The involvement of Streptococcus anginosus infection and AID expression in oral cancers

• Project/Area Number: 23659861
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Morphological basic dentistry
• Research Institution: Iwate Medical University
• Principal Investigator: KODAMA YOSHITOYO 岩手医科大学, 歯学部, 非常勤講師 (70398486)
• Co-Investigator(Renkei-kenkyūsha): SASAKI Minoru 岩手医科大学, 歯学部, 准教授 (40187133) ; KIMURA Shigenobu 岩手医科大学, 歯学部, 教授 (10177917)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 感染発癌 / AID / 口腔細菌 / 口腔癌 / Streptococcus anginosus / 炎症 / S. anginosus / 歯肉上皮細胞 / 口腔レンサ球菌

Research Abstract

Streptococcus anginosus infection could be closely related with oral squamous cellcarcinoma. Recent studies suggested that the aberrant expression of AID in the epithelial cells could re-sult in the generation of nucleotide alterations in tumor-related genes and possible malignant transforma-tion of the AID-expressing cells. In this study, the involvement of S. anginosus infection and the AID ex-pression in oral cancers was assessed. S. anginosus DNA was frequently detected in the oral squamous cell carcinoma, and the relatively higher AID expressions were observed in the cancerous region. The stimula- tion with S. anginosus antigens could induce the NF-kB activation and AID expression in cultured cell li- nes. Taken together, S. anginosus infection could be closely related with the AID expression in the oral squamous cell carcinoma.

Research Products

• [Journal Article] 口腔癌とStreptococcus anginosus 感染 (2013)
  • Author(s): 佐々木実, 古玉芳豊, 木村重信
  • Journal Title: 岩医歯誌 Volume: 38 Pages: 45-52
  • NAID: 110009661242
• [Journal Article] 口腔癌とStreptococcus anginosus感染 (2013)
  • Author(s): 佐々木実, 古玉芳豊, 木村重信
  • Journal Title: 岩医歯誌 Volume: 38 Pages: 45-52
  • NAID: 110009661242
  • Peer Reviewed
• [Journal Article] Subcutaneous tissue reactions against nano-apatite collagen composites (2012)
  • Author(s): Hatakeyama, W., Taira, M., Y., Kihara, H., Sasaki, M., Kimura, S., Kondo, H
  • Journal Title: Nano Biomedicine Volume: 4 Pages: 118-124
  • NAID: 130004679846
  • Peer Reviewed
• [Journal Article] Quantatative real-time RT-PCR analyses of DNA-damage-recovery-related gene expressions of mouse macrophage-like cell line RAW264 when exposed to IC_<50> nickel ions (2012)
  • Author(s): Taira, M., Y., Kagiya, T., Sasaki, M., Kimura, S
  • Journal Title: Nano Biomedicine Volume: 3 Pages: 294-299
  • Peer Reviewed
• [Journal Article] Proteome analyses of human macrophages exposed to low cytotoxic IC90 copper (2+) ions (2011)
  • Author(s): Taira, M., Shimoyama, Y., Kagiya, T., Sasaki, M., Nezu, T., Harada, H. and Kimura, S
  • Journal Title: Dent. Mater. J Volume: 30 Pages: 293-299
  • NAID: 10029136627
  • Peer Reviewed
• [Journal Article] Quantitative real-time RT-PCR analyses of DNA-damage-recovery-related gene expressions of mouse macrophage-like cell line RAW264 when exposed to IC_<50> nickel ions (2011)
  • Author(s): Taira, M., Kagiya, T., Sasaki, M. and Kimura, S
  • Journal Title: Nano Biomed Volume: 3 Pages: 294-299
  • Peer Reviewed
