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Automated construction of microtubule networks using MEMS tweezersfor transporting extremely smallouantm

Research Project

Project/Area Number 23710108
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Nanostructural science
Research InstitutionThe University of Tokyo

Principal Investigator

TARHAN Mehmetc  東京大学, 生産技術研究所, 特任研究員 (50582839)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords分子移動システム / 直接分子操作 / イメージプロセス / MEMS ピンセット / 微小管 / MEMSピンセット
Research Abstract

Intracellular transport is based on motor pr,oteei.ng. kinesin,motion along rail structures, e.g. microtubule.s This works aims at buildinignavnitroreconstruction ofthe intracellular transport with singlmeicrotubules filaments to usefor the handling of extremely small amount of analytes in a microfluidic chip. To achieve this goal, MEMS tweezers were used to capture amicsriontgulbeules filament and thenrelocated to a p-dresignated area. Repeating the capturing and relocation sateps, microtubule network could be assembled for kinesin motion to use beads for carryingextremely small amounts of analytes in a chemical detection system.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (7 results)

All 2013 2012 2011

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (5 results)

  • [Journal Article] Specific Transport of Target Molecules by Motor Proteins in Microfluidic Channels2013

    • Author(s)
      M. C. Tarhan, R. Yokokawa, F. O. Morin and H. Fujita
    • Journal Title

      ChemPhysChem

      Volume: Next volume Issue: 8 Pages: 1618-1625

    • DOI

      10.1002/cphc.201300022

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Suspended Microtubules Demonstrate High Sensitivity and Low Experimental Variability in Kinesin Bead Assay2013

    • Author(s)
      M. C. Tarhan, Y. Orazov, R. Yokokawa, S. L. Karsten and H. Fujita
    • Journal Title

      Analyst

      Volume: 138 Issue: 6 Pages: 1653-1656

    • DOI

      10.1039/c3an36545j

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Presentation] Continuous-ReTaimleMonitoring of Moleulcar Detection by Silicon Nanotweezer-sIntegrated Microfluidic Device2012

    • Author(s)
      M. C. Tarha,n D. Collard, L. Jalabert, M. Kumemura, N. Laftiet, Q. Delouvee, S. L.Karsten, H. Fujita
    • Organizer
      16th International Conference on Miniaturized Systems forChemistry and Life Sciences
    • Place of Presentation
      Okinawa, Japan
    • Related Report
      2012 Final Research Report
  • [Presentation] Continuous Real-Time Monitoring of Molecular Detection by Silicon Nanotweezers-Integrated Microfluidic Device2012

    • Author(s)
      M. C. Tarhan, D. Collard, L. Jalabert, M. Kumemura, N. Lafitte, Q. Delouvee, S. L. Karsten and H. Fujita
    • Organizer
      16th International Conference on Miniaturized Systems for Chemistry and Life Sciences
    • Place of Presentation
      Okinawa, Japan
    • Related Report
      2012 Annual Research Report
  • [Presentation] A MOTORPROTEIN-BASED ENZYMATIC DETECTION SYSTEM2011

    • Author(s)
      M. C. Tarha,n R. Yokokawa, L. Jalabert,D. Collard and.HFujita
    • Organizer
      15th International Conference onMiniaturized Systems for Chemistry andLife Sciences
    • Place of Presentation
      Seattle, WA,USA
    • Year and Date
      2011-10-04
    • Related Report
      2012 Final Research Report
  • [Presentation] Molecular Sorting Based on Motor Protein Powered Direct Transport2011

    • Author(s)
      M. C. Tarhan, R. Yokokawa, F. O. Morin and H. Fujita
    • Organizer
      Foundations of Nanoscience Meeting (FNANO)
    • Place of Presentation
      Snowbird, UT, USA
    • Year and Date
      2011-04-12
    • Related Report
      2012 Final Research Report 2011 Research-status Report
  • [Presentation] A MOTOR PROTEIN-BASED ENZYMATIC DETECTION SYSTEM2011

    • Author(s)
      M. C. Tarhan, R. Yokokawa, L. Jalabert, D. Collard and H. Fujita
    • Organizer
      15th International Conference on Miniaturized Systems for Chemistry and Life Sciences
    • Place of Presentation
      Seattle, WA, USA
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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