Development of cancer vaccine therapy based on the control of regulatory T cells

• Project/Area Number: 23701075
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Tumor immunology
• Research Institution: Wakayama Medical University
• Principal Investigator: MIYAZAWA Motoki 和歌山県立医科大学, 医学部, 学内助教 (90549734)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: regulatory T cell / VEGFR2 / Regulatory T cell / 癌免疫療法 / 制御性T細胞

Research Abstract

The proportion of Tregs in the peripheral blood decreased in patients with pancreatic cancer who administered VEGFR2-peptide and had positive VEGFR2-specific CTL responses(Cancer Sci. 2010). We hypothesized that VEGFR2-specific CTL had cytotoxicity against Tregs overexpressing VEGFR2(Eur.J.Immunol. 2010). However, this study showed that there was no difference in the cytotoxicity of VEGFR2-specific CTL against Tregs with or without VEGFR2 expression. It is necessary to investigate the correlation between the VEGFR2-specific cytotoxicity against Tregs expressing VEGFR2 and the expression of MHC Class I on the Tregs for the future.

Research Products

• [Journal Article] Indication for the use of an interposed graft during portal vein and/or superior mesenteric vein reconstruction in pancreatic resection based on perioperative outcomes (2014)
  • Author(s): Hirono S, Kawai M, Tani M, Okada K, Miyazawa M, Shimizu A, Kitahata Y, Yamaue H
  • Journal Title: Langenbecks Arch Surg. Volume: 399(4) Pages: 461-71
  • Peer Reviewed
• [Journal Article] Preoperative cholangitis during biliary drainage increases the incidence of postoperative severe complications after pancreaticoduodenectomy (2014)
  • Author(s): Kitahata Y, Kawai M, Tani M, Hirono S, Okada KI, Miyazawa M, Shimizu A, Yamaue H
  • Journal Title: Am J Surg. Volume: Jan 17. pii: S0002-9610(14) [Epubahead of print] Pages: 23-3
  • Peer Reviewed
• [Journal Article] Identification of an HLA-A2-restricted epitope peptide derived from hypoxia-inducible protein 2 (HIG2) (2014)
  • Author(s): Yoshimura S, Tsunoda T, Osawa R, Harada M, Watanabe T, Hikichi T, Katsuda M, Miyazawa M, Tani M, Iwahashi M, Takeda K, Katagiri T, Nakamura Y, Yamaue H
  • Journal Title: PLoS One. Volume: 9(1) eCollection 2014
  • Peer Reviewed
• [Journal Article] Phase II clinical study of alternate-day oral therapy with S-1 as first-line chemotherapy for locally advanced and metastatic pancreatic cancer (2014)
  • Author(s): Yamaue H, Satoi S, Kanbe T, Miyazawa M, Tani M, Kawai M, Hirono S, Okada K, Yanagimoto H, Kwon AH, Mukouyama T, Tsunoda H, Chijiiwa K, Ohuchida J, Kato J, Ueda K, Yamaguchi T, Egawa S, Hayashi K, Shirasaka T
  • Journal Title: Cancer Chemother Pharmacol. Volume: 73(1) Pages: 97-102
  • Peer Reviewed
• [Journal Article] Isolated Roux-en-Y anastomosis of the pancreatic stump in a duct-to-mucosa fashion in patients with distal pancreatectomy with en-bloc celiac axis resection (2014)
  • Author(s): Okada K, Kawai M, Tani M, Hirono S, Miyazawa M, Shimizu A, Kitahata Y, Yamaue H
  • Journal Title: J Hepatobiliary Pancreat Sci. Volume: 21(3) Pages: 193-8
  • Peer Reviewed
• [Journal Article] Antitumor immune response of dendritic cells (DCs) expressing tumor-associated antigens derived from induced pluripotent stem cells : in comparison to bone marrow-derived DCs (2014)
  • Author(s): Iwamoto H, Ojima T, Hayata K, Katsuda M, Miyazawa M, Iida T, Nakamura M, Nakamori M, Iwahashi M, Yamaue H
  • Journal Title: Int J Cancer. Volume: 134(2) Pages: 332-41
