-expressed MDR1 (multiple drug resistance 1) and exhibited low susceptibility to DNA damage by anti-cancer drugs.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (41 results)

All 2013 2012 2011 Other

All Journal Article (11 results) (of which Peer Reviewed: 11 results) Presentation (30 results) (of which Invited: 2 results)

  • [Journal Article] Regulatory effects of Spirulina complex polysaccharides on growth of murine RSV-M gliomas cells through toll-like receptor 42013

    • Author(s)
      Kawanishi Y, Tominaga A, Okuyama H, Fukuoka S, Taguchi T, Kusumoto Y, Yawata T, Fujimoto Y, Ono S, Shimizu K
    • Journal Title

      Microbiol Immunol

      Volume: 57 Pages: 63-73

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Attenuation of axonal injury and oxidative stress by edaravone protects against cognitive impairments after traumatic brain injury2013

    • Author(s)
      Ohta M, Higashi Y, Yawata T, Kitahara M, Nobumoto A, Ishida R, Tsuda M, Fujimoto Y, Shimizu K
    • Journal Title

      Brain Res

      Volume: 1490 Pages: 184-192

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Regulatory effects of Spirulina complex polysaccharides on growth of murine RSV-M gliomas cells through toll-like receptor 4.2013

    • Author(s)
      Kawanishi Y
    • Journal Title

      Microbiology and Immunology

      Volume: 57 Issue: 1 Pages: 63-73

    • DOI

      10.1111/1348-0421.12001

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Attenuation of axonal injury and oxidative stress by edaravone protects against cognitive impairments after traumatic brain injury.2013

    • Author(s)
      Ohta M
    • Journal Title

      Brain Research

      Volume: 1490 Pages: 184-192

    • DOI

      10.1016/j.brainres.2012.09.011

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Stereoscopic virtual realistic surgical simulation in intracranial aneurysms2012

    • Author(s)
      Nakabayashi H, Shimizu K
    • Journal Title

      Neurology India

      Volume: 60 Pages: 191-197

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Stereoscopic virtual realistic surgical simulation in intracranial aneurysms.2012

    • Author(s)
      Nakabayashi H
    • Journal Title

      Neurology India

      Volume: 60 Issue: 2 Pages: 191-197

    • DOI

      10.4103/0028-3886.96399

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Involvement of Akt/NF-κB pathway in antitumor effects of parthenolide on glioblastoma cells in vitro and in vivo.2012

    • Author(s)
      Nakabayashi H
    • Journal Title

      BMC Cancer

      Volume: 12 Issue: 1 Pages: 453-453

    • DOI

      10.1186/1471-2407-12-453

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification and functional characterization of glioma-specific promoters and their application in suicide gene therapy2011

    • Author(s)
      Yawata T, Maeda Y, Okiku M, Ishida E, Ikenaka K, Shimizu K
    • Journal Title

      J Neurooncol

      Volume: 04 Pages: 497-507

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification and functional characterization of glioma-specific promoters and their application in suicide gene therapy2011

    • Author(s)
      Yawata T, Maeda Y, Ohkiku M, et al
    • Journal Title

      Journal of Neuro-oncology

      Volume: 104 Issue: 2 Pages: 497-507

    • DOI

      10.1007/s11060-010-0522-0

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] HA1077, a Rho kinase inhibitor, suppresses glioma-induced angiogenesis by targeting the Rho-ROCK and the mitogen-activated protein kinase kinase/extracellular signal-regulated kinase (MEK/ERK) signal pathways.2011

    • Author(s)
      Nakabayashi H
    • Journal Title

      Cancer Sci

      Volume: 102(2) Issue: 2 Pages: 393-399

    • DOI

      10.1111/j.1349-7006.2010.01794.x

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Gene therapy strategies for treating brain tumors: Retroviruses are still good candidates for therapeutic vectors

    • Author(s)
      Yawata T, Shimizu K
    • Journal Title

      Open J Genet.

