Fabrication of 3D Cell Structures Utilizing Micro Bio-Digital Fabrication Method

• Project/Area Number: 23760241
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Intelligent mechanics/Mechanical systems
• Research Institution: Tokai University
• Principal Investigator: UMEZU Shinjiro 東海大学, 工学部, 助教 (70373032)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: (K)ナノ・マイクロ加工 / マイクロマシン / 人工臓器 / バイオプリント / PELID / inkjet / cell

Research Abstract

Electrostatic micro drop-injection method has good merits those are high resolution to print and ability to eject highly viscous liquid. We demonstrated that living cells and highly viscous scaffolds can be print utilizing electrostatic micro drop-injection method. When syringe that is filled with liquid that contains living cells and syringe that is filled with liquid that contains scaffolds are set, we can print 3D living cell structures. Floating condition is not good for cells. We developed new experimental set-up to reduce floating time of cells. We fabricate 3D bio-device that has blood vessels.

Research Products

• [Journal Article] マイクロデジタルファブリケーション技術による色素増感型太陽電池のチタニア層のパターン形成 (2013)
  • Author(s): 梅津信二郎
  • Journal Title: コンバーテック Volume: 41 Pages: 93-96
• [Journal Article] Development of the Dye-Sensitized Solar Cell by Micro Digital Fabrication (2013)
  • Author(s): Umezu, S
  • Journal Title: J. of Advanced Science Volume: accepted
  • NAID: 130004837664
  • Peer Reviewed
• [Journal Article] Patterning Collagen for 3D Cell Structures (2013)
  • Author(s): Umezu, S.
  • Journal Title: J. of Advanced Science Volume: accepted
  • NAID: 130005113896
  • Peer Reviewed
• [Journal Article] Patterning Collagen Utilizing PELID Method (2013)
  • Author(s): Umezu, S.
  • Journal Title: Advanced Material Research Volume: accepted
  • Peer Reviewed
• [Journal Article] Fundamental Characteristics of Bioprint on Calcium Alginate Gel (2013)
  • Author(s): Umezu, S.
  • Journal Title: Japanese J. Applied Physics Volume: 52 Issue: 5S1 Pages: 05DB20-05DB20
  • DOI: 10.7567/jjap.52.05db20
  • NAID: 210000142225
  • Peer Reviewed
• [Journal Article] マイクロデジタルファブリケーション技術を利用した色素増感型太陽電池の作製 (2013)
  • Author(s): 梅津信二郎
  • Journal Title: 工業化学 Volume: accepted
  • Peer Reviewed
• [Journal Article] BioCell Print Utilizing PELID (Patternin withElectrostatically-Injected Droplet) (2012)
  • Author(s): Umezu, S
  • Journal Title: J. Artificial Life and Robotics Volume: 4 Pages: 116-119
• [Journal Article] マイクロデジタルファブリケーション技術を利用したバイオテクノロジー・グリーンテクノロジーへの応用 (2012)
  • Author(s): 梅津信二郎
  • Journal Title: 日本機械学会トピックス Volume: 115 Pages: 847-847
• [Journal Article] ロール・ツー・ロールプリンテッドエレクトロニクスにおける基幹技術 (2012)
  • Author(s): 橋本巨,梅津信二郎
  • Journal Title: 精密工学会誌 Volume: 78 Pages: 647-677
  • NAID: 130003369732
• [Journal Article] インクジェット技術の太陽電池・バイオ分野への応用 (2012)
  • Author(s): 梅津信二郎
  • Journal Title: 日本画像学会誌 Volume: 51 Pages: 360-366
  • NAID: 130004800852
• [Journal Article] BioCell Print Utilizing PELID (Patterning with Electrostatically-Injected Droplet) Method (2012)
  • Author(s): Umezu, S.
  • Journal Title: J. Artificial Life and Robotics Volume: Volume 17 Issue 1, Pages: 59-62
  • Peer Reviewed
