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Visualization of Mechanical Response of Molecular Machines working in vivo

Research Project

Project/Area Number 23710144
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Nanomaterials/Nanobioscience
Research InstitutionKyoto University

Principal Investigator

NISHIYAMA Masayoshi  京都大学, 白眉センター, 准教授 (10346075)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords分子モーター / 分子機械 / 高圧力顕微鏡 / 水和 / べん毛モーター / 1分子計測 / バイオイメージング / タンパク質 / 高圧力 / バクテリア / タンパク質分子機械 / 1分子計測
Research Abstract

In this research project, we developed a new assay that enables to control the functional expression of molecular machines working in living cells. We constructed a new high-pressure microscope that allows us to modulate the intermolecular interactions between protein and water molecules. By using this system, we were successful to change the rotational direction of Escherichia coli flagellar motors.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (50 results)

All 2014 2013 2012 2011 Other

All Journal Article (15 results) (of which Peer Reviewed: 12 results) Presentation (35 results) (of which Invited: 13 results)

  • [Journal Article] High hydrostatic pressure induces CCW to CW reversals of the Escherichia coli flagellar motor2013

    • Author(s)
      Nishiyama M. et al.
    • Journal Title

      J. Bacteriol.

      Volume: 195(8) Pages: 1809-14

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Single molecule analysis of the rotation of F1-ATPase under high hydrostatic pressure2013

    • Author(s)
      Okuno D., Nishiyama M. & Noji H.
    • Journal Title

      Biophys. J.

      Volume: 105(7) Pages: 1635-42

    • NAID

      120005328380

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] 高圧力顕微鏡2013

    • Author(s)
      西山雅祥 &木村佳文
    • Journal Title

      LTMセンター誌

      Volume: 22 Pages: 18-27

    • NAID

      120005289856

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] バクテリア・べん毛モーターが高圧力下で逆向きに回り出す!?2013

    • Author(s)
      西山雅祥
    • Journal Title

      生物物理

      Volume: 53(5) Pages: 264-5

    • NAID

      10031191850

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] 細胞内の水で生命活動を操る!-高圧力下で観るタンパク質水和変調イメージング2013

    • Author(s)
      西山雅祥 &曽和義幸
    • Journal Title

      化学

      Volume: 68(9) Pages: 31-6

    • NAID

      120005328379

    • Related Report
      2013 Final Research Report
  • [Journal Article] High hydrostatic pressure induces CCW to CW reversals of the Escherichia coli flagellar motor2013

    • Author(s)
      M.Nishiyama, Y.Sowa, Y.Kimura, M. Homma, A. Ishijima, M. Terazima
    • Journal Title

      J.Bacterio.

      Volume: 195 Issue: 8 Pages: 1809-1814

    • DOI

      10.1128/jb.02139-12

    • Related Report
      2013 Annual Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Glycine insertion makes yellow fluorescent protein sensitive to hydrostatic pressure.2013

    • Author(s)
      Watanabe TM, Imada K, Yoshizawa K, Nishiyama M, Kato C, Abe F, Morikawa TJ, Kinoshita M, Fujita H, Yanagida T.
    • Journal Title

      PLoS One

      Volume: 8 Issue: 8 Pages: e73212-e73212

    • DOI

      10.1371/journal.pone.0073212

    • NAID

      120005328377

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Single molecule analysis of the rotation of F1-ATPase under high hydrostatic pressure2013

    • Author(s)
      Daichi Okuno, Masayoshi Nishiyama and Hiroyuki Noji
    • Journal Title

      Biophysical Journal

      Volume: 105 Issue: 7 Pages: 1635-1642

    • DOI

      10.1016/j.bpj.2013.08.036

    • NAID

      120005328380

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 細胞内の水で生命活動を操る! -高圧力下で観るタンパク質水和変調イメージング-2013

    • Author(s)
      西山雅祥, 曽和義幸
    • Journal Title

      化学

      Volume: 68 Pages: 31-36

    • NAID

      120005328379

    • Related Report
      2013 Annual Research Report
  • [Journal Article] High Hydrostatic Pressure Induces Reversal Rotations of the Bacterial Flagellar Motor!?2013

    • Author(s)
      西山雅祥
    • Journal Title

      Seibutsu Butsuri

      Volume: 53 Issue: 5 Pages: 264-265

    • DOI

      10.2142/biophys.53.264

    • NAID

      10031191850

    • ISSN
      0582-4052, 1347-4219
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Microscopic analysis of bacterial motility at high pressure2012

    • Author(s)
      Nishiyama M. & Sowa Y.
    • Journal Title

      Biophys J

      Volume: 102(8) Pages: 1872-80

    • NAID

      120004057183

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Bacterial Motility Measured by a Miniature Chamber for High-Pressure Microscopy2012

    • Author(s)
      Nishiyama M. & Kojima S.
    • Journal Title

      Int. J. Mor. Sci.

