Investigation of molecular mechanism for sexual isolation using cichlid and medaka
| Project/Area Number |
23687011
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biodiversity/Systematics
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| Research Institution | Japan Women's University |
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Principal Investigator |
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥14,170,000 (Direct Cost: ¥10,900,000、Indirect Cost: ¥3,270,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2012: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2011: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
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| Keywords | シクリッド / メダカ / 交尾前生殖隔離 / 同所的種分化 / 色素細胞 / 遺伝子操作 / 性選択 / 種分化 / ドーパミン / 体色 |
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| Research Abstract |
Chromosomal mapping of the locus responsible for dimorphic coloration (Gold vs Normal) in Midas cichlid was performed. The candidate region contained about 7 genes, and the dopamine D2-like receptor gene could be the most promising candidate as the responsible gene. Indeed, we found an amino-acid substitution between the Gold and Normal alleles, which would affect function of the receptor. Comparison of this amino acid among vertebrates indicated that the Normal allele is derived from the Gold allele. We established genetically modified medaka strains of this gene; namely, missense mutants, frame-shift mutants, and transgenic strains that constitutively express the Gold or Normal allele. In terms of the missense mutants, we found that mutants were less viable than wild-type siblings, but causal relationship between body color and drd2-like remained unknown. Other strains established in this study will be helpful for further investigation of the function of this receptor.
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Report
(4 results)
Research Products
(6 results)