• [Presentation] The involvement of Streptococcus anginosus infection and AID expression in oral cancers (2014)
  • Author(s): Sasaki, M., Kodama, Y., Ishikawa, T., Kimura, S
  • Organizer: 第87回日本細菌学会総会
  • Place of Presentation: 船堀,東京
  • Year and Date: 2014-03-28
• [Presentation] Infection of 'red complex species' to the plaque of children (2014)
  • Author(s): Shimoyama, Y., Kimura, M., Ohara-Nemoto, Y., Nemoto, T. K., Tanaka, M., Sasaki, M., Kimura, S
  • Organizer: 第87回日本細菌学会総会
  • Place of Presentation: 船堀,東京
  • Year and Date: 2014-03-26
• [Presentation] The involvement of Streptococcus anginosus infection and AID expression in oral cancers. (2014)
  • Author(s): Minoru Sasaki, Yoshitoyo Kodama, Taichi Ishikawa, Shigenobu Kimura
  • Organizer: 第87回日本細菌学会総会
  • Place of Presentation: 船堀，東京
• [Presentation] The involvement of Streptococcus anginosus infection and AID expression in oral cancers (2013)
  • Author(s): Iwasaki, K., Sasaki, M., Kodama, Y., Matsumoto, N., Kimura, S. and Sugiyama. Y
  • Organizer: 21st International Conference on Oral and Maxillofacial Surgery
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2013-10-21
• [Presentation] 口腔癌におけるStreptococcus anginosus 感染とAID 異所性発現 (2013)
  • Author(s): 佐々木実,古玉芳豊,下山 佑,木村重信
  • Organizer: 第55回歯科基礎医学会学術大会
  • Place of Presentation: 岡山市
  • Year and Date: 2013-09-22
• [Presentation] Distribution of Porphyromonas gingivalis fimA Genotypes in Children's plaques (2013)
  • Author(s): Shimoyama, Y., Ohara-Nemoto, Y., Kimura, S, Sasaki, M., Kodama, Y. Tanaka,M., Kimura, S
  • Organizer: 113^<th> General Meeting ASM
  • Place of Presentation: Denver, USA
  • Year and Date: 2013-05-19
• [Presentation] The aciduricity in Streptococcus anginosus (2013)
  • Author(s): Sasaki, M., Kodama, Y., Shimoyama, Y., Kimura, S
  • Organizer: 91th General Session & Exhibition of the International Association of Dental Research (IADR)
  • Place of Presentation: Siattle, USA
  • Year and Date: 2013-03-21
• [Presentation] Nested PCR を用いた小児口腔へのPorphyromonas gingivalis 線毛タイプ別感染実態の検討 (2013)
  • Author(s): 下山 佑,木村美澄,佐々木実,根本優子,古玉芳豊,上戸幸美,田中光郎,木村重信
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 幕張
  • Year and Date: 2013-03-20
• [Presentation] The involvement of Streptococcus anginosus infection and AID expression in oral cancers. (2013)
  • Author(s): Kensuke Iwasaki, Minoru Sasaki, Yoshitoyo Kodama, Naoko Matsumoto, Shigenobu Kimura and Yoshiki Sugiyama
  • Organizer: 21st International Conference on Oral and Maxillofacial Surgery
  • Place of Presentation: Barcelona, Spain
• [Presentation] 口腔癌におけるStreptococcus anginosus感染とAID異所性発現 (2013)
  • Author(s): 佐々木実，古玉芳豊，下山 佑，木村重信
  • Organizer: 第55回歯科基礎医学会学術大会
  • Place of Presentation: 岡山市
• [Presentation] Distribution of Porphyromonas gingivalis fimA Genotypes in Children’s plaques (2013)
  • Author(s): Y. Shimoyama, Y. Ohara-Nemoto, M. Kimura, M. Sasaki, Y. Kodama1, M. Tanaka, S. Kimura
  • Organizer: 113th General Meeting ASM
  • Place of Presentation: Denver, USA
• [Presentation] The aciduricity in Streptococcus anginosus. (2013)
  • Author(s): Sasaki, M.