      Volume: 13(7) Pages: 9225-39

    • NAID

      120005627663

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] 染色体を牽引する「分子エンジン」2012

    • Author(s)
      西山雅祥
    • Journal Title

      化学

      Volume: 67(1) Pages: 66-7

    • NAID

      120005399094

    • Related Report
      2013 Final Research Report 2011 Research-status Report
  • [Journal Article] Bacterial Motility Measured by a Miniature Chamber for High-Pressure Microscopy2012

    • Author(s)
      Masayoshi NISHIYAMA
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 13 Issue: 7 Pages: 9225-9239

    • DOI

      10.3390/ijms13079225

    • NAID

      120005627663

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Microscopic Analysis of Bacterial Motility at High Pressure2012

    • Author(s)
      Nishiyama M., Sowa Y.
    • Journal Title

      Biophysical Journal

      Volume: 102 Issue: 8 Pages: 1872-1880

    • DOI

      10.1016/j.bpj.2012.03.033

    • NAID

      120004057183

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 高圧力顕微鏡の開発と生体分子機械の運動変調2014

    • Author(s)
      西山雅祥 ,曽和義幸
    • Organizer
      応用物理学会
    • Place of Presentation
      相模原
    • Related Report
      2013 Final Research Report
  • [Presentation] 高圧力を用いたタンパク質分子機械の構造変化イメージング2013

    • Author(s)
      西山雅祥
    • Organizer
      日本機械学会2013年度年次大会
    • Place of Presentation
      岡山
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] 極限環境で駆動する生体ナノマシンの直接観察2013

    • Author(s)
      西山雅祥
    • Organizer
      法政大学ナノテクセミナー
    • Place of Presentation
      法政大
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] タンパク質分子モーターの力学変調と高分解能イメージング2013

    • Author(s)
      西山雅祥
    • Organizer
      東北大学大学院工学研究科応用物理学セミナー
    • Place of Presentation
      仙台
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Dynamic conformational changes of flagellar filament observed by high-pressure microscopy2013

    • Author(s)
      Nishiyama M. & Sowa Y.
    • Organizer
      BLAST XI, "Selected as a Talk"
    • Place of Presentation
      Tucson, USA
    • Related Report
      2013 Final Research Report
  • [Presentation] Dynamic Conformational Changes of Flagellar Filament Observed by High-Pressure Microscopy.2013

    • Author(s)
      Masayoshi NISHIYAMA
    • Organizer
      BLAST XII
    • Place of Presentation
      Tucson, USA
    • Related Report
      2012 Research-status Report
  • [Presentation] Microscopic analysis of bacterial motility at high pressure2012

    • Author(s)
      Nishiyama M.
    • Organizer
      9th International Conference on Flow Dynamics
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] バクテリア遊泳運動の力学変調イメージング2012

    • Author(s)
      西山雅祥
    • Organizer
      法政大学ナノテクセミナー
    • Place of Presentation
      法政大
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] 生物分子モーターの1分子計測と熱ラチェット型運動機構2012

    • Author(s)
      西山雅祥
    • Organizer
      力学的決定性と統計性の中間領域を探る IV
    • Place of Presentation
      関西セミナーハウス(京都)
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] 高圧力下での生体分子機械の運動機能変調イメージング2012

    • Author(s)
      西山雅祥
    • Organizer
      日本機械学会第24回バイオエンジニアリング部門講演会
    • Place of Presentation
      大阪大学
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] ATP駆動型分子モーターの運動変調イメージング2012

    • Author(s)
      西山雅祥
    • Organizer
      特殊環境微生物セミナー
    • Place of Presentation
      海洋研究開発機構・横浜研究所
    • Related Report
      2013 Final Research Report
  • [Presentation] Microscopic analysis of bacterial motility at high pressure2012

    • Author(s)
      Nishiyama M. & Sowa Y.
    • Organizer
      7th International Conference on High Pressure Bioscience and Biotechnology
    • Place of Presentation
      Otsu, Japan
    • Related Report
      2013 Final Research Report
  • [Presentation] 高圧力下で大腸菌の遊泳運動を観察する2012

    • Author(s)
      西山雅祥 ,曽和義幸
    • Organizer
      第9回21世紀大腸菌研究会
    • Place of Presentation
      長浜
    • Related Report
      2013 Final Research Report
  • [Presentation] Bacterial Motility at High Pressure2012

    • Author(s)
      西山雅祥
    • Organizer
      2012年べん毛研究交流会
    • Place of Presentation
      静岡
    • Related Report
      2013 Final Research Report
  • [Presentation] Microscopic analysis of bacterial motility at high pressure2012

    • Author(s)
      Nishiyama M. & Sowa Y.
    • Organizer
      56th Annual Meeting of Biophysical Society,"Selected as a Talk"
    • Place of Presentation
      San Diego, USA
    • Related Report
      2013 Final Research Report
  • [Presentation] 高圧力下にあるバクテリア遊泳運動の阻害機構2012

    • Author(s)
      西山雅祥 ,曽和義幸
    • Organizer
      2012年生体運動研究合同班会議
    • Place of Presentation
      筑波
    • Related Report
      2013 Final Research Report
  • [Presentation] Microscopic Analysis of Bacterial Motility at High Pressure.2012

