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Development of microfluidic mixer for time-resolved spectroscopy applicable to low-yield biomolecules

Research Project

Project/Area Number 23687022
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Biophysics
Research InstitutionInstitute of Physical and Chemical Research (2013)
Institute for Molecular Science (2011-2012)

Principal Investigator

KIMURA Tetsunari  独立行政法人理化学研究所, 放射光科学総合研究センター, 研究員 (70506906)

Project Period (FY) 2011-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥28,470,000 (Direct Cost: ¥21,900,000、Indirect Cost: ¥6,570,000)
Fiscal Year 2013: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥20,150,000 (Direct Cost: ¥15,500,000、Indirect Cost: ¥4,650,000)
Keywordsマイクロ流路 / フォールディング / 膜タンパク質 / フローセル / ストップト・フロー / マイクロ流路セル / 蛍光顕微観察 / ケージド化合物 / ヘムタンパク質 / マイクロ流体デバイス / 二重蛍光標識 / マイクロミキサー / 生体分子 / 蛍光測定 / 化学反応
Research Abstract

To understand the molecular mechanism of biomolecular reactions, the dynamics of the molecules along the reaction have to be revealed by the real-time measurements. However, the yield of protein is usually very low, ug-mg, and the time-resolved measurements is hard to apply for many biomolecular reactions because of the relatively large consumption. In this research, the time-resolved spectroscopies applicable to the low-yield protein samples were developed and applied to observe the folding, membrane transport and enzymatic reactions.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (27 results)

All 2014 2013 2012 2011

All Journal Article (7 results) (of which Peer Reviewed: 7 results,  Open Access: 1 results) Presentation (18 results) Book (2 results)

  • [Journal Article] 急速緩衝液交換法による時間分解全反射赤外分光法の開発2014

    • Author(s)
      古谷祐詞、木村哲就、岡本基士
    • Journal Title

      生物物理

      Volume: 印刷中

    • NAID

      130004693957

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Formation of Host-Guest Complexes on Gold Surface Investigated by Surface-Enhanced IR Absorption Spectroscopy2014

    • Author(s)
      Inokuchi, Y., Mizuuchi, T., Ebata, T., Ikeda, T., Haino, T., Kimura, T., Guo, H., Furutani, Y
    • Journal Title

      Chem. Phys. Lett

      Volume: 592 Pages: 90-95

    • DOI

      10.1016/j.cplett.2013.12.026

    • NAID

      120005615680

    • Related Report
      2013 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of a rapid Buffer-exchange system for time-resolved ATR-FTIR spectroscopy with the step-scan mode2013

    • Author(s)
      Furutani, Y., Kimura, T., Okamoto, K
    • Journal Title

      BIOPHYSICS

      Volume: 9 Issue: 0 Pages: 123-129

    • DOI

      10.2142/biophysics.9.123

    • NAID

      130003366039

    • ISSN
      1349-2942
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Distortion of the amide-I and -II bands of an α-helical membrane protein, pharaonis halorhodopsin, depends on thickness of gold film utilized for surface-enhanced infrared absorption spectroscopy2013

    • Author(s)
      Guo, H.
    • Journal Title

      Chem. Phys.

      Volume: in press

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dynamics of Dangling Bonds of Water Molecules in pharaonis Halorhodopsin during Chloride Ion Transportation2012

    • Author(s)
      Furutani, Y., Fujiwara, K., Kimura, T., Kikukawa, T., Demura, M., Kandori, H
    • Journal Title

      J. Phys. Chem. Lett

      Volume: 3 Issue: 20 Pages: 2964-2969

    • DOI

      10.1021/jz301287n

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Direct visualization reveals dynamics of a transient intermediate during protein assembly2011

    • Author(s)
      Zhang X, Lam VQ, Mou Y, Kimura T, Chung J, Chandrasekar S, Winkler JR, Mayo SL, Shan SO
    • Journal Title

      Proc. Natl. Acad. Sci

      Volume: 108 Issue: 16 Pages: 6450-6455

    • DOI

      10.1073/pnas.1019051108

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Direct visualization reveals dynamics of a transient intermediate during protein assembly2011

    • Author(s)
      Zhang X, Lam VQ, Mou Y, Kimura T, Chung J, Chandrasekar S, Winkler JR, Mayo SL, Shan SO
    • Journal Title

      Proc.Natl.Acad.Sci.U.S.A.

