Analysis of molecular mechanism of cohesin acetylation by ESCO1 and ESC02

• Project/Area Number: 23710213
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Genome biology
• Research Institution: The University of Tokyo
• Principal Investigator: BANDO Masashige 東京大学, 分子細胞生物学研究所, 助教 (90360627)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: エピゲノム / 染色体構造 / コヒーシン / アセチル化 / ChIP-seq / 姉妹染色体 / ゲノム構造

Research Abstract

We found that Esco1 acetylates cohesin through its N-terminalinteraction with Pds5. ChIP-seq analysis of ESCO1 showed that it co-localizes with cohesinthroughout the cell cycle. On the other hand acetylation of cohesin by ESCO2 requires Mcmhelicases. Thus Esco2 in human, just like Eco1 in yeast, seems to establish sisterchromatids cohesion during DNA replication progression

Research Products

• [Journal Article] Rapid discovery of highly potent and selective inhibitors of histone deacetylase 8 using click chemistry to generate candidate libraries. (2012)
  • Author(s): Suzuki T, Ota Y, Ri M, Bando M, Gotoh A, Itoh Y, Tsumoto H, Tatum PR, Mizukami T, Nakagawa H, Iida S, Ueda R, Shirahige K, Miyata N
  • Journal Title: J Med Chem. Volume: 55 Pages: 9562-9575
  • Peer Reviewed
• [Journal Article] Lombardi PM,Magnaghi-Jaulin L, Mortier GR, Nozaki N, Petersen MB, Seimiya H, Siu VM, Suzuki Y, Takagaki K, Wilde JJ, Willems PJ, Prigent C, Gillessen-Kaesbach G, Christianson DW, Kaiser FJ, Jackson LG, Hirota T,Krantz ID (2012)
  • Author(s): Deardorff MA, Bando M, Nakato R, Watrin E, Itoh T, Minamino M, Saitoh K, Komata M, Katou Y, Clark D, Cole KE, De Baere E, Decroos C, Di Donato N, Ernst S, Francey LJ, Gyftodimou Y, Hirashima K, Hullings M, Ishikawa Y, Jaulin C, Kaur M, Kiyono T
  • Journal Title: Shirahige K..,HDAC8 mutations in Cornelia de Lange syndrome affect the cohesin acetylation cycle., Nature. Volume: 489 Pages: 313-317
  • Peer Reviewed
• [Journal Article] The prereplication complex recruits XEco2 to chromatin to promote cohesin acetylation in Xenopus egg extracts (2012)
  • Author(s): Higashi TL, Ikeda M, Tanaka H, Nakagawa T, Bando M, Shirahige K, Kubota Y, Takisawa H, Masukata H, Takahashi TS
  • Journal Title: Curr Biol Volume: 22 Pages: 977-988
  • Peer Reviewed
• [Journal Article] The Prereplication Complex Recruits XEco2 to Chromatin to Promote Cohesin Acetylation in Xenopus Egg Extracts (2012)
  • Author(s): Torahiko L. Higashi
  • Journal Title: Current Biology Volume: 22 Issue: 11 Pages: 977-988
  • DOI: 10.1016/j.cub.2012.04.013
  • Peer Reviewed
• [Journal Article] HDAC8 mutations in Cornelia de Lange syndrome affect the cohesin acetylation cycle. (2012)
  • Author(s): Deardorff MA
  • Journal Title: Nature Volume: 489(7415) Issue: 7415 Pages: 313-7
  • DOI: 10.1038/nature11316
  • Peer Reviewed