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Construction of a photoregulating system for self-assembly proteins

Research Project

Project/Area Number 23750179
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Chemistry related to living body
Research InstitutionTohoku University

Principal Investigator

UI Mihoko  東北大学, 多元物質科学研究所, 助教 (30549580)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords光応答性蛋白質 / 自己集合蛋白質 / photoactive yellow protein / alpha-hemolysin / 光制御 / 光受容蛋白質 / PYP / 孔形成蛋白質 / 溶血活性制御 / 生体分子デバイス
Research Abstract

We constructed two types of photo-controlled nano-pore devices based on PYP photoreceptor-fused staphylococcal pore-forming toxin alpha-hemolysin. The both PYP-fused devices had the similar photocycle as wild type PYP and inhibited the transmission efficiency of ions through the sheep red blood cells membrane under light irradiation. These PYP-fused devices will serve molecular basis for nano-pore devices of which activity is easy to be arbitrarily tuned by light irradiation.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (53 results)

All 2013 2012 2011 Other

All Journal Article (6 results) (of which Peer Reviewed: 4 results) Presentation (46 results) Remarks (1 results)

  • [Journal Article] A Structured Monodisperse PEG for the Effective Suppression of Protein Aggregation2013

    • Author(s)
      Takahiro Muraoka, Kota Adachi, Mihoko Ui, Shunichi Kawasaki, Nabanita Sadhukhan, Hamki Obara, Hidehito Tochio, Masahiro Shirakawa, and Kazushi Kinbara
    • Journal Title

      Angew. Chem., Int. Ed

      Volume: 52 Issue: 9 Pages: 2430-2434

    • DOI

      10.1002/anie.201206563

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mutations for decreasing the immunogenicity and maintaining the function of core streptavidin.2012

    • Author(s)
      Yumura K
    • Journal Title

      Protein Sci.

      Volume: Feb;22(2) Issue: 2 Pages: 213-221

    • DOI

      10.1002/pro.2203

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Amplification of Light-induced Molecular-Shape Changeby Supramolecular Machines2012

    • Author(s)
      Mihoko Ui, Yoshikazu Tanaka, Kazushi Kinbara
    • Journal Title

      Journal of Photopolymer Science and Technology

      Volume: 25 Pages: 655-658

    • Related Report
      2012 Final Research Report
  • [Journal Article] Application of Photoactive Yellow Protein as a Photoresponsive Module for Controlling Hemolytic Activity of Staphylococcal α-Hemolysin2012

    • Author(s)
      M. Ui, et al.
    • Journal Title

      Chemical Communications

      Volume: 48 Issue: 39 Pages: 4737-4739

    • DOI

      10.1039/c2cc18118e

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Amplification of Light-induced Molecular-Shape Change by Supramolecular Machines2012

    • Author(s)
      M. Ui, et al.
    • Journal Title

      Journal of Photopolymer Science and Technology

      Volume: 25 Issue: 5 Pages: 655-658

    • DOI

      10.2494/photopolymer.25.655

    • NAID

      130004833493

    • ISSN
      0914-9244, 1349-6336
    • Related Report
      2012 Annual Research Report
  • [Journal Article] Application of Photoactive Yellow Protein as a Photoresponsive Module for Controlling Hemolytic Activity of Staphylococcal a-Hemolysin2012

    • Author(s)
      Mihoko Ui, Yoshikazu Tanaka, Yasuyuki Araki, Takehiko Wada, Toshiaki Takei, Kouhei Tsumoto, Sumire Endo, and Kazushi Kinbara
    • Journal Title

      Chemical Communications

      Volume: 48 Pages: 4737-4739

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 機能性分子導入による孔形成蛋白質alpha-hemolyinの機能制御2013

    • Author(s)
      宇井美穂子,播磨耕祐,田中良和,田端和仁,野地博行,秋山公男,武井俊朗,津本浩平,金原数
    • Organizer
      日本化学会第93回春期年会
    • Place of Presentation
      京都
    • Related Report
      2012 Final Research Report
  • [Presentation] cAMPの可逆制御を目指した光応答性PDEの創製2013

    • Author(s)
      新井康広,宇井美穂子,村上慎,荒木保幸,和田健彦,高橋泰人,金原数
    • Organizer
      日本化学会第93回春期年会
    • Place of Presentation
      京都
    • Related Report
      2012 Final Research Report
  • [Presentation] 機能性分子導入による孔形成蛋白質 alpha-hemolyinの機能制御2013