  • Organizer: 91th General Session & Exhibition of the International Association of Dental Research
  • Place of Presentation: Seattle, USA
• [Presentation] Nested PCRを用いた小児口腔へのPorphyromonas gingivalis 線毛タイプ別感染実態の検討 (2013)
  • Author(s): 下山 佑
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 幕張，千葉
• [Presentation] Streptococcus anginosus の耐酸性に関わる酵素の役割 (2012)
  • Author(s): 佐々木実,古玉芳豊,下山 佑,木村重信
  • Organizer: 第54回歯科基礎医学会学術大会
  • Place of Presentation: 郡山
  • Year and Date: 2012-09-16
• [Presentation] A Novel Fibronectin Binding Protein of Streptococcus intermedius (2012)
  • Author(s): Sasaki, M., Kodama, Y., Shimoyama, Y., Kimura, S
  • Organizer: 90th General Session & Exhibition of the International Association of Dental Research (IADR)
  • Place of Presentation: Iguacu Falls, Brazil
  • Year and Date: 2012-06-21
• [Presentation] Streptococcus intermedius のフィブロネクチン結合タンパク質による上皮細胞付着機序 (2012)
  • Author(s): 古玉芳豊,佐々木 実,下山 佑,上戸幸美,木村重信
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎
  • Year and Date: 2012-03-27
• [Presentation] Streptococcus anginosusの耐酸性に関わる酵素の役割 (2012)
  • Author(s): 佐々木実
  • Organizer: 第54回歯科基礎医学会学術大会
  • Place of Presentation: 郡山，福島
• [Presentation] A Novel Fibronectin Binding Protein of Streptococcus intermedius. (2012)
  • Author(s): Sasaki, M.
  • Organizer: 90th General Session & Exhibition of the International Association of Dental Research
  • Place of Presentation: Iguas Falls, Brazil
• [Presentation] Streptococcus intermediusのフィブロネクチン結合タンパク質による上皮細胞付着機序 (2012)
  • Author(s): 古玉芳豊，佐々木 実，下山 佑，上戸幸美，木村重信
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎
• [Presentation] Streptococcus anginosus のフィブロネクチン結合タンパク質のマウスにおける病原性の解析 (2011)
  • Author(s): 佐々木 実,古玉芳豊,下山 佑,木村重信
  • Organizer: 第53回歯科基礎医学会学術大会
  • Place of Presentation: 岐阜
  • Year and Date: 2011-10-01
• [Presentation] The pathogenic involvement of the fibronectin binding protein, Fbp62, in Streptococcus anginosus infection (2011)
  • Author(s): Sasaki, M., Kodama, Y., Shimoyama, Y., Agato, S. and Kimura, S
  • Organizer: International Union of Microbiological Societies 2011 Congress (IUMS 2011)
  • Place of Presentation: Sapporo, Japan
  • Year and Date: 2011-09-07
• [Presentation] Fbp62, fibronectin binding protein of Streptococcus anginosus involves the virulence in mouse infection (2011)
  • Author(s): Sasaki, M., Kodama, Y., Shimoyama, Y., Ishikawa, T. and Kimura, S
  • Organizer: 10th World Congress on Inflammation 2011
  • Place of Presentation: Paris, France
  • Year and Date: 2011-06-26
• [Presentation] Auto-catalytic propeptide processing facilitates the final hetero-catalytic maturation of glutamyl endopeptidase GluV8 (2011)
  • Author(s): Ohara-Nemoto, Y., Rouf, S.M.A., Shimoyama, Y., Kimura, S., Ono, T. and Nemoto, T.K
  • Organizer: 111th General Meeting of the American Society for Microbiology
  • Place of Presentation: New Orleans, USA
  • Year and Date: 2011-05-24
• [Presentation] Fibronectin binding activity of Streptococcus anginosus promotes the adherence to mucosal epithelial cells (2011)
  • Author(s): Sasaki, M., Kodama, Y., Shimoyama, Y., Ishikawa, T. and Kimura, S
  • Organizer: 4th International Symposium for Interface Oral Health Science in Sendai
  • Place of Presentation: Sendai, Japan
  • Year and Date: 2011-03-08
• [Presentation] Rapid identification of Abiotrophia/Granulicatella species using by 16S rRNA based PCR and RFLP (2011)
  • Author(s): Shimoyama, Y., Sasaki, M., Ohara-Nemoto, Y., Nemoto, T. K., Ishikawa, T. and Kimura, S
  • Organizer: 4th International Symposium for Interface Oral Health Science in Sendai
  • Place of Presentation: Sendai, Japan
  • Year and Date: 2011-03-08
• [Presentation] Maternal transmission of mutans and other oral streptococcal species (2011)
  • Author(s): Otake-Asakawa, A., Harada-Oikawa, R., Ohara-Nemoto, Y., Tanaka, M. and Kimura, S
  • Organizer: 4th International Symposium for Interface Oral Health Science in Sendai
  • Place of Presentation: Sendai, Japan
  • Year and Date: 2011-03-08
• [Presentation] In vitro adherence of Candida albicans to acrylic resin with different surface status (2011)
  • Author(s): Nomura, T., Suzuki, T., Furuya, J., Shimoyama, Y., Sasaki, M. and Kimura, S
  • Organizer: 4th International Symposium for Interface Oral Health Science in Sendai
  • Place of Presentation: Sendai, Japan
  • Year and Date: 2011-03-08
• [Presentation] Macrophage reaction against sub-micron titanium particles (2011)
  • Author(s): Taira, M., Sasaki, M. and Kimura, S
  • Organizer: 4th International Symposium for Interface Oral Health Science in Sendai
  • Place of Presentation: Sendai, Japan
  • Year and Date: 2011-03-08
• [Presentation] The pathogenic involvement of the fibronectin binding protein, Fbp62, in Streptococcus anginosus infection. (2011)
  • Author(s): Sasaki, M., Kodama, Y., Shimoyama, Y., Agato, S. and Kimura, S.
