Genetic analysis of physiological function of Rac1 and Cdc42 in dendritic spines

• Project/Area Number: 23700368
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neuroscience in general
• Research Institution: The University of Tokyo
• Principal Investigator: KASSAI Hidetoshi 東京大学, 大学院・医学系研究科, 助教 (40403232)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 遺伝子操作マウス / Rac1 / Cdc42 / 神経細胞 / 大脳皮質 / 細胞骨格 / mTOR

Research Abstract

In this study, I focused on analysis of neuronal functions of Rho family GTPase, Rac1 and Cdc42. We adopted analyses using both region-specific knockout mice and brain slice culture, and revealed that Rac1 is involved in the regulation of spine formation and/or morphogenesis in the cerebral cortex andhippocampus. These results may provide insights into human diseases such as mental retardation.

Research Products

• [Journal Article] Functional coupling of the metabotropic glutamate receptor, InsP3 receptor and L-type Ca^2+ channel in mouse CA1pyramidal cells. (2012)
  • Author(s): Kato HK, Kassai H, Watabe AM, Aiba A, Manabe T
  • Journal Title: J. Physiol Volume: 590 Pages: 3019-3034
  • Peer Reviewed
• [Journal Article] Cdc42 isrequired for chondrogenesis and interdigital programmed cell death during limb development. (2012)
  • Author(s): Aizawa R, Yamada A, Suzuki D, Iimura T, Kassai H, Harada T, Tsukasaki M, Yamamoto G, Tachikawa T, Nakao K, Yamamoto M, Yamaguchi A, Aiba A, Kamijo R
  • Journal Title: Mech. Dev Volume: 129 Pages: 38-50
  • Peer Reviewed
• [Journal Article] Functional coupling of the metabotropic glutamate receptor, InsP3 receptor and L-type Ca2+ channel in mouseCA1 pyramidal cells. (2012)
  • Author(s): Kato HK, Kassai H, Watabe AM, Aiba A, Manabe T
  • Journal Title: Journal of Physiology Volume: 590 Issue: 13 Pages: 3019-3034
  • DOI: 10.1113/jphysiol.2012.232942
  • Peer Reviewed
• [Journal Article] Cdc42 is required for chondrogenesis and interdigital programmed cell death during limb development (2011)
  • Author(s): Aizawa R, Yamada A, Suzuki D, Iimura T, Kassai H, Harada T, Tsukasaki M, Yamamoto G, Tachikawa T, Nakao K, Yamamoto M, Yamagchi A, Aiba A, Kamijo R
  • Journal Title: AMech Dev Volume: (In press) Issue: 1-4 Pages: 38-50
  • DOI: 10.1016/j.mod.2012.02.002
  • Peer Reviewed
• [Presentation] Genetic manipulation of mTOR signaling in mouse cerebral cortex (2012)
  • Author(s): Hidetoshi Kassai
  • Organizer: 42nd annual meeting of the Society forNeuroscience
  • Place of Presentation: New Oleans, USA
  • Year and Date: 2012-10-17
• [Presentation] Genetic manipulation of mTOR signaling in mouse cerebral cortex (2012)
  • Author(s): 葛西秀俊
  • Organizer: 42nd annual meeting of the Society for Neuroscience
  • Place of Presentation: アメリカ、ニューオーリンズ
• [Presentation] 大脳皮質形成におけるmTORシグナリングの機能解析 (2011)
  • Author(s): 葛西秀俊
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜市・パシフィコ横浜
  • Year and Date: 2011-12-16
• [Presentation] 大脳皮質形成におけるmTORシグナリングの機能解析 (2011)
  • Author(s): 葛西 秀俊
  • Organizer: 第34回 日本分子生物学会年会
  • Place of Presentation: 神奈川県・パシフィコ横浜
• [Book] 「mTORシグナルによる脳機能の調節と破綻」細胞工学 (2012)
  • Author(s): 葛西秀俊,饗場篤
  • Publisher: 学研メディカル秀潤社
• [Book] 細胞工学（ターゲット・オブ・mTOR、mTORシグナルによる脳機能の調節と破綻） (2012)
  • Author(s): 葛西秀俊
  • Publisher: 秀潤社
• [Book] Farnesylation versus geranylgeranylation in G-protein-mediated light signaling. (2011)
  • Author(s): Hidetoshi Kassai, Yoshitaka Fukada
  • Publisher: The Enzymes
• [Book] The Enzymes, Protein Prenylation PART A (2011)
  • Author(s): Hidetoshi Kassai
  • Publisher: Elsevier
• [Remarks]
  • URL: http://lar.cdbim.m.u-tokyo.ac.jp/
• [Remarks] 東京大学医学部・疾患生命工学センター・動物資源学部門
  • URL: http://lar.cdbim.m.u-tokyo.ac.jp/