      Volume: 108 Pages: 6450-6455

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] フロー法を用いたタンパク質分子反応機構の実時間解析2014

    • Author(s)
      木村哲就
    • Organizer
      分子システム研究第3回春期研究会
    • Place of Presentation
      彦根市
    • Related Report
      2013 Annual Research Report
  • [Presentation] フロー・フラッシュ時間分解分光法による一酸化窒素還元酵素のNO還元反応機構の解析2014

    • Author(s)
      木村哲就、當舎武彦、城宜嗣、久保稔
    • Organizer
      第14回日本蛋白質科学会
    • Place of Presentation
      横浜市
    • Related Report
      2013 Annual Research Report
  • [Presentation] 酵素反応の時間分解分光解析を実現するマイクロ流路フローフラッシュ法の開発2013

    • Author(s)
      木村哲就,久保稔,當舎武彦,城宜嗣
    • Organizer
      日本生物物理学会第51回年会
    • Place of Presentation
      国立京都国際会館(京都市)
    • Related Report
      2013 Final Research Report
  • [Presentation] マイクロ流路を利用した時間分解分光計測系の構築2013

    • Author(s)
      木村哲就
    • Organizer
      分子システム研究第2回春期研究会
    • Place of Presentation
      静岡県御殿場市
    • Related Report
      2013 Annual Research Report
  • [Presentation] Development of micro-channel flow-flash method for time-resolved spectroscopic study of enzymatic reactions.2013

    • Author(s)
      木村哲就、當舎武彦、城宜嗣、久保稔
    • Organizer
      日本生物物理学会第51回年会
    • Place of Presentation
      京都市
    • Related Report
      2013 Annual Research Report
  • [Presentation] 生体分子反応の実時間観察を可能にする溶液混合装置の開発と応用例2012

    • Author(s)
      木村哲就
    • Organizer
      分子科学研究所技術課セミナー『フォトリソグラフィー技術の基礎と応用』
    • Place of Presentation
      岡崎カンファレンスセンター(岡崎市)
    • Year and Date
      2012-03-26
    • Related Report
      2013 Final Research Report
  • [Presentation] 体分子反応の実時間観察を可能にする溶液混合装置の開発と応用例2012

    • Author(s)
      木村哲就
    • Organizer
      分子科学研究所技術課セミナー「フォトリソグラフィー技術の基礎と応用」
    • Place of Presentation
      岡崎カンファレンスセンター(愛知県)
    • Year and Date
      2012-03-26
    • Related Report
      2011 Annual Research Report
  • [Presentation] 全反射赤外分光法によるMg^<2+>輸送体MgtEのイオン選択機構の解析2012

    • Author(s)
      木村哲就, 道喜慎太郎, 石谷隆一郎, 濡木理, 古谷祐詞
    • Organizer
      2011年度日本生物物理学会中部支部講演会
    • Place of Presentation
      名古屋大学野依記念学術交流館(愛知県)
    • Year and Date
      2012-03-19
    • Related Report
      2011 Annual Research Report
  • [Presentation] 低収量生体分子の時間分解分光計測を目指した新規溶液交換・混合装置の開発2012

    • Author(s)
      木村哲就、古谷祐詞
    • Organizer
      日本生物物理学会第50回年会
    • Place of Presentation
      名古屋大学(名古屋市)
    • Related Report
      2013 Final Research Report
  • [Presentation] 全反射赤外分光法によるMg^2+輸送体MgtEのイオン選択およびゲーティング機構の解析2012

    • Author(s)
      木村哲就、道喜慎太郎、石谷隆一郎、濡木理、古谷祐詞
    • Organizer
      第39回生体分子科学討論会
    • Place of Presentation
      東北大学片平さくらホール(仙台市)
    • Related Report
      2013 Final Research Report
  • [Presentation] Stepwise conformational changes upon anion pumping during the pharaonis halorhodopsin photocycle revealed by H/D unexchangeable amide-A vibrations2012