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: Sapporo
• [Presentation] Fbp62, fibronectin binding protein of Streptococcus anginosus involves the virulence in mouse infection. (2011)
  • Author(s): Sasaki, M., Kodama, Y., Shimoyama, Y., Ishikawa, T. and Kimura, S.
  • Organizer: 10th World Congress on Inflammation 2011
  • Place of Presentation: Paris
• [Presentation] Streptococcus anginosusのフィブロネクチン結合タンパク質のマウスにおける病原性の解析 (2011)
  • Author(s): 佐々木 実，古玉芳豊，下山 佑，木村重信
  • Organizer: 第53回歯科基礎医学会学術大会
  • Place of Presentation: 岐阜
• [Book] Pathogenic factors of Porphyromonas gingivalis and the host difence mechanisms. In Periodontitis (Pathogenesis and Treatment of Periodontitis) (2012)
  • Author(s): Kimura, S., Ohara-Nemoto, Y., Shimoyama, Y., Ishikawa, T., and Sasaki, M
  • Publisher: InTech
• [Book] Fibronectin binding activity of Streptococcus anginosus promotes the adherence to mucosal epithelial cells. In Interface Oral Health Science 2011 (Watanabe, M. et al., eds.) (2012)
  • Author(s): Sasaki, M., Kodama, Y., Shimoyama, Y., Ishikawa, T. and Kimura, S
  • Publisher: Springer Japan, Tokyo
• [Book] Rapid identification of Abiotrophia/Granulicatella species using by 16S rRNA based PCR and RFLP. In Interface Oral Health Science 2011 (Watanabe, M. et al., eds.) (2012)
  • Author(s): Shimoyama, Y., Sasaki, M., Ohara-Nemoto, Y., Nemoto, T. K., Ishikawa, T. and Kimura, S
  • Publisher: Springer Japan, Tokyo
• [Book] Maternal transmission of mutans and other oral streptococcal species. In Interface Oral Health Science 2011 (Watanabe, M. et al., eds.) (2012)
  • Author(s): Otake-Asakawa, A., Harada-Oikawa, R., Ohara-Nemoto, Y., Tanaka, M. and Kimura, S
  • Publisher: Springer Japan, Tokyo
• [Book] In vitro adherence of Candida albicans to acrylic resin with different surface status. In Interface Oral Health Science 2011 (Watanabe, M. et al., eds.) (2012)
  • Author(s): Nomura, T., Suzuki, T., Furuya, J., Shimoyama, Y., Sasaki, M. and Kimura, S
  • Publisher: Springer Japan, Tokyo
• [Book] Macrophage reaction against sub-micron titanium particles. In Interface Oral Health Science 2011 (Watanabe, M. et al., eds.) (2012)
  • Author(s): Taira, M., Sasaki, M. and Kimura, S
  • Publisher: Springer Japan, Tokyo
• [Book] Interface Oral Health Science 2011 (2012)
  • Author(s): Sasaki, M.
  • Total Pages: 422
  • Publisher: Springer
• [Book] Interface Oral Health Science 2011 (2012)
  • Author(s): Sasaki, M., Kodama, Y., Shimoyama, Y., Ishikawa, T. and Kimura, S.
  • Publisher: Springer