Construction of a photoregulating system for self-assembly proteins

• Project/Area Number: 23750179
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Chemistry related to living body
• Research Institution: Tohoku University
• Principal Investigator: UI Mihoko 東北大学, 多元物質科学研究所, 助教 (30549580)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 光応答性蛋白質 / 自己集合蛋白質 / photoactive yellow protein / alpha-hemolysin / 光制御 / 光受容蛋白質 / PYP / 孔形成蛋白質 / 溶血活性制御 / 生体分子デバイス

Research Abstract

We constructed two types of photo-controlled nano-pore devices based on PYP photoreceptor-fused staphylococcal pore-forming toxin alpha-hemolysin. The both PYP-fused devices had the similar photocycle as wild type PYP and inhibited the transmission efficiency of ions through the sheep red blood cells membrane under light irradiation. These PYP-fused devices will serve molecular basis for nano-pore devices of which activity is easy to be arbitrarily tuned by light irradiation.

Research Products

• [Journal Article] A Structured Monodisperse PEG for the Effective Suppression of Protein Aggregation (2013)
  • Author(s): Takahiro Muraoka, Kota Adachi, Mihoko Ui, Shunichi Kawasaki, Nabanita Sadhukhan, Hamki Obara, Hidehito Tochio, Masahiro Shirakawa, and Kazushi Kinbara
  • Journal Title: Angew. Chem., Int. Ed Volume: 52 Issue: 9 Pages: 2430-2434
  • DOI: 10.1002/anie.201206563
  • Peer Reviewed
• [Journal Article] Mutations for decreasing the immunogenicity and maintaining the function of core streptavidin. (2012)
  • Author(s): Yumura K
  • Journal Title: Protein Sci. Volume: Feb;22(2) Issue: 2 Pages: 213-221
  • DOI: 10.1002/pro.2203
  • Peer Reviewed
• [Journal Article] Amplification of Light-induced Molecular-Shape Changeby Supramolecular Machines (2012)
  • Author(s): Mihoko Ui, Yoshikazu Tanaka, Kazushi Kinbara
  • Journal Title: Journal of Photopolymer Science and Technology Volume: 25 Pages: 655-658
• [Journal Article] Application of Photoactive Yellow Protein as a Photoresponsive Module for Controlling Hemolytic Activity of Staphylococcal α-Hemolysin (2012)
  • Author(s): M. Ui, et al.
  • Journal Title: Chemical Communications Volume: 48 Issue: 39 Pages: 4737-4739
  • DOI: 10.1039/c2cc18118e
  • Peer Reviewed
• [Journal Article] Amplification of Light-induced Molecular-Shape Change by Supramolecular Machines (2012)
  • Author(s): M. Ui, et al.
  • Journal Title: Journal of Photopolymer Science and Technology Volume: 25 Issue: 5 Pages: 655-658
  • DOI: 10.2494/photopolymer.25.655
  • NAID: 130004833493
  • ISSN: 0914-9244, 1349-6336
• [Journal Article] Application of Photoactive Yellow Protein as a Photoresponsive Module for Controlling Hemolytic Activity of Staphylococcal a-Hemolysin (2012)