    • Author(s)
      宇井美穂子・播磨耕祐・田中良和・田端和仁 野地博行・武井俊朗・津本浩平・金原数
    • Organizer
      日本化学会第93春季年会(2013)
    • Place of Presentation
      滋賀
    • Related Report
      2012 Annual Research Report
  • [Presentation] cAMPの可逆制御を目指した光応答性PDEの創製2013

    • Author(s)
      新井康広・宇井美穂子・村上慎・荒木保幸・和田健彦・高橋泰人・金原数
    • Organizer
      日本化学会第93春季年会(2013)
    • Place of Presentation
      滋賀
    • Related Report
      2012 Annual Research Report
  • [Presentation] 機能性人工分子が形作るナノ細孔の光制御2012

    • Author(s)
      宇井美穂子,播磨耕祐,田中良和,田端和仁,野地博行,秋山公男,金原数
    • Organizer
      日本生物物理学会東北支部会2012,日本
    • Place of Presentation
      仙台
    • Year and Date
      2012-12-21
    • Related Report
      2012 Final Research Report
  • [Presentation] Design of Photoresponsive Phosphodiesterase toward Reversible Control of cAMP2012

    • Author(s)
      Y. Arai, M. Ui, H. Takahashi, K.Kinbara
    • Organizer
      第9回国際高分子会 議(The 9th SPSJ International Polymer Conference(IPC2012))
    • Place of Presentation
      日本 , 神戸
    • Related Report
      2012 Final Research Report
  • [Presentation] Development of Engineered Photoactive Yellow Protein as a Photoresponsive module for Controlling Biological Events2012

    • Author(s)
      Yusuke Miyauchi, Mihoko Ui, MakotoMurakami, Yasuyuki Araki, Takehiko Wada, Kazushi Kinbara
    • Organizer
      第9回国際高分子会議(The 9th SPSJ International Polymer Conference(IPC2012))
    • Place of Presentation
      日本, 神戸
    • Related Report
      2012 Final Research Report
  • [Presentation] Discrete Non-Linear PEGs for Protein Manipulation2012

    • Author(s)
      T. Muraoka, K. Adachi, M. Ui, S.Kawasaki, N. Sadhukhan, H. Obara, H. Tochio, M. Shirakawa, K. Kinbara
    • Organizer
      第9回国際高分子会議 (The 9th SPSJ International Polymer Conference (IPC2012))
    • Place of Presentation
      日本, 神戸
    • Related Report
      2012 Final Research Report
  • [Presentation] 光応答性モジュールを指向した改変型PYPの創製2012

    • Author(s)
      宮内佑輔,宇井美穂子,村上慎,荒木保幸,和田健彦,金原数
    • Organizer
      第2回CSJ化学フェスタ2012,日本
    • Place of Presentation
      東京
    • Related Report
      2012 Final Research Report
  • [Presentation] Synthesisof PEG Derivatives and Their Application in Protein Stabilization2012

    • Author(s)
      Nabanita Sadhukhan, Takahiro Muraoka, Mihoko Ui, Kazushi Kinbara
    • Organizer
      第61回高分子討論会 (61st Symposium on Macromolecules)
    • Place of Presentation
      日本 , 名古屋
    • Related Report
      2012 Final Research Report
  • [Presentation] 光応答性分子を用いた孔形成蛋白質alpha-hemolysinの光制御2012

    • Author(s)
      宇井美穂子,田中良和,荒木保幸,和田健彦,武井俊朗,津本浩平,金原数
    • Organizer
      第6回バイオ関連化学合同シンポジウム
    • Place of Presentation
      日本,札幌
    • Related Report
      2012 Final Research Report
  • [Presentation] Design of Photo-responsive Phosphodiesterase toward Reversible Control of cAMP2012

    • Author(s)
      Yasuhiro Arai, Mihoko Ui, Hiroto Takahashi, Kazushi Kinbara
    • Organizer
      キャンパスアジアサマースクール
    • Place of Presentation
      日本,仙台
    • Related Report
      2012 Final Research Report
  • [Presentation] Construction of photocontrollable nano-pore devises by grafting an engineered transmembrane units2012

    • Author(s)
      Mihoko Ui, Kousuke Harima, Yoshikazu Tanaka, Toshiaki Takei, Kouhei Tsumoto, Kazushi Kinbara
    • Organizer
      The second Asian Chemical Biology Conference ACBC2012
    • Place of Presentation
      日本,沖縄
    • Related Report
      2012 Final Research Report
  • [Presentation] Designer PEG-Conjugates for Protein Stabilization2012

