Visualization of the process from generation to exocytosis of secretory granules by expression of fluorescent dye-binding proteins
Project/Area Number |
23659875
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Functional basic dentistry
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Research Institution | Nihon University |
Principal Investigator |
|
Project Period (FY) |
2011 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
|
Keywords | 口腔生理学 / ライブイメージング / 唾液腺 / 分泌顆粒 / 調節性分泌 / 輸送シグナル / 開口放出 / シグナル配列 / HaloTag |
Research Abstract |
To visualize the process from generation to exocytosis of secretory granules, we established an expression system of the fluorescent dye-binding protein HaloTag in salivary gland cells. We found that a HaloTag protein having only a signal peptide sequence was sorted into secretory granules of salivary glands. We succeeded in live imaging experiments of stimulus-dependent exocytosis by labeling secretory granules with fluorescent HaloTag ligands.
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Report
(3 results)
Research Products
(12 results)
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[Journal Article] <b>Effects of [6]-Gingerol on Dedifferentiation of Salivary Acinar Cells </b>2013
Author(s)
Yokoyama M, Haruki M, Matsuki-Fukushima M, Fuse-Nagai M, Fukumoto M, Narita K, Okabayashi K, Sugiya H, Katsumata-Kato O, Fujita-Yoshigaki J.
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Journal Title
International Journal of Oral-Medical Sciences
Volume: 11
Issue: 4
Pages: 315-319
DOI
NAID
ISSN
1347-9733, 2185-4254
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