    • Author(s)
      Kimura, T., Kandori, H., and Furutani, Y
    • Organizer
      5th International Symposium on Nanomedicine (ISNM2011)
    • Place of Presentation
      名古屋大学ESホール(愛知県)
    • Related Report
      2013 Final Research Report 2011 Annual Research Report
  • [Presentation] Ion-selective mechanism of Mg^<2+> transporter MgtE studied by attenuated total refrection FTIR spectroscopy2012

    • Author(s)
      Kimura, T., Douki, S., Ishitani, R., Nureki, O., Furutani, Y
    • Organizer
      4^<th> Japan-Korea Seminar on Biomolecular Sciences
    • Place of Presentation
      東大寺文化センター(奈良県)
    • Related Report
      2013 Final Research Report
  • [Presentation] 全反射赤外分光法による Mg2+輸送体MgtEのイオン選択 およびゲーティング機構の解析2012

    • Author(s)
      木村哲就
    • Organizer
      第39回生体分子科学討論会
    • Place of Presentation
      仙台市(東北大学)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Development of novel rapid buffer-exchange system and microfluidic mixer for time-resolved spectroscopic study of low-yield biomolecules.2012

    • Author(s)
      Tetsunari Kimura
    • Organizer
      第50回日本生物物理学会年会
    • Place of Presentation
      名古屋市(名古屋大学)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Ion-selective mechanism of Mg^<2+> transporter MgtE studied by attenuated total refrection FTIR spectroscopy2012

    • Author(s)
      Tetsunari Kimura, Shintaro Douki, Ryuichiro Ishitani, Osamu Nureki, Yuji Furutani
    • Organizer
      4^<th> Japan-Korea Seminar on Biomolecular Sciences-Experiments and Simulations
    • Place of Presentation
      東大寺文化センター(奈良県)
    • Related Report
      2011 Annual Research Report
  • [Presentation] 全反射赤外分光法によるMg^<2+>輸送体MgtEのイオン選択機構の解析2011

    • Author(s)
      木村哲就、道喜慎太郎、石谷隆一郎、濡木理、古谷祐詞
    • Organizer
      第11回日本蛋白質科学会年会
    • Place of Presentation
      ホテル阪急エキスポパーク(大阪府)
    • Related Report
      2011 Annual Research Report
  • [Presentation] 全反射赤外分光法によるMg^<2+>輸送体MgtEのイオン選択機構の解析2011

    • Author(s)
      木村哲就、道喜慎太郎、石谷隆一郎、濡木理、古谷祐詞
    • Organizer
      第49回日本生物物理学会年会
    • Place of Presentation
      兵庫県立大学姫路書写キャンパス(兵庫県)
    • Related Report
      2011 Annual Research Report
  • [Presentation] 全反射赤外分光法によるMg^<2+>輸送体MgtEのイオン選択機構の解析2011

    • Author(s)
      木村哲就、道喜慎太郎、石谷隆一郎、濡木理、古谷祐詞
    • Organizer
      生体エネルギー研究会第37回討論会
    • Place of Presentation
      京都産業大学神山ホール(京都府)
    • Related Report
      2011 Annual Research Report
  • [Book] Watchi ng Dynamicsl Events in Protein Folding in th e Time domain from Submilliseconds to Seco nds : Continuous-Flow Rapid-Mixing Infrared Spectroscopy. in Protein Folding and Misfold ing : Shining Light by Infrared Spectroscopy.2011

    • Author(s)
      Takahashi, S. and Kimura, T( eds. Fabian, H., Naumann, D.)
    • Related Report
      2013 Final Research Report
  • [Book] Protein Folding and Misfolding : Shining Light by Infrared Spectroscopy. "Watching Dynamicsl Events in Protein Folding in the Time domain from Submilliseconds to Seconds : Continuous-Flow Rapid-Mixing Infrared Spectroscopy."2011

    • Author(s)
      S.Takahashi, T.KIMURA
    • Total Pages
      26
    • Publisher
      Springer-Verlag
    • Related Report
      2011 Annual Research Report

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Published: 2011-04-06   Modified: 2019-07-29  

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