    • Author(s)
      Masayoshi NISHIYAMA
    • Organizer
      9th International Conference on Flow Dynamics
    • Place of Presentation
      仙台
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 高圧力下で大腸菌の遊泳運動を観察する2012

    • Author(s)
      西山雅祥
    • Organizer
      第9回21世紀大腸菌研究会
    • Place of Presentation
      長浜
    • Related Report
      2012 Research-status Report
  • [Presentation] Microscopic Analysis of Bacterial Motility at High Pressure.2012

    • Author(s)
      Masayoshi NISHIYAMA
    • Organizer
      7th International Conference on High Pressure Bioscience and Biotechnology
    • Place of Presentation
      大津
    • Related Report
      2012 Research-status Report
  • [Presentation] 高圧力下での生体分子機械の運動機能変調イメージング2012

    • Author(s)
      西山雅祥
    • Organizer
      日本機械学会 第24回バイオエンジニアリング部門講演会(招待講演)
    • Place of Presentation
      大阪大学
    • Related Report
      2011 Research-status Report
  • [Presentation] 力学的決定性と統計性の中間領域を探るIV2012

    • Author(s)
      西山雅祥
    • Organizer
      生物分子モーターの1分子計測と熱ラチェット型運動機構(招待講演)
    • Place of Presentation
      関西セミナーハウス(京都)
    • Related Report
      2011 Research-status Report
  • [Presentation] 高圧力下にあるバクテリア遊泳運動の阻害機構2012

    • Author(s)
      西山雅祥 & 曽和義幸
    • Organizer
      2012年生体運動研究合同班会議
    • Place of Presentation
      筑波大学
    • Related Report
      2011 Research-status Report
  • [Presentation] Microscopic Analysis of Bacterial Motility at High Pressure (Platform)2012

    • Author(s)
      Nishiyama M. & Sowa Y.
    • Organizer
      56th Annual Meeting of Biophysical Society
    • Place of Presentation
      San Diego, USA
    • Related Report
      2011 Research-status Report
  • [Presentation] 高圧力下でのバクテリア運動能2012

    • Author(s)
      西山雅祥
    • Organizer
      2011年度べん毛交流会
    • Place of Presentation
      ラフォーレ修繕寺(静岡)
    • Related Report
      2011 Research-status Report
  • [Presentation] High-pressure microscopy for modulating the structure and function of biomolecules2011

    • Author(s)
      Nishiyama M.
    • Organizer
      第49回日本生物物理学会年会
    • Place of Presentation
      姫路
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Microscopic analysis of bacterial motility at high pressure2011

    • Author(s)
      Nishiyama M. & Sowa Y.
    • Organizer
      第49回日本生物物理学会年会
    • Place of Presentation
      姫路
    • Related Report
      2013 Final Research Report
  • [Presentation] High-pressure microscopy reveals the mechanism how applied pressures affect on the bacterial motility2011

    • Author(s)
      Nishiyama M.
    • Organizer
      第49回日本生物物理学会(招待講演)
    • Place of Presentation
      兵庫県立大学
    • Related Report
      2011 Research-status Report
  • [Presentation] 高圧力顕微鏡の開発と生物分子モーターの運動変調イメージング2011

    • Author(s)
      西山雅祥
    • Organizer
      第7回光科学若手研究会(招待講演)
    • Place of Presentation
      大阪大学
    • Related Report
      2011 Research-status Report
  • [Presentation] Pressure-induced reverse rotation in bacterial flagellar motors2011

    • Author(s)
      Nishiyama M.
    • Organizer
      6th International Meeting on Biomolecules under Pressure(招待講演)
    • Place of Presentation
      Otsu City Former Public Hall
    • Related Report
      2011 Research-status Report
  • [Presentation] Microscopic Analysis of Bacterial Motility at High Pressure2011

    • Author(s)
      Nishiyama M. & Sowa Y.
    • Organizer
      第49回日本生物物理学会
    • Place of Presentation
      兵庫県立大学
    • Related Report
      2011 Research-status Report
  • [Presentation] 極限環境で駆動する生体ナノマシンの直接観察

    • Author(s)
      西山雅祥
    • Organizer
      法政大学ナノテクセミナー
    • Place of Presentation
      法政大学
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 高圧力を用いたタンパク質分子機械構造変化イメージング

    • Author(s)
      西山雅祥
    • Organizer
      日本機械学会2013年度大会
    • Place of Presentation
      岡山大学
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 高圧力顕微鏡の開発と生体分子機械の運動変調

    • Author(s)
      西山雅祥, 曽和義幸
    • Organizer
      第61回 応用物理学会 春期学術講演会
    • Place of Presentation
      青山学院大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] バクテリア遊泳運動の力学変調イメージング

    • Author(s)
      西山雅祥
    • Organizer
      法政大学 マイクロ・ナノテクノロジー研究センター 第4回ナノテクセミナー
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] ATP駆動型分子モーターの運動変調イメージング

    • Author(s)
      西山雅祥
    • Organizer
      特殊環境微生物セミナー
    • Place of Presentation
      横浜
    • Related Report
      2012 Research-status Report
    • Invited

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Published: 2011-08-05   Modified: 2019-07-29  

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