• [Journal Article] マイクロデジタルファブリケーション技術を利用したバイオテクノロジー・グリーンテクノロジーへの応用 (2012)
  • Author(s): 梅津信二郎
  • Journal Title: 日本機械学会 Volume: 115 Pages: 847-847
  • Peer Reviewed
• [Journal Article] ロール・ツー・ロールプリンテッドエレクトロニクスにおける基幹技術 (2012)
  • Author(s): 梅津信二郎
  • Journal Title: 精密工学会誌 Volume: 78 Pages: 647-647
  • NAID: 130003369732
  • Peer Reviewed
• [Journal Article] Development of the Dye-Sensitized Solar Cell by Micro Digital Fabrication
  • Author(s): Umezu, S., Kawata, S. and Kunugi, Y
  • Journal Title: J. of Advanced Science Volume: (accepted)
  • NAID: 130004837664
• [Journal Article] Patterning Collagen for 3D Cell Structures
  • Author(s): Umezu, S. and Aoki, T
  • Journal Title: J. of Advanced Science Volume: (accepted)
  • NAID: 130005113896
• [Journal Article] Fabrication of Wings that Mimic Micro Spikes on Wing Vein of Dragonfly
  • Author(s): Umezu, S. Tanaka T., Azetsu A., and Hashimo H
  • Journal Title: J. of Advanced Science Volume: (accepted)
  • NAID: 130005121905
• [Journal Article] Flow Visualization of Sheet Counting Machine and Improvement for Counting Thin Sheet Materials
  • Author(s): Umezu, S
  • Journal Title: Advanced Material Research Volume: (accepted)
• [Journal Article] Patterning Collagen Utilizing PELID Method
  • Author(s): Umezu, S
  • Journal Title: Advanced Material Research Volume: (accepted)
• [Journal Article] Dye-Sensitized Solar Cell Utilizing Electrostatic Inkjet
  • Author(s): Umezu, S., Kunugi, Y. and Ohmori, H
  • Journal Title: Japanese J. Applied Physics Volume: (in printing)
  • NAID: 210000142249
• [Journal Article] Fundamental Characteristics of Bioprint on Calcium Alginate Gel
  • Author(s): Umezu, S., Hatta T. and Ohmori, H.
  • Journal Title: Japanese J. Applied Physics Volume: (in printing)
  • NAID: 210000142225
• [Journal Article] マイクロデジタルファブリケーション技術を利用した色素増感型太陽電池の作製
  • Author(s): 梅津信二郎
  • Journal Title: 工業化学 Volume: (accepted)
• [Presentation] Fabrication of 3D Bio Devices Utilizing PELID Method (2012)
  • Author(s): Umezu, S
  • Organizer: Proc. of MJIIT-JUC Joint Symposium 2012
• [Presentation] Fabrication of TiO_2 layer Utilizing PELID (Patterning with Electrostatically-Injected Droplet) Method (2012)
  • Author(s): Ishii T., Umezu S., Kunugi, Y., Ishii, A., Ohmori, H
  • Organizer: Proc. of The 2012 International Conference on Flexible and Printed Electronics
• [Presentation] Bio-structure Utilizing PELID (Patterning with Electrostati-cally-Injected Droplet) Method (2012)
  • Author(s): Hatta T., Umezu S., Ohmori, H
  • Organizer: Proc. of The 2012 International Conference on Flexible and Printed Electronics (ICFPE 2012)
• [Presentation] Patterning Calcium Alginate Utilizing PELID (Patterning with Electrostatically-Injected Droplet) Method (2012)
  • Author(s): Umezu, S., Hatta, T., Ohmori, H
  • Organizer: Proc. of The Third International Conference on nanoManufacturing (nanoMan 2012)
• [Presentation] Fabrication of dye-sensitized solar cell utilizing inkjet method (2012)
  • Author(s): Umezu, S., Kunugi, Y
  • Organizer: Proc. of JSME-IIP/ASME-ISPS Joint Conference on Micromechatronics for Information and Precision Equipment (IIP/ISPS Joint MIPE)
• [Presentation] Fabrication of artifical biomimetic wing of dragonfly and its aerodynamic effect (2012)