  • Peer Reviewed
• [Journal Article] Cancer vaccine therapy using genetically modified induced pluripotent stem cell-derived dendritic cells expressing the TAA gene (2013)
  • Author(s): Iwamoto H, Ojima T, Nakamori M, Nakamura M, Hayata K, Katsuda M, Iida T, Miyazawa M, Iwahashi M, Yamaue H
  • Journal Title: Gan To Kagaku Ryoho. Volume: 40(12) Pages: 1575-7
  • Peer Reviewed
• [Journal Article] Current topics and future to vaccine for pancreatic cancer (2013)
  • Author(s): Miyazawa M, Yamaue H
  • Journal Title: Nihon Jibiinkoka Gakkai Kaiho. Volume: May;116(5) Review Pages: 573-80
  • Peer Reviewed
• [Journal Article] Stump closure of a thick pancreas using stapler closure increases pancreatic fistula after distal pancreatectomy (2013)
  • Author(s): Kawai M, Tani M, Okada K, Hirono S, Miyazawa M, Shimizu A, Kitahata Y, Yamaue H
  • Journal Title: Am J Surg. Volume: 206(3) Pages: 352-9
  • Peer Reviewed
• [Journal Article] Inhibition of IL-17A in tumor microenvironment augments cytotoxicity of tumor-infiltrating lymphocytes in tumor-bearing mice (2013)
  • Author(s): Hayata K, Iwahashi M, Ojima T, Katsuda M, Iida T, Nakamori M, Ueda K, Nakamura M, Miyazawa M, Tsuji T, Yamaue H
  • Journal Title: PLoS One. Volume: 8(1)
  • Peer Reviewed
• [Journal Article] Surgical strategy for patients with pancreatic body/tail carcinoma : who should undergo distal pancreatectomy with en-bloc celiac axis resection? (2013)
  • Author(s): Okada K, Kawai M, Tani M, Hirono S, Miyazawa M, Shimizu A, Kitahata Y, Yamaue H
  • Journal Title: Surgery Volume: 153(3) Pages: 365-72
  • Peer Reviewed
• [Journal Article] Current topics and future to vaccine for pancreatic cancer (2013)
  • Author(s): Miyazawa M, Yamaue H
  • Journal Title: Nihon Jibiinkoka Gakkai Kaiho Volume: 116 Pages: 573-80
• [Journal Article] Identification of HLA-A24-restricted novel T Cell epitope peptides derived from P-cadherin and kinesin family member 20A (2012)
  • Author(s): Osawa R, Tsunoda T, Yoshimura S, Watanabe T, Miyazawa M, Tani M, Takeda K, Nakagawa H, Nakamura Y, Yamaue H
  • Journal Title: J Biomed Biotechnol. Volume: 2012 Pages: 848042-848042
  • Peer Reviewed
• [Journal Article] Identification of the lymphatic drainage pathways from the pancreatic head guided by indocyanine green fluorescence imaging during pancreaticoduodenectomy (2012)
  • Author(s): Hirono S, Tani M, Kawai M, Okada K, Miyazawa M, Shimizu A, Uchiyama K, Yamaue H
  • Journal Title: Dig Surg. Volume: 29(2) Pages: 132-9
  • Peer Reviewed
• [Journal Article] Coexpression of MUC16 and mesothelin is related to the invasion process in pancreatic ductal adenocarcinoma (2012)
  • Author(s): Shimizu A, Hirono S, Tani M, Kawai M, Okada K, Miyazawa M, Kitahata Y, Nakamura Y, Noda T, Yokoyama S, Yamaue H
  • Journal Title: Cancer Sci. Volume: 103(4) Pages: 739-46
  • Peer Reviewed
• [Journal Article] The carcinoembryonic antigen level in pancreatic juice and mural nodule size are predictors of malignancy for branch duct type intraductal papillary mucinous neoplasms of the pancreas (2012)
  • Author(s): Hirono S, Tani M, Kawai M, Okada K, Miyazawa M, Shimizu A, Kitahata Y, Yamaue H
  • Journal Title: Ann Surg. Volume: 255(3) Pages: 517-22
  • Peer Reviewed
• [Journal Article] Pancreatic dissection in the procedure of pancreaticoduodenectomy (with videos) (2012)
  • Author(s): Yamaue H, Tani M, Kawai M, Hirono S, Okada K, Miyazawa M
