Metal enhanced effect for stable single molecule imaging

• Project/Area Number: 23770168
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biophysics
• Research Institution: Osaka University (2012-2013); Hokkaido University (2011)
• Principal Investigator: ARAI Yoshiyuki 大阪大学, 産業科学研究所, 助教 (20444515)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: １分子 / 金属増強効果 / 金属ナノ粒子 / １分子蛍光観察 / 1分子蛍光観察 / 金属増強 / 蛍光タンパク質 / ImageJ / １分子観察

Research Abstract

Single molecule imaging is a powerful technique for the direct observation of dynamics of biomolecules. However, photo-bleaching and signal fluctuations of fluorophores prevent long time observation. To overcome this problem, here, we made stable single molecule observation system by using nano-particle metal enhancement effect for fluorescent probes. We could observe signal enhancement of single molecule fluorescence by Ag nano-particles with Au as core. In addition, by coating SiO2 on Ag nano-particles coated glass surface, we could maintain the mammalian cell state and could observe metal enhanced fluorescent signals of fluorescently labeled epidermal growth factor.

Research Products

• [Journal Article] Statistical Characterisation of Single-stranded DNA Motion near Glass Surface beyond Diffusion Coefficient (2014)
  • Author(s): Satoshi UEHARA, Itsuo HANASAKI, Yoshiyuki ARAI, Takeharu NAGAI, and Satoyuki KAWANO
  • Journal Title: Micro & Nano Letters Volume: E-First articles Issue: 4 Pages: 1-4
  • DOI: 10.1049/mnl.2013.0668
  • Peer Reviewed
• [Journal Article] Rapid severing andmotility of cp-actin filaments arerequired for the chloroplast avoidanceresponse (2013)
  • Author(s): Kong, S.-G., Y. Arai, N. Suetsugu, T.Yanagida, M. Wada
  • Journal Title: Plant Cell Volume: 25 Issue: 2 Pages: 572-590
  • DOI: 10.1105/tpc.113.109694
  • Peer Reviewed
• [Journal Article] Rab6a releases LIS1 from a dynein idling complex and activates dynein for retrograde movement. (2013)
  • Author(s): Yamada M, Kumamoto K, Mikuni S, Arai Y, Kinjo M, Nagai T, Tsukasaki Y, Watanabe TM, Fukui M, Jin M, Toba S, Hirotsune S.
  • Journal Title: Nat Commun. Volume: 4:2033 Issue: 1 Pages: 2033-2033
  • DOI: 10.1038/ncomms3033
  • Peer Reviewed
• [Journal Article] Importin alpha subtypes determine differential transcription factor localization in embryonic stem cells maintenance (2013)
  • Author(s): Yasuhara N, Yamagishi R, Arai Y, Mehmood R, Kimoto C, Fujita T, Touma K, Kaneko A, Kamikawa Y, Moriyama T, Yanagida T, Kaneko H, Yoneda Y.
  • Journal Title: Dev. Cell Volume: 26 Issue: 2 Pages: 123-135
  • DOI: 10.1016/j.devcel.2013.06.022
  • Peer Reviewed
• [Journal Article] Extensive use of FRET in biological imaging (2013)
  • Author(s): Arai Y, Nagai T.
  • Journal Title: Microscopy Volume: 62 Issue: 4 Pages: 1-3
  • DOI: 10.1093/jmicro/dfs114
  • Peer Reviewed
• [Journal Article] Video-rate imaging of luminescent tumour cells in freely moving unshaved mice. (2013)
  • Author(s): Saito K, Higuchi Y, Arai Y, and Nagai T.
  • Journal Title: Protocol Exchange Volume: --
  • DOI: 10.1038/protex.2013.024
• [Journal Article] Simultaneous Observation of the Lever Arm and Head Explains Myosin VI Dual Function. (2012)
  • Author(s): Keigo Ikezaki et al.
  • Journal Title: Small Volume: 8 Issue: 19 Pages: 3035-3040
  • DOI: 10.1002/smll.201200765
  • Peer Reviewed
• [Journal Article] Optogenetic activation during "dead-time" of detector enables well compatible fluorescence imaging with increasing time resolution and reducing background noise (2012)
  • Author(s): Chang YF, Arai Y, Nagai T
  • Journal Title: Neuroscience Research Volume: 73 Issue: 4 Pages: 341-347
  • DOI: 10.1016/j.neures.2012.05.007
  • Peer Reviewed
• [Journal Article] Luminescent proteins for high-speed single-cell and whole-body imaging (2012)
  • Author(s): Saito K, Chang YF, Horikawa K, Hatsugai N, Higuchi Y, Hashida M, Yoshida Y, Matsuda T, Arai Y, Nagai T
  • Journal Title: Nature communication Volume: 3 Issue: 1 Pages: 1262-1262
  • DOI: 10.1038/ncomms2248
  • Peer Reviewed
• [Journal Article] Conjugation of Both On-axis and Off-axis Light in Nipkow Disk Confocal Microscope to Increase Availability of Incoherent Light Source (2011)
  • Author(s): Saito K, Arai Y, Zhang J, Kobayashi K, Tani T, Nagai T.
  • Journal Title: Cell Structure and Function Volume: 36 Issue: 2 Pages: 237-246
  • DOI: 10.1247/csf.11026
  • NAID: 130004137572
  • ISSN: 0386-7196, 1347-3700
  • Peer Reviewed
• [Presentation] Lossless fluorescence and chemiluminescence imaging with optogenetic activation at real time (2013)
  • Author(s): Y. Arai, Y.-F. Chang and T. Nagai
  • Organizer: The 16th SANKEN International 2013, The 11th SANKEN Nanotechnology Symposium
  • Place of Presentation: 大阪大学産業科学研究所
• [Presentation] Fast single molecule particle tracking and analysis plugin with Java Native Interface (2012)
  • Author(s): Arai Y.
  • Organizer: ImageJ User and Developer Conference 2012
  • Place of Presentation: Center de Recherche Public Henri Tudor, Luxembourg
• [Presentation] Fast single molecule particle tracking and analysis plugin with Java Native Interface (2012)
  • Author(s): Arai Y
  • Organizer: ImageJ User and Developer Conference 2012
  • Place of Presentation: Mondorf-les-Bains, Luxemburg
• [Remarks] 1分子輝点追跡ソフトウェアParticle Track and Analysis
  • URL: http://www.sanken.osaka-u.ac.jp/labs/bse/ImageJcontents/frameImageJ.html
• [Remarks] 新学術領域「少数性生物学」第1回トレーニングコース 講師,大阪大学産業科学研究所, 2013年7月28日-8月10日
• [Remarks] 新井由之,輝点自動検出・追跡ツールの開発,「画像科学」夏の勉強会,核融合科学研究所(岐阜), 2012年9月25日-28日(招待講演)