  • Author(s): Mihoko Ui, Yoshikazu Tanaka, Yasuyuki Araki, Takehiko Wada, Toshiaki Takei, Kouhei Tsumoto, Sumire Endo, and Kazushi Kinbara
  • Journal Title: Chemical Communications Volume: 48 Pages: 4737-4739
  • Peer Reviewed
• [Presentation] 機能性分子導入による孔形成蛋白質alpha-hemolyinの機能制御 (2013)
  • Author(s): 宇井美穂子,播磨耕祐,田中良和,田端和仁,野地博行,秋山公男,武井俊朗,津本浩平,金原数
  • Organizer: 日本化学会第93回春期年会
  • Place of Presentation: 京都
• [Presentation] cAMPの可逆制御を目指した光応答性PDEの創製 (2013)
  • Author(s): 新井康広,宇井美穂子,村上慎,荒木保幸,和田健彦,高橋泰人,金原数
  • Organizer: 日本化学会第93回春期年会
  • Place of Presentation: 京都
• [Presentation] 機能性分子導入による孔形成蛋白質 alpha-hemolyinの機能制御 (2013)
  • Author(s): 宇井美穂子・播磨耕祐・田中良和・田端和仁 野地博行・武井俊朗・津本浩平・金原数
  • Organizer: 日本化学会第93春季年会(2013)
  • Place of Presentation: 滋賀
• [Presentation] cAMPの可逆制御を目指した光応答性PDEの創製 (2013)
  • Author(s): 新井康広・宇井美穂子・村上慎・荒木保幸・和田健彦・高橋泰人・金原数
  • Organizer: 日本化学会第93春季年会(2013)
  • Place of Presentation: 滋賀
• [Presentation] 機能性人工分子が形作るナノ細孔の光制御 (2012)
  • Author(s): 宇井美穂子,播磨耕祐,田中良和,田端和仁,野地博行,秋山公男,金原数
  • Organizer: 日本生物物理学会東北支部会2012,日本
  • Place of Presentation: 仙台
  • Year and Date: 2012-12-21
• [Presentation] Design of Photoresponsive Phosphodiesterase toward Reversible Control of cAMP (2012)
  • Author(s): Y. Arai, M. Ui, H. Takahashi, K.Kinbara
  • Organizer: 第9回国際高分子会 議(The 9th SPSJ International Polymer Conference(IPC2012))
  • Place of Presentation: 日本 , 神戸
• [Presentation] Development of Engineered Photoactive Yellow Protein as a Photoresponsive module for Controlling Biological Events (2012)
  • Author(s): Yusuke Miyauchi, Mihoko Ui, MakotoMurakami, Yasuyuki Araki, Takehiko Wada, Kazushi Kinbara
  • Organizer: 第9回国際高分子会議(The 9th SPSJ International Polymer Conference(IPC2012))
  • Place of Presentation: 日本, 神戸
• [Presentation] Discrete Non-Linear PEGs for Protein Manipulation (2012)
  • Author(s): T. Muraoka, K. Adachi, M. Ui, S.Kawasaki, N. Sadhukhan, H. Obara, H. Tochio, M. Shirakawa, K. Kinbara
  • Organizer: 第9回国際高分子会議 (The 9th SPSJ International Polymer Conference (IPC2012))
  • Place of Presentation: 日本, 神戸
• [Presentation] 光応答性モジュールを指向した改変型PYPの創製 (2012)
  • Author(s): 宮内佑輔,宇井美穂子,村上慎,荒木保幸,和田健彦,金原数
  • Organizer: 第2回CSJ化学フェスタ2012,日本
  • Place of Presentation: 東京
• [Presentation] Synthesisof PEG Derivatives and Their Application in Protein Stabilization (2012)
  • Author(s): Nabanita Sadhukhan, Takahiro Muraoka, Mihoko Ui, Kazushi Kinbara
  • Organizer: 第61回高分子討論会 (61st Symposium on Macromolecules)
  • Place of Presentation: 日本 , 名古屋
• [Presentation] 光応答性分子を用いた孔形成蛋白質alpha-hemolysinの光制御 (2012)
  • Author(s): 宇井美穂子,田中良和,荒木保幸,和田健彦,武井俊朗,津本浩平,金原数
