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2013 Fiscal Year Final Research Report

Proteomic analysis of the chnages in blood induced by therapeutic hypothermia

Research Project

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Project/Area Number 23659845
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Emergency medicine
Research InstitutionShimane University

Principal Investigator

ODA Teiji  島根大学, 医学部, 教授 (50448198)

Co-Investigator(Kenkyū-buntansha) MATSUMOTO Ken-ihci  島根大学, 総合科学研究支援センター, 教授 (30202328)
SHIMIZU Kouji  島根大学, 医学部, 助教 (70548578)
Co-Investigator(Renkei-kenkyūsha) MIHARA Toru  島根大学, 医学部, 助教 (30437537)
Project Period (FY) 2011 – 2013
Keywordsプロテオミクス / 低体温
Research Abstract

Therapeutic hypothermia after cardiac arrest is increasingly utilized, but its pleiotropic effects are as yet incompletely elucidated. In order to analyze these mechanisms, we comparatively investigated plasma proteomic changes induced by both induction of hypothermia and rewarming in nine patients undergoing therapeutic hypothermia. We utilized iTRAQ labeling tandem mass spectrometry to assess quantitative protein changes. In total, the analysis identified 188 proteins. Bioinformatic analyses showed significant up-regulation of serine protease inhibitor and defense/immunity protein at hypothermia. After rewarming, both apolipoprotein and complement component proteins were also significantly up-regulated. Out of nine patients, three patients have recovered without any neurological complications, but the other six patients have had serious neurological defects. These outcomes were associated with the levels of several proteins belonging to oxidative stress and innate immunity.

Research Products

(6 results)

All 2013 2012 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Remarks (1 results)

  • [Journal Article] Proteomic profiling for the identification of serum diagnostic biomarkers for abdominal and thoracic aortic aneurysms2013

    • Author(s)
      Kazumi Satoh, Tomoko Maniwa, Teiji Oda, Ken-ichi Matsumoto
    • Journal Title

      Proteome Science

      Volume: 11 Pages: 27

    • URL

      http://www.proteomesci.com/content/11/1/27

    • Peer Reviewed
  • [Journal Article] Proteomic comparison between abdominal and thoracic aortic aneurysms2013

    • Author(s)
      Ken-ichi Matsumoto, Kazumi Satoh, Tomoko Maniwa, Tetsuya Tanaka, Hideki Okunishi, Teiji Oda
    • Journal Title

      Int J Mol Med

      Volume: 33 Pages: 1035-1047

    • DOI

      10.3892/ijmm.2014.1627

    • Peer Reviewed
  • [Journal Article] Proteomic analysis of calcified abdominal and thoracic aortic aneurysms2012

    • Author(s)
      Ken-ichi Matsumoto, Tomoko Maniwa, Tetsuya Tanaka, Kazumi Satoh, Hideki Okunishi, Teiji Oda
    • Journal Title

      Int J Mol Med

      Volume: 30 Pages: 417-429

    • DOI

      10.3892/ijmm.2012.985

    • Peer Reviewed
  • [Journal Article] Hypothermia produces rat liver proteomic changes as in hibernating mammals but decreases endoplasmic reticulum chaperones2012

    • Author(s)
      Teiji Oda, Kouji Shimizu, Akane Yamaguchi, Kazumi Satoh, ken-ichi Matsumoto
    • Journal Title

      Cryobiology

      Volume: 65 Pages: 104-112

    • DOI

      10.1016/j.cryobiol.2012.05.004

    • Peer Reviewed
  • [Journal Article] Noticeable decreased expression of tenascin-X in calcific aortic valves2012

    • Author(s)
      Ken-ichi Matsumoto, Kazumi Satoh, Tomoko Maniwa, Asuka Araki, Riruke Maruyama, Teiji Oda
    • Journal Title

      Connective Tissue Research

      Volume: 53 Pages: 460-468

    • DOI

      10.3109/03008207.2012.702818

    • Peer Reviewed
  • [Remarks]

    • URL

      http://www.med.shimane-u.ac.jp/medicine/outline/2ge.html

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Published: 2015-06-25  

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