Molecular mechanism of H2O2 and H2 on Wnt signaling pathway
Project/Area Number |
23710253
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Living organism molecular science
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Research Institution | Nagoya University |
Principal Investigator |
OHKAWARA Bisei 名古屋大学, 高等研究院(医), 特任講師 (80589606)
|
Project Period (FY) |
2011 – 2012
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
|
Keywords | 過酸化水素 / 分子状水素 / Wntシグナル / H2ガス / H2O2 / Wntシグナル伝達経路 / 神経筋接合部 |
Research Abstract |
Hydrogen peroxide (H2O2) is reactive oxygen species (ROS). Molecular hydrogen (H2) is thought to cancel the ROS effects in disease model mice, however the molecular mechanism of H2 remain elusive. Wnt signaling is involved in several phenomena in vivo. Here, for the purpose to know the molecular mechanism of H2O2 and H2 effects, we analyzed the Wnt signaling activity in cells treated with these factors. Within cultured cells, H2 partially cancelled H2O2 effects on Wnt signaling in treatment duration-manner. The results suggest that H2 have effects on the signaling pathway both in H2O2-dependent manner and H2O2–independent manner.
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Report
(4 results)
Research Products
(12 results)
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[Journal Article] LRP4 third β-propeller domain mutations cause novel congenital myasthenia by compromising agrin-mediated MuSK signaling in a position-specific manner2014
Author(s)
Ohkawara B, Cabrera-Serrano M, Nakata T, Milone M, Asai N, Ito K, Ito M, Masuda A, Ito Y, Engel AG, Ohno K
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Journal Title
Hum Mol Genet.
Volume: Apr 1;23(7)
Issue: 7
Pages: 1856-68
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Verapamil protects against cartilage degradation in osteoarthritis by inhibiting Wnt/β-catenin signaling.2014
Author(s)
Takamatsu, A., Ohkawara, B., Ito, M., Masuda, A., Sakai, T., Ishiguro, N. and Ohno, K.
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Journal Title
PLOS ONE
Volume: 9(3)
Issue: 3
Pages: e92699-e92699
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Meclozine facilitates proliferation and differentiation of chondrocytes by attenuating abnormally activated FGFR3 signaling in achondroplasia.2013
Author(s)
Matsushita, M., Kitoh, H., Ohkawara, B., Mishima, K., Kaneko, H., Ito, M., Masuda, A., Ishiguro, N. and Ohno, K.
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Journal Title
PLOS ONE
Volume: 8(12)
Issue: 12
Pages: 81569-81569
DOI
Related Report
Peer Reviewed
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