A novel method for comprehensive detection of protein complexes in photosynthetic organisms
| Project/Area Number |
23770035
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | タンパク質複合体解析 / 光合成生物 / データベース / タンパク質複合体 / 光合成 / プロテオーム |
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| Research Abstract |
To explore protein complexes systematically, we have tried to identify protein complexes by using the Blue-Native PAGE coupled with LC-MS/MS approach. We applied the approach into several photosynthetic organisms. Then, we constructed a new Web-based database PCoM-DB (http://pcomdb.lowtem.hokudai.ac.jp/proteins/top/) to make our data to public. Users can predict interaction partners with their proteins of interest through a user-friendly interface. I hope that the database to help users to find new protein complexes, which can contributes to understand cellular mechanisms in photosynthetic organisms.
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Report
(4 results)
Research Products
(19 results)
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[Journal Article] The Oligomeric States of Photosystems and Light-harvesting Complexes in the Chlorophyll b-less Mutant.2011
Author(s)
Takabayashi, A., Kurihara, K., Kuwano, M., Kasahara, Y., Tanaka, R. and Tanaka, A.
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Journal Title
Plant Cell Physiol
Volume: 52(12)
Issue: 12
Pages: 2103-2114
DOI
Related Report
Peer Reviewed
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