A novel method for comprehensive detection of protein complexes in photosynthetic organisms

• Project/Area Number: 23770035
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Hokkaido University
• Principal Investigator: TAKABAYASHI Atsushi 北海道大学, 低温科学研究所, 助教 (90546417)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: タンパク質複合体解析 / 光合成生物 / データベース / タンパク質複合体 / 光合成 / プロテオーム

Research Abstract

To explore protein complexes systematically, we have tried to identify protein complexes by using the Blue-Native PAGE coupled with LC-MS/MS approach. We applied the approach into several photosynthetic organisms. Then, we constructed a new Web-based database PCoM-DB (http://pcomdb.lowtem.hokudai.ac.jp/proteins/top/) to make our data to public. Users can predict interaction partners with their proteins of interest through a user-friendly interface. I hope that the database to help users to find new protein complexes, which can contributes to understand cellular mechanisms in photosynthetic organisms.

Research Products

• [Journal Article] Functional analysis of light-harvesting-like protein 3 (LIL3) and its light-harvesting chlorophyll-binding motif in Arabidopsis (2014)
  • Author(s): Takahashi K, Takabayashi A, Tanaka A, and Tanaka R
  • Journal Title: The Journal of Biological Chemistry Volume: 289 Pages: 987-999
  • NAID: 120005418077
  • Peer Reviewed
• [Journal Article] Functional Analysis of Light-harvesting-like Protein 3(LIL3) and Its Light-harvesting Chlorophyll-binding Motif in Arabidopsis (2014)
  • Author(s): Kaori Takahashi, Atsushi Takabayashi, Ayumi Tanaka, Ryouichi Tanaka
  • Journal Title: The Journal of Biological Chemistry Volume: 289 Issue: 2 Pages: 987-999
  • DOI: 10.1074/jbc.m113.525428
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Evolutionary changes in chlorophyllide a oxygenase (CAO) structure contribute to the acquisition of a new light-harvesting complex in Micromonas (2013)
  • Author(s): Kunugi M, Takabayashi A, and Tanaka A
  • Journal Title: The Journal of Biological Chemistry Volume: 288 Pages: 19330-19341
  • NAID: 120005326743
  • Peer Reviewed
• [Journal Article] Protein co-migration database (PCoM -DB) for Arabidopsis thylakoids and Synechocystis cells (2013)
  • Author(s): Takabayashi A, Kadoya R, Kuwano M, Kurihara K, Ito H, Tanaka R, Tanaka A
  • Journal Title: SpringerPlus Volume: 2 Pages: 148-148
  • Peer Reviewed
• [Journal Article] Evolutionary changes in chlorophyllide a oxygenase (CAO) structure contribute to the acquisition of a new light-harvesting complex in Micromonas (2013)
  • Author(s): Motoshi Kunugi, Atsushi Takabayashi, and Ayumi Tanaka
  • Journal Title: J. Biol. Chem. Volume: 288 Issue: 27 Pages: 19330-19341
  • DOI: 10.1074/jbc.m113.462663
  • NAID: 120005326743
  • Peer Reviewed
• [Journal Article] Protein co-migration database (PCoM -DB) for Arabidopsis thylakoids and Synechocystis cells (2013)
  • Author(s): Atsushi Takabayashi, Ryosuke Kadoya, Masayoshi Kuwano, Katsunori Kurihara, Hisashi Ito, Ryouichi Tanaka, Ayumi Tanaka
  • Journal Title: SpringerPlus Volume: 2(148) Issue: 1 Pages: 1-10
  • DOI: 10.1186/2193-1801-2-148
  • Peer Reviewed
• [Journal Article] The oligomeric states of photosystems and light-harvesting complexes in the chlorophyll b-less mutant (2011)
  • Author(s): Takabayashi A, Kuihara K, Kuwano M, Kasahara Y, Tanaka R, and Tanaka A
  • Journal Title: Plant & Cell Physiology Volume: 52 Pages: 2103-2114
  • Peer Reviewed
• [Journal Article] Identification of the 7-hydroxymethyl chlorophyll a reductase of the chlorophyll cycle in Arabidopsis (2011)
  • Author(s): Megoro M, Ito H, Takabayashi A, Tanaka R, and Tanaka A
  • Journal Title: Plant Cell Volume: 23 Pages: 3442-3453
  • Peer Reviewed
• [Journal Article] Allocation of absorbed light energy in PSII to thermal dissipations in the presence or absence of PsbS subunits of Rice (2011)
  • Author(s): Ishida, S, Morita K, Kishine M, Takabayashi A, Murakami R, Takeda S, Shimamoto K, Sato F, and Endo T
  • Journal Title: Plant & Cell Physiology Volume: 52 Pages: 1822-1831
  • NAID: 10030304785
  • Peer Reviewed
• [Journal Article] The Oligomeric States of Photosystems and Light-harvesting Complexes in the Chlorophyll b-less Mutant. (2011)
  • Author(s): Takabayashi, A., Kurihara, K., Kuwano, M., Kasahara, Y., Tanaka, R. and Tanaka, A.
  • Journal Title: Plant Cell Physiol Volume: 52(12) Issue: 12 Pages: 2103-2114
  • DOI: 10.1093/pcp/pcr138
  • Peer Reviewed
• [Journal Article] Identification of the 7-Hydroxymethyl Chlorophyll a Reductase of the Chlorophyll Cycle in Arabidopsis. (2011)
  • Author(s): Meguro, M., Ito, H., Takabayashi, A., Tanaka, R. and Tanaka, A.
  • Journal Title: Plant Cell Volume: 23(9) Issue: 9 Pages: 3442-3453
  • DOI: 10.1105/tpc.111.089714
  • Peer Reviewed
• [Journal Article] Allocation of absorbed light energy in Photosystem II to thermal dissipations in the presence or absence of PsbS subunits of rice (2011)
  • Author(s): Ishda, 他8名
  • Journal Title: Plant and Cell Physiology Volume: 52 Issue: 10 Pages: 1822-1831
  • DOI: 10.1093/pcp/pcr119
  • Peer Reviewed
• [Presentation] 新規手法による葉緑体タンパク質複合体の網羅的解析 (2012)
  • Author(s): 高林厚史、栗原克宜、田中亮一、田中歩
  • Organizer: 植物生理学会
  • Place of Presentation: 京都産業大学
  • Year and Date: 2012-03-18
• [Presentation] 新規手法による葉緑体タンパク質複合体の網羅的解析 (2012)
  • Author(s): 高林厚史、栗原克宜、田中亮一、田中歩
  • Organizer: 日本植物生理学会
  • Place of Presentation: 京都産業大学（京都市）
• [Presentation] The application of BN-PAGE for comprehensive detection of the protein complexes in the chloroplast,シンポジウム「Basis, application and future of the blue-native PAGE in plant sciences」 (2011)
  • Author(s): Atsushi Takabayashi
  • Organizer: 植物生理学会
  • Place of Presentation: 東北大学
  • Year and Date: 2011-03-20
• [Remarks] PCoM-DB
  • URL: http://pcomdb.lowtem.hokudai.ac.jp/proteins/top
• [Remarks] PCoM-DB
  • URL: http://pcomdb.lowtem.hokudai.ac.jp/proteins/top
• [Remarks] 生物適応研究室Webページ
  • URL: http://www.lowtem.hokudai.ac.jp/plantadapt/
• [Remarks] PCoM-DB
  • URL: http://pcomdb.lowtem.hokudai.ac.jp/proteins/top