    • Author(s)
      Nabanita Sadhukhan, Takahiro Muraoka, Mihoko Ui, Kazushi Kinbara
    • Organizer
      The second Asian Chemical Biology Conference ACBC2012
    • Place of Presentation
      日本,沖縄
    • Related Report
      2012 Final Research Report
  • [Presentation] 光応答性分子を用いた孔形成毒素alpha-hemolysinの光制御系の構築2012

    • Author(s)
      宇井美穂子,播磨耕祐,田中良和,荒木保幸,和田健彦,武井俊朗,津本浩平,金原数
    • Organizer
      第22回バイオ・高分子シンポジウム
    • Place of Presentation
      日本,東京
    • Related Report
      2012 Final Research Report
  • [Presentation] 光受容蛋白質Phtoactive Yellow proteinを用いたStaphylococcal alpha-Hemolysinの溶血活性制御2012

    • Author(s)
      宇井美穂子,田中良和,荒木保幸,和田健彦,武井俊朗,津本浩平,金原数
    • Organizer
      第61回高分子学会年次大会(61st SPSJ Annual meeting)
    • Place of Presentation
      日本,横浜
    • Related Report
      2012 Final Research Report
  • [Presentation] 光応答性蛋白質Photoactive Yellow protein融合Staphylococcal alpha-Hemolysinの機能制御2012

    • Author(s)
      宇井美穂子,田中良和,荒木保幸,和田健彦,武井俊朗,津本浩平,金原数
    • Organizer
      日本化学会第92回春期年会
    • Place of Presentation
      神奈川
    • Related Report
      2012 Final Research Report
  • [Presentation] 人工分子を用いたStaphylococcus aureusα-hemolysinの溶血活性の制御2012

    • Author(s)
      播磨耕祐,宇井美穂子,田中良和,石井則行,金原数
    • Organizer
      日本化学会第92回春期年会
    • Place of Presentation
      神奈川
    • Related Report
      2012 Final Research Report
  • [Presentation] 白川昌宏,金原数,構造化PEG分子の合成とその性質(1)(Synthesis and Characterization of Discrete Non-Linear PEGs(1))2012

    • Author(s)
      村岡貴博,安達皓太,宇井美穂子,河崎俊一,杤尾豪人
    • Organizer
      日本化学会第92春季年会(The 92nd Annual Meeting of the Chemical Society of Japan)
    • Place of Presentation
      日本,神奈川
    • Related Report
      2012 Final Research Report
  • [Presentation] Self-Assembling Discrete Non-Linear PEGs for Protein Manipulation2012

    • Author(s)
      Takahiro Muraoka, Kota Adachi, Mihoko Ui, Shunichi Kawasaki, Hidehito Tochio, Masahiro Shirakawa, Kazushi Kinbara
    • Organizer
      2012国際コロイド界面会議 (International Association of Colloid and Interface Scientists (IACIS 2012))
    • Place of Presentation
      東京
    • Related Report
      2012 Annual Research Report
  • [Presentation] Development of Molecular Library for Protein Manipulation2012

    • Author(s)
      Nabanita Sadhukhan, Takahiro Muraoka, Mihoko Ui, Kazushi Kinbara
    • Organizer
      第61回高分子学会年次大会 (61st SPSJ Annual meeting)
    • Place of Presentation
      横浜
    • Related Report
      2012 Annual Research Report
  • [Presentation] 光受容蛋白質Phtoactive Yellow proteinを用いた Staphylococcal alpha-Hemolysinの溶血活性制御2012

    • Author(s)
      宇井美穂子, 田中良和, 荒木保幸, 和田健彦, 武井俊朗, 津本浩平, 金原 数
    • Organizer
      第61回高分子学会年次大会(61st SPSJ Annual meeting )
    • Place of Presentation
      横浜
    • Related Report
      2012 Annual Research Report
  • [Presentation] Synthesis and Characterization of Discrete PEGs2012

    • Author(s)
      村岡貴博, 安達皓太, 宇井美穂子, 河崎俊一, 小原春樹, 杤尾豪人, 白川昌宏, 金原数
    • Organizer
      第61回高分子学会年次大会(61st SPSJ Annual meeting )
    • Place of Presentation
      横浜
    • Related Report
      2012 Annual Research Report
  • [Presentation] 光応答性分子を用いた孔形成毒素alpha-hemolysinの光制御系の構築2012