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Presentation] Development of a high-titer retrovirus production system for glioma gene therapy2013

    • Author(s)
      Shimizu K
    • Organizer
      Joint Neurosurgical Convention 2013
    • Place of Presentation
      Waikoloa, Hawaii, U.S.A
    • Related Report
      2012 Final Research Report
  • [Presentation] Development of a high-titer retrovirus production system for glioma gene therapy.2013

    • Author(s)
      Shimizu K
    • Organizer
      Joint Neurosurgical Convention 2013
    • Place of Presentation
      Hilton Waikoloa Village (U.S.A.)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Towards a clinical trial of gene therapy for malignant glioma using highly concentrated retrovirus solution2012

    • Author(s)
      Shimizu K
    • Organizer
      ESGCT 20th Anniversary Congress in Collaboration with the SFTCG
    • Place of Presentation
      Versailles, France
    • Related Report
      2012 Final Research Report
  • [Presentation] 頭部外傷後の軸索損傷と認知機能障害に対するエダラボンの効果の解析2012

    • Author(s)
      大田 学
    • Organizer
      第13回日本分子脳神経外科学会
    • Place of Presentation
      熊本
    • Related Report
      2012 Final Research Report
  • [Presentation] Multidrug-resistance (MDR-1) gene expression and susceptibility of immature glioma cells to drug-induced DNA damage2012

    • Author(s)
      八幡 俊男
    • Organizer
      第71回日本癌学会学術総会
    • Place of Presentation
      北海道
    • Related Report
      2012 Final Research Report
  • [Presentation] エダラボンは頭部外傷による軸索損傷を阻止し、記憶障害を改善する2012

    • Author(s)
      東 洋一郎
    • Organizer
      第35回日本神経科学大会
    • Place of Presentation
      愛知
    • Related Report
      2012 Final Research Report
  • [Presentation] Spirulina CPSを用いた悪性神経膠腫に対する免疫療法の検討2012

    • Author(s)
      川西 裕
    • Organizer
      第16回日本がん免疫学会総会
    • Place of Presentation
      北海道
    • Related Report
      2012 Final Research Report
  • [Presentation] DNA障害を指標とした悪性グリオーマ幹細胞の抗癌剤感受性の検討2012

    • Author(s)
      八幡 俊男
    • Organizer
      第22回日本サイトメトリー学会学術集会
    • Place of Presentation
      大阪
    • Related Report
      2012 Final Research Report
  • [Presentation] Multidrug-resistance (MDR-1) gene expression in immature glioma cells2012

    • Author(s)
      Shimizu K
    • Organizer
      American Association for Cancer Research, Annual Meeting 2012
    • Place of Presentation
      Chicago, IL, U.S.A
    • Related Report
      2012 Final Research Report
  • [Presentation] Towards a clinical trial of gene therapy for malignant glioma using highly concentrated retrovirus solution.2012

    • Author(s)
      Shimizu K
    • Organizer
      ESGCT 20th Anniversary Congress in Collaboration with the SFTCG
    • Place of Presentation
      Palais des Congres (France)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Multidrug-resistance (MDR-1) gene expression in immature glioma cells.2012

    • Author(s)
      Shimizu K
    • Organizer
      American Association for Cancer Research, Annual Meeting 2012
    • Place of Presentation
      McCormick Place (U.S.A.)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 頭部外傷後の軸索損傷と認知機能障害に対するエダラボンの効果の解析2012

    • Author(s)
      大田 学
    • Organizer
      第13回日本分子脳神経外科学会
    • Place of Presentation
      熊本市国際交流会館(熊本)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Multidrug-resistance (MDR-1) gene expression and susceptibility of immature glioma cells to druginduced DNA damage.2012

    • Author(s)
      八幡 俊男
    • Organizer
      第71回日本癌学会学術総会
    • Place of Presentation
      ロイトン札幌、さっぽろ芸文館、札幌市教育文化会館(北海道)
    • Related Report
      2012 Annual Research Report
  • [Presentation] エダラボンは頭部外傷による軸索損傷を阻止し、記憶障害を改善する2012

    • Author(s)
      東 洋一郎
    • Organizer
      第35回日本神経科学大会
    • Place of Presentation
      名古屋国際会議場(愛知)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Spirulina CPSを用いた悪性神経膠腫に対する免疫療法の検討2012

    • Author(s)
      川西 裕
    • Organizer
      第16回日本がん免疫学会総会
    • Place of Presentation
      北海道大学学術交流会館(北海道)
    • Related Report
      2012 Annual Research Report
  • [Presentation] DNA障害を指標とした悪性グリオーマ幹細胞の抗癌剤感受性の検討2012

