Analysis of In Situ Chemical Reaction Dynamics by a Multiple Resonance NMR Technique
| Project/Area Number |
23710268
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemical biology
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| Research Institution | Kyoto University |
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Principal Investigator |
YAMADA Hisatsugu 京都大学, 先端医工学研究ユニット, 助教 (80512764)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 細胞内化学反応 / 多重共鳴 NMR / 分子プローブ / NMR イメージング / 多重共鳴NMR / 代謝解析 / NMRイメージング / ケミカルバイオロジー / 安定同位元素集積化 / 代謝反応 / 安定同位体ラベル |
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| Research Abstract |
NMR/MR is one of the most promising techniques for the in situ analysis of specific biochemical (metabolic) reactions, both in vitro and in vivo. In this research, we revealed that (1) one-dimensional triple-resonance NMR is applicable to metabolic analysis of 13C/15N-labeled uracil, (2) integration of stable isotopes into the biocompatible polymer-tag (13C/15N-PMPC) enabled observation of the selective triple resonance NMR signal of 13C/15N-PMPC at a nano-molar level in a mouse liver lysate, and (3) application of a multiple resonance NMR technique to a MR imaging allows us to obtain the selective MR image of 13C/15N-PMPC without endogenous noise signal.
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Report
(3 results)
Research Products
(29 results)
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[Presentation] Triple resonance NMR in application to in situ monitoring of targeted metabolic reaction and its inhibition by a clinical drug2012
Author(s)
Yamada, H.; Mizusawa, K.; Igarashi, R.; Tochio, H.; Shirakawa, M.; Tabata, Y.; Kimura, Y.; Kondo, T.; Aoyama, Y.; Sando, S.
Organizer
244th ACS National Meeting
Place of Presentation
Philadelphia, PA
Year and Date
2012-08-21
Related Report
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[Presentation] Direct In Situ Monitoring of Pyrimidine Catabolism and Its Inhibition Using Triple Resonance NMR Analysis2011
Author(s)
Yamada, H.; Mizusawa, K.; Igarashi, R.; Tochio, H.; Shirakawa, M.; Tabata, Y.; Kimura, Y.; Kondo, T.; Aoyama, Y.; Sando, S
Organizer
38thInternational Symposium on Nucleic Acids Chemistry
Place of Presentation
Hokkaido Univ., Sapporo, Japan
Year and Date
2011-11-10
Related Report
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[Presentation] Direct In Situ Monitoring of Pyrimidine Catabolism and Its Inhibition Using Triple Resonance NMR Analysis2011
Author(s)
Yamada, H.; Mizusawa, K.; Igarashi, R.; Tochio, H.; Shirakawa, M.; Tabata, Y.; Kimura, Y.; Kondo, T.; Aoyama, Y.; Sando, S.
Organizer
38th International Symposium on Nucleic Acids Chemistry
Place of Presentation
Clark Memorial Student Center, Hokkaido Univ., Sapporo, Japan
Related Report
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