| Project/Area Number |
23770168
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biophysics
|
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| Research Institution | Osaka University (2012-2013)
Hokkaido University (2011) |
|---|
Principal Investigator |
ARAI Yoshiyuki 大阪大学, 産業科学研究所, 助教 (20444515)
|
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| Project Period (FY) |
2011 – 2012
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | １分子 / 金属増強効果 / 金属ナノ粒子 / １分子蛍光観察 / 1分子蛍光観察 / 金属増強 / 蛍光タンパク質 / ImageJ / １分子観察 |
|---|
| Research Abstract |
Single molecule imaging is a powerful technique for the direct observation of dynamics of biomolecules. However, photo-bleaching and signal fluctuations of fluorophores prevent long time observation. To overcome this problem, here, we made stable single molecule observation system by using nano-particle metal enhancement effect for fluorescent probes. We could observe signal enhancement of single molecule fluorescence by Ag nano-particles with Au as core. In addition, by coating SiO2 on Ag nano-particles coated glass surface, we could maintain the mammalian cell state and could observe metal enhanced fluorescent signals of fluorescently labeled epidermal growth factor.
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