Functional analysis of RAD51 on nucleosomal DNA templates

• Project/Area Number: 23710221
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Genome biology
• Research Institution: Waseda University
• Principal Investigator: TAKAKU Motoki 早稲田大学, 理工学術院, 次席研究員(研究院講師) (30547071)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
• Keywords: ゲノム維持修復 / 相同組換え / ゲノム安定性維持 / DNA修復 / クロマチン構造 / RAD51 / 相同組換え修復 / RAD54 / ヌクレオソーム

Research Abstract

The purpose of this study is to characterize the mechanism of RAD51-mediated homologous pairing on nucleosomal DNA templates. In this study, we established in vitro system to detect homologous paring in nucleosomal DNA templates. We found that the linker histone significantly inhibited the homologous pairing on the nucleosomal DNA. These results suggest that the dynamic action of chromatin may regulate homologous recombination.

Research Products

• [Journal Article] Vertebrate Spt2 is a novel nucleolar histone chaperone that assists in ribosomal DNA transcription (2013)
  • Author(s): Osakabe A, Tachiwana H, Takaku M, Hori T, Obuse C, Kimura H, Fukagawa T, Kurumizaka H
  • Journal Title: Journal of Cell Science Volume: (in press)
  • Peer Reviewed
• [Journal Article] Human PSF concentrates DNA and stimulates duplex capture in DMC1-mediated homologous pairing (2011)
  • Author(s): Morozumi Y, et al
  • Journal Title: Nucleic Acids Res Volume: 40 Issue: 7 Pages: 3031-3041
  • DOI: 10.1093/nar/gkr1229
  • Peer Reviewed
• [Journal Article] Purification of the Human SMN-GEMIN2 Complex and Assessment of Its Stimulation of RAD51-Mediated DNA Recombination Reactions (2011)
  • Author(s): Motoki Takaku, Takashi Tsujita, Naoki Horikoshi, Yoshimasa Takizawa, Yong, Qing, Kouji Hirota, Masae Ikura, Tsuyoshi Ikura, Shunichi Takeda, Hitoshi Kurumizaka
  • Journal Title: Biochemistry Volume: 50 Issue: 32 Pages: 6797-6805
  • DOI: 10.1021/bi200828g
  • Peer Reviewed
• [Journal Article] Biochemical analysis of the human ENA/VASP-family proteins, MENA, VASP and EVL, in homologous recombination (2011)
  • Author(s): Takaku, M., Ueno, H., Kurumizaka, H.
  • Journal Title: J.Biochem. Volume: 149 Issue: 6 Pages: 721-729
  • DOI: 10.1093/jb/mvr029
  • NAID: 10030585933
  • Peer Reviewed
• [Journal Article] Halenaquinone, a chemical compound that specifically inhibits the secondary DNA binding of RAD51 (2011)
  • Author(s): Takaku M, Kainuma T, Ishida-Takaku T, Ishigami S, Suzuki H, Tashiro S, van Soest RWM, Nakao Y, Kurumizaka H
  • Journal Title: Genes Cells Volume: 6 Issue: 4 Pages: 427-436
  • DOI: 10.1111/j.1365-2443.2011.01494.x
  • Peer Reviewed
• [Presentation] 再構成系によるクロマチンでの相同組換え機構の解析 (2012)
  • Author(s): 高久誉大, 町田晋一, 小林航, 林田亮太, 鈴木秀和, 井倉正枝, 越阪部晃永, 立和名博昭, 浦聖恵, 田代聡, 井倉毅, 胡桃坂仁志
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
• [Presentation] Mechanism of the RAD51-mediated homology search on nucleosomal DNA (2012)
  • Author(s): Motoki Takaku, Shinichi Machida, Wataru Kobayashi, Naoki Horikoshi, Wataru Kagawa, Hitoshi Kurumizaka
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] Biochemical studies of homologous pairing on chromatinized DNA (2012)
  • Author(s): Motoki Takaku, Shinichi Machida, Wataru Kobayashi, Ryota Hayashida, Hidekazu Suzuki, Masae Ikura, Akihisa Osakabe, Hiroaki Tachiwana, Satoshi Tashiro, Tsuyoshi Ikura, and Hitoshi Kurumizaka
  • Organizer: The 8th 3R Symposium
  • Place of Presentation: 淡路島
• [Presentation] Biochemical analyses of homologous recombination reaction in chromatin (2012)
  • Author(s): Motoki Takaku, Hitoshi Kurumizaka
  • Organizer: 第71回日本癌学会学術総会,
  • Place of Presentation: 札幌
• [Presentation] Biochemical analyses of homologous recombination reaction in chromatin (2012)
  • Author(s): Motoki Takaku
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌
• [Presentation] RAD51活性制御化合物の探索とその生化学的解析 (2011)
  • Author(s): 高久誉大, 海沼嵩, 石田-高久恭子, 石上進太郎, 鈴木秀和, 田代聡, 木村宏,中尾洋一, 胡桃坂仁志
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2011-09-23
• [Presentation] SMN-GEMIN2 complex stimulates the RAD51-mediated recombination reactions (2011)
  • Author(s): Motoki Takaku, Takashi Tsujita, Naoki Horikoshi, Yoshimasa Takizawa, Yong Qing, Kouji Hirota, Masae Ikura, Tsuyoshi Ikura, Shunichi Takeda, Hitoshi Kurumizaka
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] PLEKHF1 is a novel factor that interacts with the meiosis-specific recombinase, human DMC1 (2011)
  • Author(s): Motoki Takaku, Samika Iwashita, Wataru Kobayashi, Yoshimasa Takizawa, Hitoshi Kurumizaka
  • Organizer: World Congress on Reproductive Biology
  • Place of Presentation: Cairns (Australia)
• [Presentation] SMN-GEMIN2 complex stimulates the RAD51-mediated recombination reactions (2011)
  • Author(s): Motoki Takaku
  • Organizer: 第34回 日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] RAD51活性制御化合物の探索とその生化学的解析 (2011)
  • Author(s): 高久 誉大
  • Organizer: 第84回 日本生化学会大会
  • Place of Presentation: 京都
• [Book] 胡桃坂仁志編"実験医学別冊目的別で選べる核酸実験の原理とプロトコール"(担当項目 : 核酸実験のためのワークフロー,ショ糖勾配遠心法,アルカリ変性法,サルコシルによる非変性法) (2011)
  • Author(s): 平尾一郎
  • Total Pages: 15
  • Publisher: 羊土社
• [Book] 実験医学別冊 目的別で選べる核酸実験の原理とプロトコール (2011)
  • Author(s): 平尾一郎，胡桃坂仁志 編
  • Total Pages: 15
  • Publisher: 羊土社