    • Author(s)
      八幡 俊男
    • Organizer
      第22回日本サイトメトリー学会学術集会
    • Place of Presentation
      千里ライフサイエンスセンター(大阪)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 悪性グリオーマの治療に対する新たな展開2012

    • Author(s)
      清水 惠司
    • Organizer
      第5回静岡県脳腫瘍研究会
    • Place of Presentation
      アクトシティー浜松(静岡)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 悪性グリオーマに対する新たな治療法を求めて2012

    • Author(s)
      清水 惠司
    • Organizer
      第69回山形脳神経外科懇話会
    • Place of Presentation
      山形国際ホテル(山形)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Brain tumor selective gene expression and therapy mediated by recombinant retroviruses containing the SSX4 promoter2011

    • Author(s)
      Shimizu K
    • Organizer
      ESGCT and BSGT Collaborative Congress 2011
    • Place of Presentation
      Brighton, U.K.
    • Related Report
      2012 Final Research Report
  • [Presentation] 悪性神経膠腫の MGMT発現評価に対する新たな試み2011

    • Author(s)
      川西 裕
    • Organizer
      (社)日本脳神経外科学会第70回学術総会
    • Place of Presentation
      神奈川
    • Related Report
      2012 Final Research Report
  • [Presentation] 未分化なグリオーマ細胞における薬剤耐性遺伝子の発現2011

    • Author(s)
      八幡 俊男
    • Organizer
      第70回日本癌学会学術集会
    • Place of Presentation
      愛知
    • Related Report
      2012 Final Research Report
  • [Presentation] てんかん発作による海馬歯状回神経新生異常に対する亜鉛投与の効果2011

    • Author(s)
      東 洋一郎
    • Organizer
      第34回日本神経科学大会
    • Place of Presentation
      神奈川
    • Related Report
      2012 Final Research Report
  • [Presentation] 悪性グリオーマ内在性の未分化細胞における薬剤耐性遺伝子の発現2011

    • Author(s)
      八幡 俊男
    • Organizer
      第21回日本サイトメトリー学会学術集会
    • Place of Presentation
      京都
    • Related Report
      2012 Final Research Report
  • [Presentation] PCR in situ hybridizationを用いた MGMT発現評価の試み2011

    • Author(s)
      川西 裕
    • Organizer
      第29回日本脳腫瘍病理学会学術集会
    • Place of Presentation
      東京
    • Related Report
      2012 Final Research Report
  • [Presentation] Transcriptional targeting of retrovirus vector through the SSX4 tumor-specific promoter2011

    • Author(s)
      Yawata T
    • Organizer
      American Society of Gene & Cell Therapy 14th Annual Meeting
    • Place of Presentation
      Seattle, WA, U.S.A.
    • Related Report
      2012 Final Research Report
  • [Presentation] Brain tumor selective gene expression and therapy mediated by recombinant retroviruses containing the SSX4 promoter.2011

    • Author(s)
      Shimizu K
    • Organizer
      ESGCT and BSGT Collaborative Congress 2011
    • Place of Presentation
      The Brighton Centre(U.K.)
    • Related Report
      2011 Research-status Report
  • [Presentation] 悪性グリオーマ未分化細胞における薬剤耐性遺伝子MDR1の発現.2011

    • Author(s)
      竹村 光広
    • Organizer
      (社)日本脳神経外科学会第70回学術総会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Related Report
      2011 Research-status Report
  • [Presentation] 未分化なグリオーマ細胞における薬剤耐性遺伝子の発現.2011

    • Author(s)
      八幡 俊男
    • Organizer
      第70回日本癌学会学術集会
    • Place of Presentation
      名古屋国際会議場(愛知県)
    • Related Report
      2011 Research-status Report
  • [Presentation] 悪性グリオーマ内在性の未分化細胞における薬剤耐性遺伝子の発現.2011

    • Author(s)
      八幡 俊男
    • Organizer
      第21回日本サイトメトリー学会学術集会
    • Place of Presentation
      京都国際会議場(京都府)
    • Related Report
      2011 Research-status Report
  • [Presentation] Transcriptional targeting of retrovirus vector through the SSX4 tumor-specific promoter.2011

    • Author(s)
      Yawata T
    • Organizer
      American Society of Gene & Cell Therapy 14th Annual Meeting
    • Place of Presentation
      Washington State Convention Center(U.S.A.)
    • Related Report
      2011 Research-status Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi