Analysis of molecular function on DNA methylation regulator PGC7

• Project/Area Number: 23770224
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Cell biology
• Research Institution: Nagahama Institute of Bio-Science and Technology
• Principal Investigator: NAKAMURA Toshinobu 長浜バイオ大学, バイオサイエンス学部, 講師 (80403202)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: クロマチン / 受精卵 / DNA メチル化 / ヒストンメチル化 / DNAメチル化 / DNA / ヒストン / 脱メチル化 / メチル化 / PGC7

Research Abstract

In this study, we found that 5-hydroxymethylcytosine (5hmC) accumulated in the paternal genome along the rapid disappearance of 5-methylcytosine (5mC). In addition, we revealed that PGC7 binds di-methylated histone H3K9 to protect against conversion of 5mC to 5hmC in maternal genome.

Research Products

• [Journal Article] Effects of Dppa3 on DNA methylation dynamics during primordial germ cell development (2013)
  • Author(s): Nakashima H, Kimura T, Kaga Y. Nakatani T, Seki Y, Nakamura T, Nakano T
  • Journal Title: Biol Rep Volume: 88 Issue: 5 Pages: 1-9
  • DOI: 10.1095/biolreprod.112.105932
  • Peer Reviewed
• [Journal Article] GPAT2, a Mitochondrial Outer Membrane Protein in piRNA Biogenesis in Germline Stem Cells (2013)
  • Author(s): Shiromoto Y, Kuramochi-Miyagawa S, Daiba A, Chuma S, Katanaya A, Nishimura K, Ohtaka M, Nakanishi M, Nakamura T, Yoshinaga K, Asada N, Nakamura S, Yasunaga T, Kojima-Kita K, Itou D, Kimura T, Nakano T
  • Journal Title: RNA Volume: 19 Issue: 6 Pages: 803-810
  • DOI: 10.1261/rna.038521.113
  • Peer Reviewed
• [Journal Article] PGC7はヒストンH3K9me2との結合を介して受精卵における5mCから5hmCへの変換を阻害する (2012)
  • Author(s): 中村肇伸
  • Journal Title: 細胞工学 Volume: 31 Pages: 1038-1039
• [Journal Article] 母性因子PGC7はヒストンH3の9番目のリジン残基のジメチル化との結合を介し受精卵における5-メチルシトシンから5-ヒドロキシルメチルシトシンへの変換を阻害する (2012)
  • Author(s): 中村肇伸
  • Journal Title: ライフサイエンス新着論文レビュー
  • URL: http://first.lifesciencedb.jp/archives/5093
• [Journal Article] PGC7 binds histone H3K9me2 to protect against conversion of 5MeC to 5HmC in early embryos (2012)
  • Author(s): Nakamura, T., Liu, Y.U., Nakashima, H., Umehara, H., Inoue, K., Matoba, S., Tachibana, M., Ogura, A., Shinkai, Y., Nakano, T
  • Journal Title: Nature Volume: 486 Issue: 7403 Pages: 415-419
  • DOI: 10.1038/nature11093
  • Peer Reviewed
• [Journal Article] Essential role of DPPA3 for chromatin condensation in mouse oocytogenesis (2012)
  • Author(s): Liu YJ, Nakamura T, Nakano T
  • Journal Title: Biol Rep Volume: 86 Issue: 2 Pages: 1-8
  • DOI: 10.1095/biolreprod.111.095018
  • Peer Reviewed
• [Journal Article] 母性因子PGC7はヒストンH3の9番目のリジン残基のジメチル化との結合を介し受精卵における5-メチルシトシンから5-ヒドロキシルメチルシトシンへの変換を阻害する (2012)
  • Author(s): 中村 肇伸
  • Journal Title: ライフサイエンス新着論文レビュー Volume: 3 Pages: 5093-5093
• [Journal Article] RNAi-mediated knockdown of Xist can rescue the impaired postimplantation development of cloned mouse embryos (2011)
  • Author(s): Matoba S, et al
  • Journal Title: Proc Natl Acad Sci USA Volume: 108 Issue: 51 Pages: 20621-20626
  • DOI: 10.1073/pnas.1112664108
  • Peer Reviewed
• [Presentation] マウス受精卵における5mCから5hmCへの変換の制御機構 (2013)
  • Author(s): 中谷庸寿、木村透、小田昌朗、中島啓行、中村肇伸、仲野徹
  • Organizer: 第7回日本エピジェネティクス研究会年会
  • Place of Presentation: 奈良
  • Year and Date: 2013-05-31
• [Presentation] エピジェネティクスな変化と癌‐ゲノムの低メチル化が細胞の腫瘍化に及ぼす影響‐ (2013)
