Analysis of circularly polarized luminescence from DNA quadruplex

• Project/Area Number: 23685031
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Functional materials chemistry
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: YUASA Junpei 奈良先端科学技術大学院大学, 物質創成科学研究科, 助教 (00508054)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥28,990,000 (Direct Cost: ¥22,300,000、Indirect Cost: ¥6,690,000); Fiscal Year 2013: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000); Fiscal Year 2012: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000); Fiscal Year 2011: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
• Keywords: 円偏光発光 / DNA4重鎖 / プローブ / タンパク / 構造変化 / 変性 / 時間分解 / CPL / キラリティー / 希土類錯体 / 超分子 / 蛍光プローブ / 光化学 / カルバゾール / 発光体 / DNA２重鎖

Research Abstract

We report herein differentiation of thermal stability of calf thymus (ct-) DNA and enzymatic activity of deoxyribonuclease (DNase I) by a change in the binding state between dicationic carbazole ligands and ct-DNA.

Research Products

• [Journal Article] Ratiometric luminescence thermometry based on crystal-field alternation at the extremely narrow ^5D_0 - ^7F_2 transition band of europium(III) (2014)
  • Author(s): Junpei Yuasa, Ryusuke Mukai, Tsuyoshi Kawai
  • Journal Title: Chem. Commun Volume: 50 Issue: 59 Pages: 12-54
  • DOI: 10.1039/c4cc00704b
  • Peer Reviewed
• [Journal Article] Phosphorescent properties of metal-free diphosphine ligands and effects of copper binding (2013)
  • Author(s): Junpei Yuasa, Misato Dan, Tsuyoshi Kawai
  • Journal Title: Dalton Trans Volume: 42 Pages: 16096-16101
• [Journal Article] Fingerprint Signatures of Lanthanide Circularly Polarized Luminescence from Proteins Covalently Labeled by aβ-Diketonate Europium(III) Chelate (2013)
  • Author(s): Junpei Yuasa, Tomoko Ohno, Hiroyuki Tsumatori, Rumi Shiba, Hironari Kamikubo, Mikio Kataoka, Yasuchika Hasegawa, Tsuyoshi Kawai
  • Journal Title: Chem. Commun Volume: 49
• [Journal Article] Two Distinct Thermal Stabilities of DNA and Enzymatic Activities of DNase I in a Multistep Assembly with Carbazole Ligands. Different Binding Characteristics for Duplex and Quadruplex DNA (2013)
  • Author(s): Norie Inukai, Tusyoshi Kawai, Junpei Yuasa
  • Journal Title: Chem. –Eur. J Volume: 19
• [Journal Article] Phosphorescent properties of metal-free diphosphine ligands and effects of copper binding (2013)
  • Author(s): Junpei Yuasa, Misato Dan and Tsuyoshi Kawai
  • Journal Title: Dalton Transactions Volume: 42 Issue: 45 Pages: 16096-16101
  • DOI: 10.1039/c3dt51390d
  • Peer Reviewed
• [Journal Article] Fingerprint signatures of lanthanide circularly polarized luminescence from proteins covalently labeled by a β -diketonate europium(III) chelate (2013)
  • Author(s): Junpei Yuasa
  • Journal Title: Chem. Commn. Volume: 49 Issue: 41 Pages: 4604-4606
  • DOI: 10.1039/c3cc40331a
  • Peer Reviewed
• [Journal Article] Two Distinct Thermal Stabilities of DNA and Enzymatic Activities of DNase I in a Multistep Assembly with Carbazole Ligands : Different Binding Characteristics for Duplex and Quadruplex DNA (2013)
  • Author(s): Norie Inukai, Tsuyoshi Kawai, Junpei Yuasa
  • Journal Title: Chemistry - A European Journal Volume: 19(印刷中) Issue: 19 Pages: 5938-5947
  • DOI: 10.1002/chem.201203461
  • Peer Reviewed
• [Journal Article] Nocovalent Ligand-to-Ligand Interactions Alter Sense of Optical Chirality in Luminescent Tris(β-diketonate) Lanthanide(III) Complexes Containing a Chiral Bis(oxazolinyl) Pyridine Ligand (2011)
  • Author(s): Junpei Yuasa , Tomoko Ohno, Kohei Miyata, Hiroyuki Tsumatori, Yasuchika Hasegawa, Tsuyoshi Kawai
  • Journal Title: J. Am. Chem. Soc Volume: 133
• [Journal Article] Highly Photoluminescent Nanocrystals Based on a Gold(I) Complex and their Electrophoretic Patterning (2011)
