In vitro reconstitution of endosomal/lysosomal membrane fusion machinery with purified proteins and defined lipids
| Project/Area Number |
23770117
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Structural biochemistry
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| Research Institution | Osaka University |
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Principal Investigator |
MIMA Joji 大阪大学, たんぱく質研究所, 准教授 (30335301)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
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| Keywords | 細胞小器官 / 細胞内膜交通 / 細胞内膜融合 / プロテオリポソーム / 試験管内再構成 / SNAREタンパク質 / 蛋白質 / 脂質 |
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| Research Abstract |
Here, we reported the fusion specificity of reconstituted proteoliposomes bearing purified SNARE proteins in yeast vacuoles and other organelles,to study whether and how SNAREs mediate the compartmental specificity of intracellular membrane fusion. We found that not only vacuolar R-SNARE, but also the non-cognate endosomal and ER-Golgi R-SNAREs caused efficient fusion with vacuolar Qabc-SNAREs. In contrast, their fusion is blocked completely by replacing the vacuolar Qc-SNARE with the non-cognate endosomal counterparts. Our current study establishes that an appropriate assembly of 3Q-SNAREs is crucial for mediating fusion specificity, whereas R-SNARE itself has little contribution to fusion specificity.
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Report
(3 results)
Research Products
(8 results)
