Involvement of Toll-like receptors in the Actions of Opioids

Research Project

Project/Area Number	23659742
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Anesthesiology/Resuscitation studies
Research Institution	Kyoto University
Principal Investigator	KAKUYAMA Masahiro 京都大学, 医学研究科, 講師 (30273444)
Project Period (FY)	2011 – 2012
Project Status	Completed (Fiscal Year 2012)
Budget Amount *help	¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000) Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords	オピオイド / モルヒネ / 耐性 / マイクログリア / ノックアウトマウス / toll-like receptor
Research Abstract	To examine the hypothesis that the toll-like receptor 4 (TLR4) is involved in the morphine-induced activation of microglia, we analyzed morphine tolerance in TLR4 knockout mice and C3H/HeJ mice possessing mutated TLR4. It was shown that morphine tolerance was induced in both knockout and mutant mice similarly as wild-type mice, and suggested that TLR4 is not involved in morphine-induced activation of microglia. Our results suggest that morphine tolerance is mediated by microglia activated through the mechanism other than TLR4.

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(3 results)