  • Organizer: 第6回バイオ関連化学合同シンポジウム
  • Place of Presentation: 日本,札幌
• [Presentation] Design of Photo-responsive Phosphodiesterase toward Reversible Control of cAMP (2012)
  • Author(s): Yasuhiro Arai, Mihoko Ui, Hiroto Takahashi, Kazushi Kinbara
  • Organizer: キャンパスアジアサマースクール
  • Place of Presentation: 日本,仙台
• [Presentation] Construction of photocontrollable nano-pore devises by grafting an engineered transmembrane units (2012)
  • Author(s): Mihoko Ui, Kousuke Harima, Yoshikazu Tanaka, Toshiaki Takei, Kouhei Tsumoto, Kazushi Kinbara
  • Organizer: The second Asian Chemical Biology Conference ACBC2012
  • Place of Presentation: 日本,沖縄
• [Presentation] Designer PEG-Conjugates for Protein Stabilization (2012)
  • Author(s): Nabanita Sadhukhan, Takahiro Muraoka, Mihoko Ui, Kazushi Kinbara
  • Organizer: The second Asian Chemical Biology Conference ACBC2012
  • Place of Presentation: 日本,沖縄
• [Presentation] 光応答性分子を用いた孔形成毒素alpha-hemolysinの光制御系の構築 (2012)
  • Author(s): 宇井美穂子,播磨耕祐,田中良和,荒木保幸,和田健彦,武井俊朗,津本浩平,金原数
  • Organizer: 第22回バイオ・高分子シンポジウム
  • Place of Presentation: 日本,東京
• [Presentation] 光受容蛋白質Phtoactive Yellow proteinを用いたStaphylococcal alpha-Hemolysinの溶血活性制御 (2012)
  • Author(s): 宇井美穂子,田中良和,荒木保幸,和田健彦,武井俊朗,津本浩平,金原数
  • Organizer: 第61回高分子学会年次大会(61st SPSJ Annual meeting)
  • Place of Presentation: 日本,横浜
• [Presentation] 光応答性蛋白質Photoactive Yellow protein融合Staphylococcal alpha-Hemolysinの機能制御 (2012)
  • Author(s): 宇井美穂子,田中良和,荒木保幸,和田健彦,武井俊朗,津本浩平,金原数
  • Organizer: 日本化学会第92回春期年会
  • Place of Presentation: 神奈川
• [Presentation] 人工分子を用いたStaphylococcus aureusα-hemolysinの溶血活性の制御 (2012)
  • Author(s): 播磨耕祐,宇井美穂子,田中良和,石井則行,金原数
  • Organizer: 日本化学会第92回春期年会
  • Place of Presentation: 神奈川
• [Presentation] 白川昌宏,金原数,構造化PEG分子の合成とその性質(1)(Synthesis and Characterization of Discrete Non-Linear PEGs(1)) (2012)
  • Author(s): 村岡貴博,安達皓太,宇井美穂子,河崎俊一,杤尾豪人
  • Organizer: 日本化学会第92春季年会(The 92nd Annual Meeting of the Chemical Society of Japan)
  • Place of Presentation: 日本,神奈川
• [Presentation] Self-Assembling Discrete Non-Linear PEGs for Protein Manipulation (2012)
  • Author(s): Takahiro Muraoka, Kota Adachi, Mihoko Ui, Shunichi Kawasaki, Hidehito Tochio, Masahiro Shirakawa, Kazushi Kinbara
  • Organizer: 2012国際コロイド界面会議 (International Association of Colloid and Interface Scientists (IACIS 2012))
  • Place of Presentation: 東京
• [Presentation] Development of Molecular Library for Protein Manipulation (2012)
  • Author(s): Nabanita Sadhukhan, Takahiro Muraoka, Mihoko Ui, Kazushi Kinbara
  • Organizer: 第61回高分子学会年次大会 (61st SPSJ Annual meeting)
  • Place of Presentation: 横浜
• [Presentation] 光受容蛋白質Phtoactive Yellow proteinを用いた Staphylococcal alpha-Hemolysinの溶血活性制御 (2012)
