| Project/Area Number |
23659845
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Emergency medicine
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| Research Institution | Shimane University |
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Principal Investigator |
ODA Teiji 島根大学, 医学部, 教授 (50448198)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUMOTO Ken-ihci 島根大学, 総合科学研究支援センター, 教授 (30202328)
SHIMIZU Kouji 島根大学, 医学部, 助教 (70548578)
橋口 尚幸 順天堂大学, 医学部, 教授 (70322188)
庄野 敦子 島根大学, 医学部, 助教 (90343285)
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| Co-Investigator(Renkei-kenkyūsha) |
MIHARA Toru 島根大学, 医学部, 助教 (30437537)
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| Project Period (FY) |
2011 – 2013
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | プロテオミクス / 低体温 / 脳低体温療法 / 免疫 / 副作用 / 血液 / 血清 / 赤血球 / 血小板 / 好中球 / 単球 |
|---|
| Research Abstract |
Therapeutic hypothermia after cardiac arrest is increasingly utilized, but its pleiotropic effects are as yet incompletely elucidated. In order to analyze these mechanisms, we comparatively investigated plasma proteomic changes induced by both induction of hypothermia and rewarming in nine patients undergoing therapeutic hypothermia. We utilized iTRAQ labeling tandem mass spectrometry to assess quantitative protein changes. In total, the analysis identified 188 proteins. Bioinformatic analyses showed significant up-regulation of serine protease inhibitor and defense/immunity protein at hypothermia. After rewarming, both apolipoprotein and complement component proteins were also significantly up-regulated. Out of nine patients, three patients have recovered without any neurological complications, but the other six patients have had serious neurological defects. These outcomes were associated with the levels of several proteins belonging to oxidative stress and innate immunity.
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