Identification of genes involved in flower longevity
Project/Area Number |
23688004
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Research Category |
Grant-in-Aid for Young Scientists (A)
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Allocation Type | Single-year Grants |
Research Field |
Horticulture/Landscape architecture
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Research Institution | Tokyo University of Agriculture and Technology |
Principal Investigator |
YAMADA Tetsuya 東京農工大学, (連合)農学研究科(研究院), 准教授 (20422511)
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Project Period (FY) |
2011-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥23,010,000 (Direct Cost: ¥17,700,000、Indirect Cost: ¥5,310,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥11,440,000 (Direct Cost: ¥8,800,000、Indirect Cost: ¥2,640,000)
Fiscal Year 2011: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
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Keywords | 細胞老化 / プログラム細胞死 / 花弁老化 / 花持ち性 / アサガオ / 花弁展開 / 次世代シーケンサー / トランスクリプトーム / 開花 / 暗期応答 / 概日時計 / 品種間差異 / エチレン感受性 / 種子生産 / トランスクリプトーム解析 / アセンブラ / 参照配列 / 次世代シークエンサー / de novoアセンブル / 暗期応答遺伝子 / RNAi法 / エチレン / 切り花 |
Outline of Final Research Achievements |
In this study, we performed comparative expression analysis by using RNA-Seq and RT-qPCR methods in the petals of morning glory and revealed that transcription factor gene, InNAP, and ethylene-related genes were associated with the strain difference of flower longevity. We also confirmed that several genes involved in the control of petal senescence by using transgenic plants which reduced expression of 14-3-3 protein gene, InPSR42. In addition, we detected dark-responsive genes, DR03 and DR11, involved with the determination of petal opening time of morning glory by SSH methods.
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Report
(6 results)
Research Products
(16 results)
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[Presentation] InPSR42, a putative 14-3-3 protein, regulates petal opening and senescence in Japanese morning glory2014
Author(s)
Hanako Ono, Kazuo Ishii, Toshinori Kozaki, Keiichi Shimizu, Kenichi Shibuya, Kazuo Ichimura, Motoki Kanekatsu, Tetsuya Yamada
Organizer
The 29th International Horticultural Congress
Place of Presentation
Brisbane Convention & Exhibition Centre (Queensland, Australia)
Year and Date
2014-08-17 – 2014-08-23
Related Report
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