    • Author(s)
      宇井美穂子, 播磨耕祐, 田中良和, 荒木保幸, 和田健彦, 武井俊朗, 津本浩平, 金原数
    • Organizer
      第22回バイオ・高分子シンポジウム
    • Place of Presentation
      東京
    • Related Report
      2012 Annual Research Report
  • [Presentation] 光応答性分子を用いた孔形成蛋白質alpha-hemolysinの光制御2012

    • Author(s)
      宇井美穂子, 田中良和, 荒木保幸, 和田健彦, 武井俊朗, 津本浩平, 金原 数
    • Organizer
      第6回バイオ関連化学合同シンポジウム
    • Place of Presentation
      札幌
    • Related Report
      2012 Annual Research Report
  • [Presentation] Discrete PEGs with Distinctive Characteristics2012

    • Author(s)
      村岡貴博, 安達皓太, 宇井美穂子, 河崎俊一, Nabanita SADHUKHAN, 小原春樹, 杤尾豪人, 白川昌宏, 金原数
    • Organizer
      第61回高分子討論会
    • Place of Presentation
      名古屋
    • Related Report
      2012 Annual Research Report
  • [Presentation] Synthesis of PEG Derivatives and Their Application in Protein Stabilization2012

    • Author(s)
      Nabanita Sadhukhan, Takahiro Muraoka, Mihoko Ui, Kazushi Kinbara
    • Organizer
      第61回高分子討論会
    • Place of Presentation
      名古屋
    • Related Report
      2012 Annual Research Report
  • [Presentation] 光応答性モジュールを指向した改変型PYPの創製2012

    • Author(s)
      宮内佑輔, 宇井美穂子, 村上慎, 荒木保幸, 和田健彦, 金原数
    • Organizer
      第2回CSJ化学フェスタ2012
    • Place of Presentation
      東京
    • Related Report
      2012 Annual Research Report
  • [Presentation] Construction of photocontrollable nano-pore devises by grafting an engineered transmembrane units2012

    • Author(s)
      Mihoko Ui, Kousuke Harima, Yoshikazu Tanaka, Toshiaki Takei, Kouhei Tsumoto, Kazushi Kinbara
    • Organizer
      The second Asian Chemical Biology Conference ACBC2012
    • Place of Presentation
      沖縄
    • Related Report
      2012 Annual Research Report
  • [Presentation] Designer PEG-Conjugates for Protein Stabilization2012

    • Author(s)
      Nabanita Sadhukhan, Takahiro Muraoka, Mihoko Ui, Kazushi Kinbara
    • Organizer
      The second Asian Chemical Biology Conference ACBC2012
    • Place of Presentation
      沖縄
    • Related Report
      2012 Annual Research Report
  • [Presentation] Design of Photo-responsive Phosphodiesterase toward Reversible Control of cAMP2012

    • Author(s)
      Yasuhiro Arai, Mihoko Ui, Hiroto Takahashi, Kazushi Kinbara
    • Organizer
      キャンパスアジアサマースクール
    • Place of Presentation
      仙台
    • Related Report
      2012 Annual Research Report
  • [Presentation] Design of Photoresponsive Phosphodiesterase toward Reversible Control of cAMP2012

    • Author(s)
      Y. Arai, M. Ui, H. Takahashi, K. Kinbara
    • Organizer
      The 9th SPSJ International Polymer Conference(IPC2012)
    • Place of Presentation
      神戸
    • Related Report
      2012 Annual Research Report
  • [Presentation] Development of Engineered Photoactive Yellow Protein as a Photoresponsive module for Controlling Biological Events2012

    • Author(s)
      Yusuke Miyauchi, Mihoko Ui, Makoto Murakami, Yasuyuki Araki, Takehiko Wada, Kazushi Kinbara
    • Organizer
      The 9th SPSJ International Polymer Conference(IPC2012)
    • Place of Presentation
      神戸
    • Related Report
      2012 Annual Research Report
  • [Presentation] Discrete Non-Linear PEGs for Protein Manipulation2012

    • Author(s)
      T. Muraoka, K. Adachi, M. Ui, S. Kawasaki, N. Sadhukhan, H. Obara, H. Tochio, M. Shirakawa, K. Kinbara
    • Organizer
      The 9th SPSJ International Polymer Conference (IPC2012)
    • Place of Presentation
      神戸
    • Related Report
      2012 Annual Research Report
  • [Presentation] 構造化PEG分子の合成とその性質(1)2012

