| Project/Area Number |
23710003
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Environmental dynamic analysis
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
MIYAJIMA Kumiko 東京医科歯科大学, 生体材料工学研究所, 技術職員 (10516298)
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| Research Collaborator |
MITSUBAYASHI Kohji 東京医科歯科大学, 生体材料工学研究所, 教授 (40307236)
KUDO Hiroyuki 東京医科歯科大学, 生体材料工学研究所, 講師 (70329118)
ARAKAWA Takahiro 東京医科歯科大学, 生体材料工学研究所, 助教 (50409637)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 環境計測 / 光ファイバ / ダニアレルゲン / Der f1 / 蛍光検出 / 光ファイバプローブ / ハウスダスト |
|---|
| Research Abstract |
A fiber-optic immunoassay system based on chemifluorescence detection for Der f1 derived from Dermatophagoides farinae. In this system, a light emitting diode (LED) was used as an excitation light source, and a photomultiplier was used as a fluorescence detector. Capture antibodies were immobilized on the end face of the optical fiber probe, and immunocomplexes were formed and labeled with fluorophores. Calibration range of the system for Der f1 defined by the fluorescence intensities was7.8-250 ng/ml. The study proved that the immunoassay system was useful in the basic technology for the on-site monitoring of environmental allergens.
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