  • Journal Title: J Hepatobiliary Pancreat Sci. Volume: Mar;19(2) Pages: 95-9
  • NAID: 10030547396
  • Peer Reviewed
• [Journal Article] 最新の膵癌免疫治療 (2012)
  • Author(s): 宮澤基樹, 他
  • Journal Title: 外科 Volume: 74 Pages: 2012-2015
• [Journal Article] 膵癌に対する癌ペプチドワクチン療法の開発 (2012)
  • Author(s): 宮澤基樹, 他
  • Journal Title: 和歌山医学 Volume: 63 Pages: 21-19
  • NAID: 40019277906
  • Peer Reviewed
• [Journal Article] 最新の膵癌免疫治療 (2012)
  • Author(s): 宮澤基樹 山上裕機
  • Journal Title: 外科 Volume: 74巻 Pages: 2012-2015
• [Journal Article] 膵癌に対する癌ペプチドワクチン療法の開発 (2012)
  • Author(s): 宮澤基樹
  • Journal Title: 和歌山医学 Volume: 63巻 Pages: 21-29
  • NAID: 40019277906
• [Journal Article] Peptide vaccine therapy with TLR-9 agonist for patients with esophageal squamous cell carcinoma (2011)
  • Author(s): Katsuda M, Iwahashi M, Matsuda K, Miyazawa M, Nakamori M, Nakamura M, Naka T, Ojima T, Iida T, Yamaue H
  • Journal Title: Gan To Kagaku Ryoho Volume: 38(12) Pages: 1942-4
  • Peer Reviewed
• [Journal Article] The development of a novel cancer vaccine using Peptide vaccine for patients with advanced pancreatic cancer (2011)
  • Author(s): Miyazawa M, Yamaue H
  • Journal Title: Gan To Kagaku Ryoho Volume: Nov;38(12) Pages: 1903-5
  • Peer Reviewed
• [Journal Article] Comparison of different classes of CpG-ODN in augmenting the generation of human epitope peptide-specific CTLs (2011)
  • Author(s): Katsuda M, Iwahashi M, Matsuda K, Miyazawa M, Nakamori M, Nakamura M, Ojima T, Iida T, Hayata K, Yamaue H
  • Journal Title: Int J Oncol. Volume: 39(5) Pages: 1295-302
  • Peer Reviewed
• [Journal Article] Influence of visceral obesity for postoperative pulmonary complications after pancreaticoduodenectomy (2011)
  • Author(s): Shimizu A, Tani M, Kawai M, Hirono S, Miyazawa M, Uchiyama K, Yamaue H
  • Journal Title: J Gastrointest Surg. Volume: 15(8) Pages: 1401-10
  • Peer Reviewed
• [Journal Article] ペプチドワクチンを用いた免疫療法4)抗がん剤との併用 (2011)
  • Author(s): 宮澤基樹 山上裕機
  • Journal Title: 腫瘍内科 Volume: 8 Pages: 439-445
• [Presentation] 膵癌術後補助療法としての癌ペプチドワクチンの開発 (2013)
  • Author(s): 宮澤基樹, 他
  • Organizer: 第26回日本バイオセラピィ学会
  • Place of Presentation: 岩手県民情報交流センターアイーナ(岩手)
  • Year and Date: 2013-12-06
• [Presentation] 膵癌に対する腫瘍抗原遺伝子導入樹状細胞ワクチン療法の基礎的研究 (2013)
  • Author(s): 宮澤基樹, 他
  • Organizer: 第44回日本膵臓学会
  • Place of Presentation: 仙台国際センター(宮城)
  • Year and Date: 2013-07-25
• [Presentation] 切除後膵癌に対する再発予防のためのがんペプチドワクチン療法の開発 (2013)
  • Author(s): 宮澤基樹, 他
  • Organizer: 第68回日本消化器外科学会
  • Place of Presentation: シーガイヤコンベンションセンター(宮崎)
  • Year and Date: 2013-07-19
• [Presentation] 切除後膵癌に対する再発予防のためのがんペプチドワクチン療法の開発 (2013)
  • Author(s): 宮澤基樹
  • Organizer: 第68回日本消化器外科学会総会
  • Place of Presentation: シーガイヤコンベンションセンター（宮崎県）
• [Presentation] 膵癌に対する腫瘍抗原遺伝子導入樹状細胞ワクチン療法の基礎的研究 (2013)
  • Author(s): 宮澤基樹
  • Organizer: 第44回日本膵臓学会大会
  • Place of Presentation: 仙台国際センター（宮城県）
• [Presentation] 膵癌術後補助療法としての癌ペプチドワクチンの開発 (2013)
  • Author(s): 宮澤基樹
  • Organizer: 第26回日本バイオセラピィ学会
  • Place of Presentation: 岩手県民情報交流センター アイーナ（岩手県）
• [Presentation] Dendritic cells adenovirally-transduced with full-length mesothelin cDNA elicit mesothelin-specific cytotoxicity against pancreatic cancer cell lines in vitro (2012)
  • Author(s): Miyazawa et al
  • Organizer: Pancreas Cancer 2012 Kyoto
  • Place of Presentation: International Conference Center (kyoto)
  • Year and Date: 2012-10-06
• [Presentation] How do we predict the clinical relevant pancreatic fistula after pancreaticoduodenectomy? (2012)
  • Author(s): Miyazawa et al
