Stud aiming at elucidating the molecular mechanism behind spondylosis through establishing cre / lox P system in the field of intervertebral disc.

• Project/Area Number: 23659706
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: The University of Tokyo
• Principal Investigator: KAWAGUCHI Hiroshi 東京大学, 医学部附属病院, 准教授 (40282660)
• Co-Investigator(Kenkyū-buntansha): HARA Nobuhiro 東京大学, 医学部附属病院, 助教 (00422296) ; ONO Takashi 東京大学, 医学部附属病院, 特任助教 (00506248)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 脊椎脊髄病学 / 椎間板 / 変形性脊椎症 / 椎間板変性

Research Abstract

We established a new mouse model of spondylosis. In this model, we identified that annulus fibrosus cells exhibit hypertrophic change morphologically with expression of type 10 collagen. Also we succeeded in identifying a candidate gene which can be a marker of annulus fibrosus of intervertebral disc.

Research Products

• [Journal Article] Notch signaling in chondrocytes modulates endochondral ossification and osteoarthritis development (2013)
  • Author(s): Hosaka Y, Saito T, Sugita S, Hikata T, Kobayashi H, Fukai A, Taniguchi Y, Hirata M, Akiyama H, Chung UI, Kawaguchi H
  • Journal Title: Proc Natl Acad Sci USA Volume: 110(5) Pages: 1875-80
• [Journal Article] ss-catenin regulates parathyroid hormone/parathyroid hormone-related protein receptor signals and chondrocyte hypertrophy through binding to the intracellular C-terminal region of the receptor (2013)
  • Author(s): Yano F, Saito T, Ogata N, Yamazawa T, Iino M, Chung UI, Kawaguchi H
  • Journal Title: Arthritis Rheum Volume: 65(2) Pages: 429-35
• [Journal Article] GSK-3α and GSK-3ss proteins are involved in early stages of chondrocyte differentiation with functional redundancy through RelA protein phosphorylation (2012)
  • Author(s): Itoh S, Saito T, Hirata M, Ushita M, Ikeda T, Woodgett JR, Algul H, Schmid RM, Chung UI, Kawaguchi H
  • Journal Title: J Biol Chem Volume: 287(35) Pages: 29227-36
• [Journal Article] C/EBPss and RUNX2 cooperate to degrade cartilage with MMP-13 as the target and HIF-2α as the inducer in chondrocytes (2012)
  • Author(s): Hirata M, Kugimiya F, Fukai A, Saito T, Yano F, Ikeda T, Mabuchi A, Sapkota BR, Akune T, Nishida N, Yoshimura N, Nakagawa T, Tokunaga K, Nakamura K, Chung UI, Kawaguchi H
  • Journal Title: Hum Mol Genet Volume: 21(5) Pages: 1111-23
• [Journal Article] Prevalence of symptomatic lumbar spinal stenosis and its association with physical performance in a population-based cohort in Japan: the Wakayama Spine Study (2012)
  • Author(s): Ishimoto Y, Yoshimura N, Muraki S, Yamada H, Nagata K, Hashizume H, Takiguchi N, Minamide A, Oka H, Kawaguchi H, Nakamura K, Akune T, Yoshida M
  • Journal Title: Osteoarthritis Cartilage Volume: 20(10) Pages: 1103-8
• [Journal Article] Lack of a chondroprotective effect of cyclooxygenase 2 inhibition in a surgically induced model of osteoarthritis in mice (2012)
  • Author(s): Fukai A, Kamekura S, Chikazu D, Nakagawa T, Hirata M, Saito T, Hosaka Y, Ikeda T, Nakamura K, Chung UI, Kawaguchi H
  • Journal Title: Arthritis Rheum Volume: 64(1) Pages: 198-203