  • Author(s): 舟木壮一郎、中村肇伸、奥村明之進、仲野徹
  • Organizer: 第113回日本外科学会定期学術集会
  • Place of Presentation: 福岡
  • Year and Date: 2013-04-11
• [Presentation] Expression and purification for protein crystallography of a DNA methylation regulatory protein (2013)
  • Author(s): Nakamura T, Imadome G, Nakano T, Nakamura T, Yamagata Y
  • Organizer: International Symposium on Target recognition and expression mechanism of intrinsically disordered protein
  • Place of Presentation: 横浜
  • Year and Date: 2013-01-23
• [Presentation] Expression and purification for protein crystallography of a DNA methylation regulatory protein (2013)
  • Author(s): Nakamura T
  • Organizer: International Symposium on Target recognition and expression mechanism of intrinsically disordered protein
  • Place of Presentation: Yokohama
• [Presentation] エピジェネティクスな変化と癌 ‐ゲノムの低メチル化が細胞の腫瘍化に及ぼす影響‐ (2013)
  • Author(s): 舟木 壮一郎、中村 肇伸、奥村 明之進、仲野 徹
  • Organizer: 第113回日本外科学会定期学術集会
  • Place of Presentation: 福岡
• [Presentation] ゲノムワイドなDNA低メチル化と発癌との関連 (2012)
  • Author(s): 舟木壮一郎、中村肇伸、中島啓行、奥村明之進、仲野徹
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-11
• [Presentation] 受精卵における能動的メチル化消去の制御機構の解明、生殖系列の世代サイクルとエピゲノムネットワーク (2012)
  • Author(s): 中村肇伸
  • Organizer: 第5回公開シンポジウム
  • Place of Presentation: 京都(招待講演)
  • Year and Date: 2012-11-21
• [Presentation] メチル化制御因子PGC7の機演) (2012)
  • Author(s): 中村肇伸
  • Organizer: 第28回配偶子制御セミナー
  • Place of Presentation: 横浜(招待講演)
  • Year and Date: 2012-11-13
• [Presentation] Regulation of mouse brain development by DNA and histone methylation: From molecular basis to cognitive, behavioral, and motor abnormalities in gene-manipulated model mice (2012)
  • Author(s): Taga T, Kagawa T, Bizen N, Yamaguchi Y, Kokubu Y, Hattori S, Takao K, Miyakawa T, Inazawa J, Nakamura T, Nakano T
  • Organizer: The 35th Annual Meeting of the Japan Neuroscience Society, Symposium onEpigenetics and neuropsychiatric disorders
  • Place of Presentation: 名古屋
  • Year and Date: 2012-09-19
• [Presentation] PGC7/Stella binds to histone H3K9me2 to protect against conversion of 5mC to 5hmC in early embryos (2012)
  • Author(s): Nakamura T, Nakano T
  • Organizer: Cold Spring Harbor Laboratory meeting EPIGENETICS & CHROMATIN
  • Place of Presentation: 米国
  • Year and Date: 2012-09-13
• [Presentation] 受精卵における能動的DNA脱メチル化の制御機構の解明 (2012)
  • Author(s): 中村肇伸
  • Organizer: 生殖サイクル若手勉強会2012
  • Place of Presentation: 仙台(招待講演)
  • Year and Date: 2012-07-25
• [Presentation] DNA/ヒストンのメチル化制御による中枢神経系の発生制御:分子機構からヒト精神運動異常様行動を示す遺伝子変異モデルマウスまで (2012)
  • Author(s): 田賀哲也、鹿川哲史、備前典久、山口雄平、国分康博、服部聡子、高雄啓三、宮川剛、稲澤譲治、中村肇伸、仲野徹
  • Organizer: 第33回日本炎症・再生医学会
  • Place of Presentation: 福岡
  • Year and Date: 2012-07-15
• [Presentation] Epigenetic regulation of neural stem cell differentiation and brain development (2012)
  • Author(s): Taga T, Kagawa T, Bizen N, Yamaguchi Y, Kokubu Y, Hattori S, Takao K, Miyakawa T, Inazawa J, Nakamura T, Nakano T
  • Organizer: ISSCR 10th Annual Meeting
  • Place of Presentation: 淡路
  • Year and Date: 2012-06-01
• [Presentation] 5-methylcytosine hydroxylase TET3-mediated acquisition of astrocytic competence of midgestational neural stem cells (2012)
  • Author(s): Bizen N, Kagawa T, Nakamura T, Nakano T, Taga T
