Study of human HLTF, a ubiquitin ligase for PCNA under DNA damage
Project/Area Number |
23770125
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Structural biochemistry
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Research Institution | Yokohama City University |
Principal Investigator |
HISHIKI Asami 横浜市立大学, 大学院・生命ナノシステム科学研究科, 特任助教 (60571172)
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Project Period (FY) |
2011 – 2012
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Project Status |
Completed (Fiscal Year 2012)
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Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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Keywords | X線結晶解析 / 国際情報交流 |
Research Abstract |
Helicase-Like Transcription Factor (HLTF) is involved in template switching pathway, one of DNA damage tolerance mechanism, which allows continuous DNA synthesis even in the presence of DNA lesion. HLTF consists of RING, Snf2, and HIRAN domains. RING and Snf2 domains are responsible for ubiquitination of PCNA and unwinding of DNA, respectively. The N-terminal HIRAN domain is predicted as a novel DNA-binding domain. We tried to prepare human HLTF (hHLTF) RING and HIRAN domains as recombinant proteins. We analyzed interaction between hHLTF HIRAN domain and DNA by Electrophoresis Mobility Shift Assay.
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Report
(3 results)
Research Products
(9 results)
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[Journal Article] Identification of a small molecule PCNA inhibitor that disrupts interactions with PIP-Box proteins and inhibits DNA replication2012
Author(s)
Chandanamali Punchihewa, Akira Inoue, Asami Hishiki, Yoshihiro Fujikawa, Michele Connelly, Benjamin Evison, Youming Shao, Richard Heath, Isao Kuraoka, Patrick Rodrigues, Hiroshi Hashimoto, Masanobu Kawanishi, Mamoru Sato, Takashi Yagi, Naoaki Fujii
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Journal Title
J Biol Chem
Volume: 287
Issue: 17
Pages: 14289-14300
DOI
Related Report
Peer Reviewed
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[Presentation] En bloc transfer of poly-ubiquitin chains to PCNA in vitro is mediated by two different human E2-E3 pairs.2012
Author(s)
Masuda, Y., Suzuki, M., Kawai, H., Hishiki, A., Hashimoto, H., Masutani, C., Hishida, T., Suzuki, F., Kamiya, K.
Organizer
The 8th 3R Symposium
Place of Presentation
Awaji Yumebutai International Conference Center
Related Report
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