Development of microfluidic mixer for time-resolved spectroscopy applicable to low-yield biomolecules
| Project/Area Number |
23687022
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (A)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Biophysics
|
|---|
| Research Institution | Institute of Physical and Chemical Research (2013)
Institute for Molecular Science (2011-2012) |
|---|
Principal Investigator |
KIMURA Tetsunari 独立行政法人理化学研究所, 放射光科学総合研究センター, 研究員 (70506906)
|
|---|
| Project Period (FY) |
2011-04-01 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥28,470,000 (Direct Cost: ¥21,900,000、Indirect Cost: ¥6,570,000)
Fiscal Year 2013: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥20,150,000 (Direct Cost: ¥15,500,000、Indirect Cost: ¥4,650,000)
|
|---|
| Keywords | マイクロ流路 / フォールディング / 膜タンパク質 / フローセル / ストップト・フロー / マイクロ流路セル / 蛍光顕微観察 / ケージド化合物 / ヘムタンパク質 / マイクロ流体デバイス / 二重蛍光標識 / マイクロミキサー / 生体分子 / 蛍光測定 / 化学反応 |
|---|
| Research Abstract |
To understand the molecular mechanism of biomolecular reactions, the dynamics of the molecules along the reaction have to be revealed by the real-time measurements. However, the yield of protein is usually very low, ug-mg, and the time-resolved measurements is hard to apply for many biomolecular reactions because of the relatively large consumption. In this research, the time-resolved spectroscopies applicable to the low-yield protein samples were developed and applied to observe the folding, membrane transport and enzymatic reactions.
|
Report
(4 results)
Research Products
(27 results)
-
-
[Journal Article] Formation of Host-Guest Complexes on Gold Surface Investigated by Surface-Enhanced IR Absorption Spectroscopy2014
Author(s)
Inokuchi, Y., Mizuuchi, T., Ebata, T., Ikeda, T., Haino, T., Kimura, T., Guo, H., Furutani, Y
-
Journal Title
Chem. Phys. Lett
Volume: 592
Pages: 90-95
DOI
NAID
Related Report
Peer Reviewed / Open Access
-
-
-
[Journal Article] Dynamics of Dangling Bonds of Water Molecules in pharaonis Halorhodopsin during Chloride Ion Transportation2012
Author(s)
Furutani, Y., Fujiwara, K., Kimura, T., Kikukawa, T., Demura, M., Kandori, H
-
Journal Title
J. Phys. Chem. Lett
Volume: 3
Issue: 20
Pages: 2964-2969
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