  • Author(s): Umezu, S., Hashimoto, H., Ohmori, H
  • Organizer: Proceedings of the 12th euspen International Conference
• [Presentation] Digital Fabrication of 3D Bio Devices Utilizing PELID Method (2012)
  • Author(s): Umezu, S., Hatta, T. Ohmori, H
  • Organizer: Proc. of Digital Fabrication
• [Presentation] Fabrication of 3D Bio Devices Utilizing PELID Method (2012)
  • Author(s): Umezu, S
  • Organizer: MJIIT-JUC Joint Symposium 2012
  • Place of Presentation: MJIIT、マレーシア
• [Presentation] Fabrication of TiO2 layer Utilizing PELID (Patterning with Electrostatically-Injected Droplet) Method (2012)
  • Author(s): Ishii T (Umezu S.)
  • Organizer: The 2012 International Conference on Flexible and Printed Electronics (ICFPE 2012)
  • Place of Presentation: 東京大学
• [Presentation] Print of 3D Bio-structure Utilizing PELID (Patterning with Electrostati-cally-Injected Droplet) Method (2012)
  • Author(s): Hatta T (Umezu S.)
  • Organizer: The 2012 International Conference on Flexible and Printed Electronics (ICFPE 2012)
  • Place of Presentation: 東京大学
• [Presentation] Patterning Calcium Alginate Utilizing PELID (Patterning with Electrostatically-Injected Droplet) Method (2012)
  • Author(s): Umezu, S.,
  • Organizer: The Third International Conference on nanoManufacturing (nanoMan 2012)
  • Place of Presentation: 理化学研究所
• [Presentation] Fabrication of dye-sensitized solar cell utilizing inkjet method (2012)
  • Author(s): Umezu, S.,
  • Organizer: JSME-IIP/ASME-ISPS Joint Conference on Micromechatronics for Information and Precision Equipment (IIP/ISPS Joint MIPE) 2012
  • Place of Presentation: サンタクララ大学、アメリカ
• [Presentation] Biocell Print Utilizing PELID Method (2012)
  • Author(s): Shinjiro Umezu
  • Organizer: International Conf. on AROB 2011
  • Place of Presentation: B-Con Plaza, Beppu, Oita, Japan
• [Presentation] 三次元状生体組織作製のためのコラーゲンの精密パターニングと細胞の生化学特性の把握 (2012)
  • Author(s): 梅津信二郎
  • Organizer: 第一回超異分野学会
  • Place of Presentation: 東京，文京区，御茶ノ水女子大学
• [Presentation] Fabrication of Collagen Gel Fiber Films Utilizing PELID Method (2011)
  • Author(s): 秋山寛郎， 梅津信二郎，橋本 巨
  • Organizer: SAS
  • Place of Presentation: 神奈川，平塚，東海大学
• [Presentation] Electrostatic Inkjet method and its applications for bio-technology and green-technology (2011)
  • Author(s): 梅津信二郎
  • Organizer: 第8回色材IT講座(招待講演)
  • Place of Presentation: 東京，御茶ノ水，日本大学
• [Presentation] ラピッドプロトタイピングによるアルギン酸ナトリウムゲル三次元構造の作製 (2011)
  • Author(s): 秋山寛郎，梅津信二郎，大森整，橋本巨
  • Organizer: 日本機械学会関東支部
  • Place of Presentation: 茨城，茨城大学
• [Presentation] 三次元状細胞組織作製のためのPELID法によるコラーゲンのパターニング (2011)
  • Author(s): 野内瑛二郎，磯部優一，梅津信二郎，秋山寛郎，丸山篤史，大沼清
  • Organizer: 日本機械学会関東支部
  • Place of Presentation: 茨城，茨城大学
• [Book] 分散・塗布・乾燥（2.3.6 インクジェット塗布 担当） (2013)
  • Author(s): 梅津信二郎
  • Publisher: テクノシステム
• [Patent(Industrial Property Rights)] 中空部を有する三次元構造体およびその製造方法 (2013)
  • Inventor(s): 梅津信二郎,八田達
  • Industrial Property Rights Holder: 東海大学
  • Filing Date: 2013-02-07
• [Patent(Industrial Property Rights)] 中空部を有する三次元構造体およびその製造方法 (2013)
  • Inventor(s): 梅津信二郎
  • Industrial Property Rights Holder: 梅津信二郎
  • Industrial Property Rights Type: 特許
  • Filing Date: 2013-02-07