Development of new method in structural analysis for larger proteinsusing nuclear magnetic resonances

• Project/Area Number: 23770111
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Structural biochemistry
• Research Institution: Nagoya University
• Principal Investigator: MIYANOIRI Yohei 名古屋大学, 理学研究科, 特任助教 (80547521)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: NMR / 蛋白質 / 核磁気共鳴法 / 高分子量蛋白質 / SAIL / SAIL法

Research Abstract

We have developed new SAIL aromatic amino acids which are optimized relaxation properties in aromatic ring and successfully observed extremely well-separated aromatic CH cross peaks in TROSY experiment, even for an 82kDa E. colimalate synthase G (MSG) protein. Using our new SAILaromatic CH TROSY method, we could clearly assign many NOE signals among aromatic CH, methyl and amide signals which are very important for determining the precise structure of large molecular proteins.

Research Products

• [Journal Article] Molecular bases of multimodal regulation of a fungal transient receptor potential (TRP) channel (2013)
  • Author(s): Ihara M,Hamamoto S, Miyanoiri Y, Takeda M, Kainosho M, Yabe I, Uozumi N, Yamashita A
  • Journal Title: Journal of Biological Chemistry Volume: (in press) Issue: 21 Pages: 15303-17
  • DOI: 10.1074/jbc.m112.434795
  • Peer Reviewed
• [Journal Article] Molecular bases of multimodal regulation of a fungal transient receptor potential (TRP) channel (2013)
  • Author(s): Ihara Makoto
  • Journal Title: Journal of Biological Chemistry Volume: in press
  • Peer Reviewed
• [Journal Article] Stereo-array isotope labeling method for studying protein structure and dynamics (2012)
  • Author(s): 宮ノ入 洋平
  • Journal Title: Advances in Experimental Medicine and Biology Volume: 992 Pages: 83-93
  • DOI: 10.1007/978-94-007-4954-2_5
  • ISBN: 9789400749535, 9789400749542
• [Journal Article] Alternative SAIL-Trp for robust aromatic signal assignment and determination of the X(2) conformation by intra-residue NOEs (2011)
  • Author(s): Y.Miyanoiri, M.Takeda, J.-G.Jee, A.M.Ono, K.Okuma, T.Terauchi, M.Kainosho
  • Journal Title: J.Biomol.NMR Volume: 51 Issue: 4 Pages: 425-435
  • DOI: 10.1007/s10858-011-9568-3
  • Peer Reviewed
• [Presentation] SAIL 法による巨大蛋白質の新規立体構造解析法の開発 (2012)
  • Author(s): 宮ノ入洋平, 武田光広, 大熊宏昌, 小野明, 寺内勉, 甲斐荘正恒
  • Organizer: 第51回 NMR 討論会
  • Place of Presentation: ウインクあいち(愛知県名古屋市)
• [Presentation] SAIL aromatic CH TROSY to investigate larger proteins (2012)
  • Author(s): Miyanoiri Y, Takeda M, Ono AM, Okuma K, Terauchi T, Kainosho M
  • Organizer: International Conference on Magnetic Resonance in Biological Systems LYON 2012
  • Place of Presentation: Lyon Convention Centre(Lyon, France)
• [Presentation] SAIL aromatic CH TROSY to investigate larger proteins (2012)
  • Author(s): Miyanoiri Yohei
  • Organizer: International Conference on Magnetic Resonance in Biological Systems LYON 2012
  • Place of Presentation: Lyon Convention Centre (Lyon, France)
• [Presentation] SAIL法による巨大蛋白質の新規立体構造解析法の開発 (2012)
  • Author(s): 宮ノ入洋平
  • Organizer: 第５１回NMR討論会
  • Place of Presentation: ウインクあいち(愛知県)
• [Presentation] SAIL aromatic CH TROSY to investigate larger proteins and protein complexes (2011)
  • Author(s): Miyanoiri Y, Takeda M, Jee J, Ono AM, Okuma K, Terauchi T, Kainosho M
  • Organizer: The international Symposium on Nuclear Magnetic Resonance 2011
  • Place of Presentation: 大さん橋ホール(神奈川県横浜市)
• [Presentation] Various applications of SAIL aromatic amino acids for NMR studies of proteins (2011)
  • Author(s): Miyanoiri Y, Takeda M, Jee J, Ono AM, Okuma K, Terauchi T, Kainosho M
  • Organizer: The 4thAsia-Pacific NMR Symposium
  • Place of Presentation: Central Garden Beijing Hotel (Beijing, China)
• [Presentation] Various applications of SAIL aromatic amino acids for NMR studies of proteins (2011)
  • Author(s): Yohei Miyanoiri, Mitsuhiro Takeda, JunGoo Jee, Akira Mei Ono, Kosuke Okuma, Tsutomu Terauchi, Masatsune Kainosho
  • Organizer: The 4th Asia-Pacific NMR Symposium
  • Place of Presentation: Beijing, China
• [Presentation] SAIL aromatic CH TROSY to investigate larger proteins and protein complexes (2011)
  • Author(s): Yohei Miyanoiri, Mitsuhiro Takeda, JunGoo Jee, Akira Mei Ono, Kosuke Okuma, Tsutomu Terauchi, Masatsune Kainosho
  • Organizer: The International Symposium on Nuclear Magnetic Resonance 2011
  • Place of Presentation: Yokohama, Japan
• [Remarks]
  • URL: http://str.bio.nagoya-u.ac.jp:8080/Plone/753265908358
• [Remarks] 名古屋大学大学院理学研究科附属構造生物学研究センター 甲斐荘グループ
  • URL: http://str.bio.nagoya-u.ac.jp:8080/Plone/753265908358