Development of new diagnostic method using antisense RNA of the peripheral blood

• Project/Area Number: 23701081
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Tumor diagnosis
• Research Institution: University of Tsukuba
• Principal Investigator: KOBAYASHI Akihiko 筑波大学, 医学医療系, 講師 (10446552)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: アンチセンスRNA / 癌 / スクリーニング

Research Abstract

The purpose of this research is to develop a new diagnostic method by analyzing blood antisense RNA. Blood samples of 28 colon cancer patients and 6 healthy volunteers were collected and custom made microarray of blood RNA was performed. 20 sets of antisense RNAs were distinguished between colon cancer and healthy persons. By detecting antisense RNA of peripheral blood RNA is a useful tool for diagnosing colon cancer.

Research Products

• [Journal Article] Invasion of the hepatic artery is a crucial predictor of poor outcomes in gallbladder carcinoma (2012)
  • Author(s): Kobayashi A, Oda T, Fukunaga K, Sasaki R, Ohkohchi N
  • Journal Title: World J Surg Volume: 36 Pages: 645-650
  • Peer Reviewed
• [Journal Article] Diffarential expression profiles of sense and antisense transcripts between HCV-associated hepatocellular carcinoma and corresponding non-cancerous livertissue (2012)
  • Author(s): Nagai K, Kohno K, Chiba M, Pak S, Murata S, Fukunaga K, Kobayashi A, Yasue H, Ohkohchi N
  • Journal Title: Int J Oncol Volume: 40 Pages: 1813-1820
  • Peer Reviewed
• [Journal Article] Effective delivery of chemotherapeutic nanoparticles by depleting host kupffer Cells (2012)
  • Author(s): Ohara Y, Oda T, Yamada K, Hashimoto S, Akashi Y, Miyamoto R, Kobayashi A, Fukunaga K, Sasaki R, Ohkohchi N
  • Journal Title: Int J Cancer Volume: 131 Pages: 2402-2410
  • Peer Reviewed
• [Journal Article] Recognition of other organ involvement might assist in the differential diagnosis of IgG 4-associated sclerosing cholangitis without apparent pancreatic involvement: report of two cases (2012)
  • Author(s): Nowatari T, Kobayashi A, Fukunaga K, Oda T, Sasaki R, Ohkohchi N
  • Journal Title: Surg Today Volume: 42 Pages: 1111-1115
  • NAID: 10031120778
  • Peer Reviewed
• [Journal Article] Differential expression profiles of sense and antisense transcripts between HCV-associated hepatocellular carcinoma and corresponding non-cancerous liver tissue. (2012)
  • Author(s): Nagai K
  • Journal Title: International Journal of Oncology Volume: 40 Pages: 1813-1820
  • DOI: 10.3892/ijo.2012.1382
  • Peer Reviewed
• [Journal Article] Invasion of the hepatic artery is a crucial predictor of poor outcomes in gallbladder carcinoma. (2012)
  • Author(s): Kobayashi A
  • Journal Title: World Journal of Surgery Volume: 36 Issue: 3 Pages: 645-650
  • DOI: 10.1007/s00268-011-1413-z
  • Peer Reviewed
• [Presentation] 血液中センス/アンチセンスRNAを用いた大腸癌の新規診断法の開発 (2012)
  • Author(s): 高野恵輔、朴秀吉、永井健太郎、千葉 満、村田聡一郎、久倉勝治、稲川 智、安江博、大河内信弘
  • Organizer: 第112回日本外科学会定期学術集会
  • Place of Presentation: 千葉