| Project/Area Number |
23659875
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Functional basic dentistry
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| Research Institution | Nihon University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 口腔生理学 / ライブイメージング / 唾液腺 / 分泌顆粒 / 調節性分泌 / 輸送シグナル / 開口放出 / シグナル配列 / HaloTag |
|---|
| Research Abstract |
To visualize the process from generation to exocytosis of secretory granules, we established an expression system of the fluorescent dye-binding protein HaloTag in salivary gland cells. We found that a HaloTag protein having only a signal peptide sequence was sorted into secretory granules of salivary glands. We succeeded in live imaging experiments of stimulus-dependent exocytosis by labeling secretory granules with fluorescent HaloTag ligands.
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