  • Author(s): H Masashi Saitoh, Alan L. Balch, Junpei Yuasa, Kazuya Tada, Mitsuyoshi Onoda, Takuya Nakashima, Tsuyoshi Kawai
  • Journal Title: Langmuir Volume: 27
• [Journal Article] Mechanistic Understanding of Multistep Assembly of DNA with Carbazole Ligand by Simple Adjustment of Host-Guest Concentrations (2011)
  • Author(s): Norie Inukai, Tusyoshi Kawai, Junpei Yuasa
  • Journal Title: Chem. Commun Volume: 47
• [Journal Article] Highly Photoluminescent Nanocrystals based on a Gold(I) Complex and their Electrophoretic Patterning (2011)
  • Author(s): M.Saitoh, A.Balch, J.Yuasa, K.Tada, M.Onoda, T.Nakashima, T.Kawai
  • Journal Title: Langmuir Volume: 27 Pages: 10947-10952
  • Peer Reviewed
• [Journal Article] Nocovalent Ligand-to-Ligand Interactions Alter Sense of Optical Chirality in Luminescent Tris(β-diketonate) Lanthanide(III) Complexes Containing a Chiral Bis(oxazolinyl) Pyridine Ligand (2011)
  • Author(s): Junpei Yuasa, Tomoko Ohno, Kohei Miyata, Hiroyuki Tsumatori, Yasuchika Hasegawa, Tsuyoshi Kawai
  • Journal Title: J.Am.Chem.Soc. Volume: 133 Pages: 9892-9902
  • Peer Reviewed
• [Presentation] 段階的複合体形成を利用した新規蛍光プローブの開発 (2013)
  • Author(s): 湯浅順平
  • Organizer: 2013年光化学討論会
  • Place of Presentation: 愛媛大学
  • Year and Date: 2013-09-13
• [Presentation] 架橋配位子と亜鉛イオンとの段階的複合体形成に基づく円二色性スペクトルの反転現象 (2013)
  • Author(s): 湯浅順平、照沼卓也、河合壯
  • Organizer: 第25回配位化合物の光化学討論会
  • Place of Presentation: 唐津ロイヤルホテル
  • Year and Date: 2013-08-06
• [Presentation] 架橋配位子と亜鉛イオンとの段階的 複合体形成に基づく円二色性スペクトルの反転現象 (2013)
  • Author(s): 湯浅 順平、照沼 卓也、河合 壯
  • Organizer: 第25回配位化合物の光化学討論会
  • Place of Presentation: 唐津ロイヤルホテル
• [Presentation] Analysis of Circular-Polarized Luminescence of Proteins Labeled with Fluorescent Probes (2012)
  • Author(s): Junpei Yuasa
  • Organizer: 揺らぎと生体機能第6回公開シンポジウム
  • Place of Presentation: 京都テルサ
  • Year and Date: 2012-12-05
• [Presentation] CIRCULAR-POLARIZED LUMINESCENCE OF PROTEINS LABELED WITH LANTHANIDE COMPLEX (2012)
  • Author(s): Junpei Yuasa, Tomoko Ohno, Hiroyuki Tsumatori, Yasuchika Hasegawa, Tsuyoshi Kawai
  • Organizer: 揺らぎが決める生命機能第5回公開シンポジウム
  • Place of Presentation: 公開市民講演会、奈良市
  • Year and Date: 2012-01-07
• [Presentation] 水溶性ユーロピウム錯体の合成と温度依存スペクトル形状変化を利用した精密温度センシング (2011)
  • Author(s): 湯浅順平・向隆介・長谷川靖哉・河合壯
  • Organizer: 2011年光化学討論会
  • Place of Presentation: 宮崎市河畔コンベンションエリア
  • Year and Date: 2011-09-08
• [Presentation] Spectroscopic Analysis of Circular-Polarized Luminescence of Proteins Labeled with Fluorescent Probes (2011)
  • Author(s): Junpei Yuasa
  • Organizer: 5th Asia and Oceania Conference for Photobiology "AOCP2011"
  • Place of Presentation: Nara Prefectural New Public Hall, Nara, Japan
  • Year and Date: 2011-07-30
• [Presentation] 円偏光発光性錯体の合成と発光特性 (2011)
  • Author(s): 湯浅順平
  • Organizer: 配位プログラミング第1回若手フォーラム
  • Place of Presentation: 東京大学
  • Year and Date: 2011-05-20
• [Presentation] 円偏光発光性錯体の合成と発光特性 (2011)
  • Author(s): 湯浅順平
  • Organizer: 配位プログラミング第1回若手フォーラム
  • Place of Presentation: 東京大学・理学部
  • Year and Date: 2011-05-20
• [Presentation] 亜鉛イオンとの相互作用によって誘起されるテトラジン誘導体の蛍光とそれを利用した金属イオンセンシング (2011)
  • Author(s): 湯浅順平・三井亮人・河合壯
  • Organizer: 第23回配位化合物の光化学討論会
  • Place of Presentation: 信州大学繊維学部
• [Presentation] Analysis of Circular-Polarized Luminescence of Proteins Labeled with Fluorescent Probes
  • Author(s): Junpei Yuasa
  • Organizer: 第6回公開国際シンポジウム 新学術領域 揺らぎと生体機能
  • Place of Presentation: 京都テルサ
  • Invited
• [Patent(Industrial Property Rights)] 円偏光発光性希土類錯体 (2011)
  • Inventor(s): 湯浅順平、上野紘史、 河合壯、長谷川靖哉
  • Industrial Property Rights Holder: 奈良先端科学技術大学院大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2011-269916
  • Filing Date: 2011-12-09
  • Overseas
• [Patent(Industrial Property Rights)] 円偏光発光性希土類錯体 (2011)
  • Inventor(s): 湯浅順平、上野紘史、河合壯、長谷川靖哉
  • Industrial Property Rights Holder: 奈良先端科学技術大学院大学
  • Industrial Property Number: 2011-269916
  • Filing Date: 2011-12-09