| Project/Area Number |
23701060
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor biology
|
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| Research Institution | University of Miyazaki (2012)
Kumamoto University (2011) |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | C. elegans / 癌 / 幹細胞 / 糖鎖 / 腫瘍 / 分化 |
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| Research Abstract |
Notch signaling is important for differentiation of germ-line stem cells (GSCs) in Caenorhabditis elegans. Upon depletion of the core components of the Notch signaling pathway, GSCs undergo abnormal mitosis and resulted in germlinetumor. In addition, other phenotypes, such as squashed vulva (Sqv), embryonic lethal (Emb) and decreased number of germ cells were also observed. According to our RNAi experiments of 145 human glycosyltransferase in C. elegans, similar phenotype was induced by knockdown of glycosaminoglycan synthases. These results strongly suggest the importance of glycosaminoglycans in the Notch signaling pathway in worms, andthe GSCs of C. eleganshave proven to be a good model for studying the mechanisms underlying stem cell differentiation and tumorigenesis.
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