  • Author(s): 宇井美穂子, 田中良和, 荒木保幸, 和田健彦, 武井俊朗, 津本浩平, 金原 数
  • Organizer: 第61回高分子学会年次大会(61st SPSJ Annual meeting )
  • Place of Presentation: 横浜
• [Presentation] Synthesis and Characterization of Discrete PEGs (2012)
  • Author(s): 村岡貴博, 安達皓太, 宇井美穂子, 河崎俊一, 小原春樹, 杤尾豪人, 白川昌宏, 金原数
  • Organizer: 第61回高分子学会年次大会(61st SPSJ Annual meeting )
  • Place of Presentation: 横浜
• [Presentation] 光応答性分子を用いた孔形成毒素alpha-hemolysinの光制御系の構築 (2012)
  • Author(s): 宇井美穂子, 播磨耕祐, 田中良和, 荒木保幸, 和田健彦, 武井俊朗, 津本浩平, 金原数
  • Organizer: 第22回バイオ・高分子シンポジウム
  • Place of Presentation: 東京
• [Presentation] 光応答性分子を用いた孔形成蛋白質alpha-hemolysinの光制御 (2012)
  • Author(s): 宇井美穂子, 田中良和, 荒木保幸, 和田健彦, 武井俊朗, 津本浩平, 金原 数
  • Organizer: 第6回バイオ関連化学合同シンポジウム
  • Place of Presentation: 札幌
• [Presentation] Discrete PEGs with Distinctive Characteristics (2012)
  • Author(s): 村岡貴博, 安達皓太, 宇井美穂子, 河崎俊一, Nabanita SADHUKHAN, 小原春樹, 杤尾豪人, 白川昌宏, 金原数
  • Organizer: 第61回高分子討論会
  • Place of Presentation: 名古屋
• [Presentation] Synthesis of PEG Derivatives and Their Application in Protein Stabilization (2012)
  • Author(s): Nabanita Sadhukhan, Takahiro Muraoka, Mihoko Ui, Kazushi Kinbara
  • Organizer: 第61回高分子討論会
  • Place of Presentation: 名古屋
• [Presentation] 光応答性モジュールを指向した改変型PYPの創製 (2012)
  • Author(s): 宮内佑輔, 宇井美穂子, 村上慎, 荒木保幸, 和田健彦, 金原数
  • Organizer: 第2回CSJ化学フェスタ2012
  • Place of Presentation: 東京
• [Presentation] Construction of photocontrollable nano-pore devises by grafting an engineered transmembrane units (2012)
  • Author(s): Mihoko Ui, Kousuke Harima, Yoshikazu Tanaka, Toshiaki Takei, Kouhei Tsumoto, Kazushi Kinbara
  • Organizer: The second Asian Chemical Biology Conference ACBC2012
  • Place of Presentation: 沖縄
• [Presentation] Designer PEG-Conjugates for Protein Stabilization (2012)
  • Author(s): Nabanita Sadhukhan, Takahiro Muraoka, Mihoko Ui, Kazushi Kinbara
  • Organizer: The second Asian Chemical Biology Conference ACBC2012
  • Place of Presentation: 沖縄
• [Presentation] Design of Photo-responsive Phosphodiesterase toward Reversible Control of cAMP (2012)
  • Author(s): Yasuhiro Arai, Mihoko Ui, Hiroto Takahashi, Kazushi Kinbara
  • Organizer: キャンパスアジアサマースクール
  • Place of Presentation: 仙台
• [Presentation] Design of Photoresponsive Phosphodiesterase toward Reversible Control of cAMP (2012)
  • Author(s): Y. Arai, M. Ui, H. Takahashi, K. Kinbara
  • Organizer: The 9th SPSJ International Polymer Conference(IPC2012)
  • Place of Presentation: 神戸
• [Presentation] Development of Engineered Photoactive Yellow Protein as a Photoresponsive module for Controlling Biological Events (2012)
  • Author(s): Yusuke Miyauchi, Mihoko Ui, Makoto Murakami, Yasuyuki Araki, Takehiko Wada, Kazushi Kinbara