    • Author(s)
      村岡貴博、安達皓太、宇井美穂子、河崎俊一、杤尾豪人、白川昌宏、金原数
    • Organizer
      日本化学会第92春季年会(2012)
    • Place of Presentation
      慶応義塾大学
    • Related Report
      2011 Research-status Report
  • [Presentation] ファージディスプレイ法を活用した新規超分子不斉光反応系の構築-42012

    • Author(s)
      湊咲絵、宮地亜有実、菅原唯、坂本清志、中木戸誠、宇井美穂子、荒木保幸、西嶋政樹、津本浩平、金原数、井上佳久、和田健彦
    • Organizer
      日本化学会第92春季年会(2012)
    • Place of Presentation
      慶応義塾大学
    • Related Report
      2011 Research-status Report
  • [Presentation] プレターゲティング法を用いた薬剤輸送のためのコアストレプトアビジン融合抗体2012

    • Author(s)
      湯村恭平、宇井美穂子、岡本未央、土井洋文、杉山暁、浜窪隆雄、児玉龍彦、津本浩平
    • Organizer
      日本化学会第92春季年会(2012)
    • Place of Presentation
      慶応義塾大学
    • Related Report
      2011 Research-status Report
  • [Presentation] 人工分子を用いたStaphylococcus aureus α-hemolysinの溶血活性制御2012

    • Author(s)
      播磨耕祐、宇井美穂子、田中良和、石井則行、金原数
    • Organizer
      日本化学会第92春季年会(2012)
    • Place of Presentation
      慶応義塾大学
    • Related Report
      2011 Research-status Report
  • [Presentation] 光応答性蛋白質Phtoactive Yellow protein融合Staphylococcal alpha-Hemolysinの機能制御2012

    • Author(s)
      宇井美穂子、田中良和、荒木保幸、和田健彦、武井俊朗、津本浩平、金原数
    • Organizer
      日本化学会第92春季年会(2012)
    • Place of Presentation
      慶応義塾大学
    • Related Report
      2011 Research-status Report
  • [Presentation] Development of a structured PEG molecule for protein manipulation2011

    • Author(s)
      Shunichi Kawasaki, Takahiro Muraoka, Mihoko Ui, Kouta Adachi, Kazushi Kinbara
    • Organizer
      グローバルCOEサマースクールシンポジウム (Global COE Summer School Symposium)
    • Place of Presentation
      日本,仙台
    • Related Report
      2012 Final Research Report
  • [Presentation] 構造化PEG分子の開発とタンパク質マニピュレーションへの応用2011

    • Author(s)
      河崎俊一,村岡貴博,宇井美穂子,安達皓太,金原数
    • Organizer
      平成23年度化学系学協会東北大会
    • Place of Presentation
      日本,仙台
    • Related Report
      2012 Final Research Report
  • [Presentation] 構造化PEG分子の開発とタンパク質マニピュレーションへの応用2011

    • Author(s)
      河崎俊一,村岡貴博,宇井美穂子,安達皓太,金原数
    • Organizer
      第5回バイオ関連化学シンポジウム
    • Place of Presentation
      日本,つくば
    • Related Report
      2012 Final Research Report
  • [Presentation] 構造化PEG分子の開発とタンパク質マニピュレーションへの応用2011

    • Author(s)
      河崎俊一、村岡貴博、宇井美穂子、安達皓太、金原数
    • Organizer
      第5回バイオ関連シンポジウム
    • Place of Presentation
      つくば
    • Related Report
      2011 Research-status Report
  • [Presentation] タンパク質操作のための分子ライブラリー構築2011

    • Author(s)
      Nabanita Sadhukhan、村岡貴博、宇井美穂子、金原数
    • Organizer
      平成23年度化学系学協会東北大会
    • Place of Presentation
      東北大学
    • Related Report
      2011 Research-status Report
  • [Presentation] 構造化PEG分子の開発とタンパク質マニピュレーションへの応用2011

    • Author(s)
      河崎俊一、村岡貴博、宇井美穂子、安達皓太、金原数
    • Organizer
      平成23年度化学系学協会東北大会
    • Place of Presentation
      東北大学
    • Related Report
      2011 Research-status Report
  • [Presentation] 機能性人工分子が形作るナノ細孔の光制御

    • Author(s)
      宇井美穂子, 播磨耕祐, 田中良和, 田端和仁, 野地博行, 秋山公男, 金原数
    • Organizer
      日本生物物理学会 東北支部会2012
    • Place of Presentation
      仙台
    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.tagen.tohoku.ac.jp/labo/kinbara/index-j.html

    • Related Report
      2012 Final Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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