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Identification of osteogenic transcriptional networks by chromatin immunoprecipitation sequencing and genetic approaches

Research Project

Project/Area Number 23689079
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Surgical dentistry
Research InstitutionThe University of Tokyo

Principal Investigator

OHBA Shinsuke  東京大学, 工学(系)研究科(研究院), 准教授 (20466733)

Project Period (FY) 2011-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥27,170,000 (Direct Cost: ¥20,900,000、Indirect Cost: ¥6,270,000)
Fiscal Year 2013: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
Fiscal Year 2012: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Fiscal Year 2011: ¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000)
Keywords骨芽細胞 / 転写 / 骨形成 / 転写ネットワーク
Outline of Final Research Achievements

This study aims to determine molecular mechanisms through which transcriptional regulators that are required for skeletal formation regulate differentiation of skeletal progenitors. Chromatin immunoprecipitation sequencing (ChIP-seq) and expression profiling in mouse primary osteoblasts and chondrocytes identified binding regions and target genes of the regulators in a genome-wide manner. Bioinformatic analyses of the data set provided genome-wide evidences for modes of action of each factor and its biological significance. These findings may help us understand mechanisms underlying the regulation of skeletal formation and lead to basic knowledge to achieve skeletal regenerative medicine.

Report

(4 results)
  • 2014 Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (20 results)

All 2014 2013 2012 2011 Other

All Journal Article (8 results) (of which Peer Reviewed: 8 results,  Open Access: 2 results,  Acknowledgement Compliant: 2 results) Presentation (7 results) (of which Invited: 1 results) Book (1 results) Remarks (4 results)

  • [Journal Article] Stepwise differentiation of pluripotent stem cells into osteoblasts using four small molecules under serum-free and feeder-free conditions.2014

    • Author(s)
      Kanke K, Masaki H, Saito T, Komiyama Y, Hojo H, Nakauchi H, Lichtler AC, Takato T, Chung UI, Ohba S
    • Journal Title

      Stem Cell Reports.

      Volume: 6 Issue: 6 Pages: 751-760

    • DOI

      10.1016/j.stemcr.2014.04.016

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Gli1 haploinsufficiency leads to decreased bone mass with an uncoupling of bone metabolism in adult mice.2014

    • Author(s)
      Kitaura Y, Hojo H, Komiyama Y, Takato T, Chung UI, Ohba S
    • Journal Title

      PLoS ONE

      Volume: 9(10) Issue: 10 Pages: e109597-e109597

    • DOI

      10.1371/journal.pone.0109597

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Gene regulatory networks mediating canonical Wnt signal directed control of pluripotency and differentiation in embyo stem cells.2013

    • Author(s)
      Xiaoxiao Zhang
    • Journal Title

      Stem Cells

      Volume: - Issue: 12 Pages: 2667-2679

    • DOI

      10.1002/stem.1371

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bioactive factors for tissue regeneration: State of Art.2012

    • Author(s)
      Shinsuke Ohba
    • Journal Title

      Muscles, Ligaments and Tendons Journal

      Volume: 2 Pages: 193-203

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Current progress on tissue engineering of bone and cartilage.2012

    • Author(s)
      Shinsuke Ohba
    • Journal Title

      Endocrinology and Metabolism

      Volume: 27 Issue: 1 Pages: 1-11

    • DOI

      10.3803/enm.2012.27.1.1

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Gli1 protein participates in Hedgehog-mediated specification of osteoblast lineage during endochondral ossification2012

    • Author(s)
      Hojo H, 他
    • Journal Title

      J Biol Chem

      Volume: 287 Issue: 21 Pages: 17860-17869

    • DOI

      10.1074/jbc.m112.347716

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Famotidine suppresses osteogenic differentiation of tendon cells in vitro and pathological calcification of tendon in vivo2012

    • Author(s)
      Yamamoto K, Hojo H, 他
    • Journal Title

      J Orthop Res

      Volume: 30 Issue: 12 Pages: 1958-1962

    • DOI

      10.1002/jor.22146

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Current Progress on Tissue Engineering of Bone and Cartilage2012

    • Author(s)
      Shinsuke Ohba
    • Journal Title

      Endocrinology and Metabolism

      Volume: (In press)(印刷中)(掲載確定)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] Identification of distinct modes of Sox9 action in chondrocytes.2014

    • Author(s)
      Shinsuke Ohba
    • Organizer
      4th International SOX Research Conference
    • Place of Presentation
      Cleveland, OH, USA
    • Year and Date
      2014-09-11
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 軟骨細胞ゲノムにおけるSox9作動様式の解析2014

    • Author(s)
      大庭伸介
    • Organizer
      第15回運動器科学研究会
    • Place of Presentation
      ベルサール三田、東京
    • Year and Date
      2014-09-05
    • Related Report
      2013 Annual Research Report
  • [Presentation] Gli1 Participates in the Indian Hedgehog-mediated Osteogenesis during Endochondral Ossification.2012

    • Author(s)
      Hironori Hojo
    • Organizer
      34th Annual Meeting of the American Society for Bone and Mineral Research
    • Place of Presentation
      Minneapolis, MN, USA
    • Related Report
      2012 Annual Research Report
  • [Presentation] Osteo-chondrogenic Function of BMP is Directed Toward Osteogenesis by Hh-Gli1 in the Perichondrium.2012

    • Author(s)
      Hironori Hojo
    • Organizer
      34th Annual Meeting of the American Society for Bone and Mineral Research
    • Place of Presentation
      Minneapolis, MN, USA
    • Related Report
      2012 Annual Research Report
  • [Presentation] Development of a Novel Tetrapod-shaped Drug-eluting Artificial Bone.2012

    • Author(s)
      Yujiro Maeda
    • Organizer
      34th Annual Meeting of the American Society for Bone and Mineral Research
    • Place of Presentation
      Minneapolis, MN, USA
    • Related Report
      2012 Annual Research Report
  • [Presentation] Understanding the Regulatory Networks in Chondrocyte Development.2012

    • Author(s)
      Xinjun He
    • Organizer
      Cold Spring Harbor Asia Conferences Bone and Cartilage: from Development to Human Diseases
    • Place of Presentation
      Suzhou, China
    • Related Report
      2012 Annual Research Report
  • [Presentation] 遺伝学的手法と組み合わせたクロマチン免疫沈降-シーケンス法の骨軟骨生物学への応用2011

    • Author(s)
      大庭伸介
    • Organizer
      日本動物細胞工学会
    • Place of Presentation
      東京大学山上会館(東京都)
    • Year and Date
      2011-07-22
    • Related Report
      2011 Annual Research Report
  • [Book] 多能性幹細胞を使った骨再生, 再生医療叢書 6 骨格系2012

    • Author(s)
      大庭伸介
    • Total Pages
      178
    • Publisher
      朝倉書店
    • Related Report
      2012 Annual Research Report
  • [Remarks] 東京大学大学院工学系研究科バイオエンジニアリング専攻 教員紹介

    • URL

      http://www.bioeng.t.u-tokyo.ac.jp/faculty/36_ohba.html

    • Related Report
      2013 Annual Research Report
  • [Remarks] 東京大学大学院工学系研究科

    • URL

      http://www.t.u-tokyo.ac.jp/tpage/

    • Related Report
      2012 Annual Research Report
  • [Remarks] 東京大学鄭雄一研究室

    • URL

      http://www.tetrapod.t.u-tokyo.ac.jp/med/index.html

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.tetrapod.t.u-tokyo.ac.jp/med/index.html

    • Related Report
      2011 Annual Research Report

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Published: 2011-04-06   Modified: 2019-07-29  

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