  • Organizer: An analysis in 244 consecutive patients-10th World Congress of the International Hepato-Pancreato-Biliary Association
  • Place of Presentation: Palais des Congres (Paris)
  • Year and Date: 2012-07-01
• [Presentation] 消化器癌免疫療法の開発と免疫動態解析 (2012)
  • Author(s): 宮澤基樹, 他
  • Organizer: 第22回日本サイトメトリー学会
  • Place of Presentation: 千里ライフサイエンスセンター(大阪)
  • Year and Date: 2012-06-29
• [Presentation] 膵癌術後再発予防のための癌ペプチドワクチンの開発 (2012)
  • Author(s): 宮澤基樹, 他
  • Organizer: 第33回癌免疫外科研究会
  • Place of Presentation: 新横浜プリンスホテル(神奈川)
  • Year and Date: 2012-05-17
• [Presentation] 切除不能膵癌に対する癌ペプチドワクチン療法の開発-癌免疫機構を応用した分子標的治療- (2012)
  • Author(s): 宮澤基樹, 他
  • Organizer: 第112回日本外科学会
  • Place of Presentation: 幕張メッセ(千葉)
  • Year and Date: 2012-04-13
• [Presentation] 切除不能膵癌に対する癌ペプチドワクチン療法の開発-癌免疫機構を応用した分子標的治療- (2012)
  • Author(s): 宮澤基樹
  • Organizer: 第112回日本外科学会
  • Place of Presentation: 千葉県千葉市
• [Presentation] 膵癌術後再発予防のための癌ペプチドワクチンの開発 (2012)
  • Author(s): 宮澤基樹
  • Organizer: 第33回癌免疫外科研究会
  • Place of Presentation: 神奈川県横浜市
• [Presentation] 消化器癌免疫療法の開発と免疫動態解析 (2012)
  • Author(s): 宮澤基樹
  • Organizer: 第22回日本サイトメトリー学会学術集会
  • Place of Presentation: 大阪府豊中市
• [Presentation] How do we predict the clinical relevant pancreatic fistula after pancreaticoduodenectomy? – An analysis in 244 consecutive patients – (2012)
  • Author(s): 宮澤基樹
  • Organizer: 10th World Congress of the International Hepato-Prancreato-Biliary Association
  • Place of Presentation: Paris, France
• [Presentation] Dendritic cells adenovirally-transduced with full-length mesothelin cDNA elicit mesothelin-specific cytotoxicity against pancreatic cancer cell lines in vitro (2012)
  • Author(s): 宮澤基樹
  • Organizer: Pancreas Cancer 2012
  • Place of Presentation: 京都府京都市
• [Presentation] 膵癌に対する癌ペプチドワクチン療法の多施設共同試験の現状と展望 (2011)
  • Author(s): 宮澤基樹, 他
  • Organizer: 日本バイオセラピィ学会
  • Place of Presentation: ダイワロイネットホテル(和歌山)
  • Year and Date: 2011-12-02
• [Presentation] The development of cancer vaccine using peptide vaccine for patients with advanced pancreatic cancer (2011)
  • Author(s): 宮澤基樹, 他
  • Organizer: AOPA&KPBA
  • Place of Presentation: Lotte Hotel(韓国)
  • Year and Date: 2011-09-03
• [Presentation] Peptide vaccine therapy for patients with advanced pancreatic cancer (2011)
  • Author(s): 宮澤基樹, 他
  • Organizer: International Surgical Week
  • Place of Presentation: パシフィコ横浜(神奈川)
  • Year and Date: 2011-08-28
• [Presentation] 消化器癌ペプチドワクチン療法におけるフローサイトメトリーを用いた免疫動態解析 (2011)
  • Author(s): 宮澤基樹, 他
  • Organizer: 日本サイトメトリー学会
  • Place of Presentation: 京都市国際交流会館(京都)
  • Year and Date: 2011-06-26
• [Presentation] 消化器癌ペプチドワクチン療法におけるフローサイトメトリーを用いた免疫動態解析 (2011)
  • Author(s): 宮澤基樹
  • Organizer: 日本サイトメトリー学会
  • Place of Presentation: 京都市国際交流会館（京都）
• [Presentation] 膵癌に対する癌ペプチドワクチン療法の多施設共同試験の現状と展望 (2011)
  • Author(s): 宮澤基樹
  • Organizer: 日本バイオセラピィ学会
  • Place of Presentation: ダイワロイネットホテル（和歌山）
• [Presentation] The development for a novel cancer vaccine using peptide vaccine for patients with advanced pancreatic cancer (2011)
  • Author(s): 宮澤基樹
  • Organizer: AOPA & KPBA
  • Place of Presentation: Emerald Room, Lotte Hotel（韓国）
• [Presentation] Peptide vaccine therapy for patients with advanced pancreatic cancer (2011)
  • Author(s): 宮澤基樹
  • Organizer: International Surgical Week
  • Place of Presentation: パシフィコ横浜（神奈川）
• [Book] 専門医のための消化器病学 (2013)
  • Author(s): 宮澤基樹, 他
  • Publisher: 医学書院
• [Book] 専門医のための消化器病学 (2013)
  • Author(s): 宮澤基樹 山上裕機 他
  • Total Pages: 686
  • Publisher: 医学書院