  • Organizer: The 9th SPSJ International Polymer Conference(IPC2012)
  • Place of Presentation: 神戸
• [Presentation] Discrete Non-Linear PEGs for Protein Manipulation (2012)
  • Author(s): T. Muraoka, K. Adachi, M. Ui, S. Kawasaki, N. Sadhukhan, H. Obara, H. Tochio, M. Shirakawa, K. Kinbara
  • Organizer: The 9th SPSJ International Polymer Conference (IPC2012)
  • Place of Presentation: 神戸
• [Presentation] 構造化PEG分子の合成とその性質(1) (2012)
  • Author(s): 村岡貴博、安達皓太、宇井美穂子、河崎俊一、杤尾豪人、白川昌宏、金原数
  • Organizer: 日本化学会第92春季年会(2012)
  • Place of Presentation: 慶応義塾大学
• [Presentation] ファージディスプレイ法を活用した新規超分子不斉光反応系の構築-4 (2012)
  • Author(s): 湊咲絵、宮地亜有実、菅原唯、坂本清志、中木戸誠、宇井美穂子、荒木保幸、西嶋政樹、津本浩平、金原数、井上佳久、和田健彦
  • Organizer: 日本化学会第92春季年会(2012)
  • Place of Presentation: 慶応義塾大学
• [Presentation] プレターゲティング法を用いた薬剤輸送のためのコアストレプトアビジン融合抗体 (2012)
  • Author(s): 湯村恭平、宇井美穂子、岡本未央、土井洋文、杉山暁、浜窪隆雄、児玉龍彦、津本浩平
  • Organizer: 日本化学会第92春季年会(2012)
  • Place of Presentation: 慶応義塾大学
• [Presentation] 人工分子を用いたStaphylococcus aureus α-hemolysinの溶血活性制御 (2012)
  • Author(s): 播磨耕祐、宇井美穂子、田中良和、石井則行、金原数
  • Organizer: 日本化学会第92春季年会(2012)
  • Place of Presentation: 慶応義塾大学
• [Presentation] 光応答性蛋白質Phtoactive Yellow protein融合Staphylococcal alpha-Hemolysinの機能制御 (2012)
  • Author(s): 宇井美穂子、田中良和、荒木保幸、和田健彦、武井俊朗、津本浩平、金原数
  • Organizer: 日本化学会第92春季年会(2012)
  • Place of Presentation: 慶応義塾大学
• [Presentation] Development of a structured PEG molecule for protein manipulation (2011)
  • Author(s): Shunichi Kawasaki, Takahiro Muraoka, Mihoko Ui, Kouta Adachi, Kazushi Kinbara
  • Organizer: グローバルCOEサマースクールシンポジウム (Global COE Summer School Symposium)
  • Place of Presentation: 日本,仙台
• [Presentation] 構造化PEG分子の開発とタンパク質マニピュレーションへの応用 (2011)
  • Author(s): 河崎俊一,村岡貴博,宇井美穂子,安達皓太,金原数
  • Organizer: 平成23年度化学系学協会東北大会
  • Place of Presentation: 日本,仙台
• [Presentation] 構造化PEG分子の開発とタンパク質マニピュレーションへの応用 (2011)
  • Author(s): 河崎俊一,村岡貴博,宇井美穂子,安達皓太,金原数
  • Organizer: 第5回バイオ関連化学シンポジウム
  • Place of Presentation: 日本,つくば
• [Presentation] 構造化PEG分子の開発とタンパク質マニピュレーションへの応用 (2011)
  • Author(s): 河崎俊一、村岡貴博、宇井美穂子、安達皓太、金原数
  • Organizer: 第5回バイオ関連シンポジウム
  • Place of Presentation: つくば
• [Presentation] タンパク質操作のための分子ライブラリー構築 (2011)
  • Author(s): Nabanita Sadhukhan、村岡貴博、宇井美穂子、金原数
  • Organizer: 平成23年度化学系学協会東北大会
  • Place of Presentation: 東北大学
• [Presentation] 構造化PEG分子の開発とタンパク質マニピュレーションへの応用 (2011)
  • Author(s): 河崎俊一、村岡貴博、宇井美穂子、安達皓太、金原数
  • Organizer: 平成23年度化学系学協会東北大会
  • Place of Presentation: 東北大学
• [Presentation] 機能性人工分子が形作るナノ細孔の光制御
  • Author(s): 宇井美穂子, 播磨耕祐, 田中良和, 田端和仁, 野地博行, 秋山公男, 金原数
  • Organizer: 日本生物物理学会 東北支部会2012
  • Place of Presentation: 仙台
• [Remarks]
  • URL: http://www.tagen.tohoku.ac.jp/labo/kinbara/index-j.html