Development of highly sensitive bio-immunoassay sensor using Brownian relaxation of the magnetic nanoparticle

• Project/Area Number: 23760369
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Measurement engineering
• Research Institution: Kyushu University
• Principal Investigator: YOSHIDA Takashi 九州大学, システム情報科学研究院, 助教 (30380588)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 計測システム / バイオセンサ / 磁気ナノ粒子 / ブラウン緩和 / ニール緩和 / 磁気モーメント / 磁化 / 粒径分布

Research Abstract

Immunoassay is a test that uses the binding of antibodies to antigens to identify and measure certain disease related protein or pathogenic bacteria. In this study, we developed a rapid and highly sensitive bio-immunoassay sensor system using Brownian relaxation of the magnetic nanoparticles. We could detect 10-6biotins and achieved a detection sensitivity of 20 amol/ml.

Research Products

• [Journal Article] Evaluation of harmonic signals for the detction of magnetic nanoparticles (2012)
  • Author(s): T. Yoshida et al.
  • Journal Title: IEEE TRANSACTIONS ON MAGNETICS Volume: 48 Issue: 11 Pages: 3788-3791
  • DOI: 10.1109/tmag.2012.2198198
  • Peer Reviewed
• [Journal Article] Magnetic fluid dynamics in a rotating magnetic field (2012)
  • Author(s): T. Yoshida, K. Enpuku, J. Dieckhoff, M. Schilling, and F. Ludwig
  • Journal Title: Journal of Applied Physics Volume: Vol.111 Issue: 5 Pages: 0539011-10
  • DOI: 10.1063/1.3688254
  • Peer Reviewed
• [Journal Article] Characterization of Magnetic Markers and Sensors for Liquid-Phase Immunoassays using Brownian Relaxation (2012)
  • Author(s): A.K.BHUIYA
  • Journal Title: IEEE Transactions on Magnetics Volume: 48 Issue: 11 Pages: 2838-2841
  • DOI: 10.1109/tmag.2012.2194137
  • Peer Reviewed
• [Journal Article] Optimization of Magnetic Nanoparticles for Magnetic Particle Imaging (2012)
  • Author(s): F.LUDWIG
  • Journal Title: IEEE Transactions on Magnetics Volume: 48 Issue: 11 Pages: 3780-3783
  • DOI: 10.1109/tmag.2012.2197601
  • Peer Reviewed
• [Journal Article] Homogeneous Bioassays Based on the Manipulation of Magnetic Nanoparticles by Rotating and Alternating Magnetic Fields - a Comparison (2012)
  • Author(s): J.DIECKHOFF
  • Journal Title: IEEE Transactions on Magnetics Volume: 48 Issue: 11 Pages: 3792-3795
  • DOI: 10.1109/tmag.2012.2198797
  • Peer Reviewed
• [Journal Article] Characterization of Resovist Nanoparticles for Magnetic Particle Imaging (2012)
  • Author(s): T. Yoshida, K. Enpuku, F. Ludwig, J. Dieckhoff, T. Wawrzik, A. Lak, and M. Schilling
  • Journal Title: Springer Proceedings in Physics Volume: 140 Pages: 1-5
• [Journal Article] Nonlinear Behavior of Magnetic Fluid in Brownian Relaxation: Numerical Simulation and Derivation of Empirical Model (2012)
  • Author(s): T. Yoshida and K. Enpuku
  • Journal Title: Springer Proceedings in Physics Volume: 140 Pages: 7-11
• [Journal Article] Detection of magnetic nanoparticles using the second-harmonic signal (2011)
  • Author(s): T.Yoshida, K.Enpuku, et al
  • Journal Title: IEEE Trans.Magn. Volume: 47 Issue: 10 Pages: 2863-2866
  • DOI: 10.1109/tmag.2011.2157805
  • Peer Reviewed
• [Journal Article] Liquid-phase immunoassays using Brownian relaxation of magnetic markers (2011)
  • Author(s): AK.Bhuiya, K.Enpuku, et al
  • Journal Title: IEEE Trans.Magn. Volume: 47 Issue: 10 Pages: 2867-2870
  • DOI: 10.1109/tmag.2011.2153828
  • NAID: 10031155260
  • Peer Reviewed
• [Presentation] ブラウン緩和を用いた液相でのバイオ免疫検査法の開発 (2013)
  • Author(s): 平田 智一、吉田 敬、圓福 敬二
  • Organizer: 電気学会マグネティックス研究
  • Place of Presentation: 東京
  • Year and Date: 2013-02-28
• [Presentation] ブラウン緩和を用いた液相でのバイオ免疫検査法の開発 (2013)
  • Author(s): 吉田敬
  • Organizer: 電気学会マグネティックス研究会
  • Place of Presentation: 電力中央研究所（東京都）
• [Presentation] Nonlinear Behavior of Magnetic Fluid in Brownian Relaxation: Numerical Simulation and Derivation ofEmpirical Model (2012)
  • Author(s): T. Yoshida, K. Enpuku
  • Organizer: International Workshop on Magnetic Particle Imaging
  • Place of Presentation: Lubeck, Germany
  • Year and Date: 2012-03-15
• [Presentation] Nonlinear Behavior of Magnetic Fluid in Brownian Relaxation: Numerical Simulation and Derivation of Empirical Model (2012)
  • Author(s): T. Yoshida and K. Enpuku
  • Organizer: International Workshop on Magnetic Particle Imaging
  • Place of Presentation: University of Lubeck (Germany)
• [Presentation] Characterization of Resovist Nanoparticles for Magnetic Particle Imaging (2012)
  • Author(s): T. Yoshida, K. Enpuku, F. Ludwig, J. Dieckhoff, T. Wawrzik, A. Lak, and M. Schilling
  • Organizer: International Workshop on Magnetic Particle Imaging
  • Place of Presentation: University of Lubeck (Germany)
• [Presentation] 磁性ビーズによる医用イメージングとバイオセンシング (2011)
  • Author(s): 吉田敬・円福敬二
  • Organizer: 日本磁気学会ナノバイオ磁気工学専門研究会
  • Place of Presentation: 東京工業大学・田町キャンパス（東京都）