  • Organizer: 10th Stem Cell Research Symposium
  • Place of Presentation: 淡路
  • Year and Date: 2012-06-01
• [Presentation] 受精卵における能動的DNA脱メチル化の制御機構の解明 (2012)
  • Author(s): 中村肇伸
  • Organizer: 第6回日本エピジェネティクス研究会年会
  • Place of Presentation: 東京(招待講演)
  • Year and Date: 2012-05-15
• [Presentation] Global DNA hypomethylation and transformation (2012)
  • Author(s): Toshinobu Nakamura, Soichiro Funaki and Toru Nakano
  • Organizer: The 15th US-Japan Cellular and Gene Therapy Conference on Epigenetics in Cancer(招待講演)
  • Place of Presentation: 米国
  • Year and Date: 2012-02-23
• [Presentation] 受精卵における能動的DNA脱メチル化の制御機構の解明 (2012)
  • Author(s): 中村 肇伸
  • Organizer: 第6回日本エピジェネティクス研究会年会
  • Place of Presentation: 東京
  • Invited
• [Presentation] 5-methylcytosine hydroxylase TET3-mediated acquisition of astrocytic competence of mid-gestational neural stem cells (2012)
  • Author(s): Bizen N, Kagawa T, Nakamura T, Nakano T, Taga T
  • Organizer: 10th Stem Cell Research Symposium
  • Place of Presentation: Awaji
• [Presentation] Epigenetic regulation of neural stem cell differentiation and brain development (2012)
  • Author(s): Taga T, Kagawa T, Bizen N, Yamaguchi Y, Kokubu Y, Hattori S, Takao K, Miyakawa T, Inazawa J, Nakamura T, Nakano T
  • Organizer: ISSCR 10th Annual Meeting
  • Place of Presentation: Yokohama
• [Presentation] DNA／ヒストンのメチル化制御による中枢神経系の発生制御：分子機構からヒト精神運動異常様行動を示す遺伝子変異モデルマウスまで (2012)
  • Author(s): 田賀 哲也、鹿川 哲史、備前 典久、山口 雄平、国分 康博、服部 聡子、高雄 啓三、宮川 剛、稲澤 譲治、中村 肇伸、仲野 徹
  • Organizer: 第33回日本炎症・再生医学会
  • Place of Presentation: 福岡
• [Presentation] 受精卵における能動的DNA脱メチル化の制御機構の解明 (2012)
  • Author(s): 中村 肇伸
  • Organizer: 生殖サイクル若手勉強会2012
  • Place of Presentation: 仙台
  • Invited
• [Presentation] PGC7/Stella binds to histone H3K9me2 to protect against conversion of 5mC to 5hmC in early embryos (2012)
  • Author(s): Nakamura T, Nakano T
  • Organizer: Cold Spring Harbor Laboratory meeting EPIGENETICS & CHROMATIN
  • Place of Presentation: USA
• [Presentation] Regulation of mouse brain development by DNA and histone methylation: From molecular basis to cognitive, behavioral, and motor abnormalities in gene-manipulated model mice (2012)
  • Author(s): Taga T, Kagawa T, Bizen N, Yamaguchi Y, Kokubu Y, Hattori S, Takao K, Miyakawa T, Inazawa J, Nakamura T, Nakano T
  • Organizer: The 35th Annual Meeting of the Japan Neuroscience Society, Symposium on Epigenetics and neuropsychiatric disorders
  • Place of Presentation: Nagoya
• [Presentation] 受精卵における能動的メチル化消去の制御機構の解明 (2012)
  • Author(s): 中村 肇伸
  • Organizer: 「生殖系列の世代サイクルとエピゲノムネットワーク」第5回公開シンポジウム
  • Place of Presentation: 京都
  • Invited
• [Presentation] Global DNA hypomethylation and transformation. (2012)
  • Author(s): Toshinobu Nakamura, Soichiro Funaki and Toru Nakano
  • Organizer: The 15th US-Japan Cellular and Gene Therapy Conference on Epigenetics in Cancer(招待講演)
  • Place of Presentation: 米国、メリーランド
• [Presentation] Relationship between DNA modification and histone modification in early embryos (2011)
  • Author(s): Toru Nakano and Toshinobu Nakamura
  • Organizer: The 34th Annual Meeting of the Molecular Biology Society of Japan(招待講演)
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-16
• [Presentation] 受精卵における5MeCから5HmCへの変換制御機構の解明、文部科学省科学研究費補助金特定領域研究「生殖系列の世代サイクルとエピゲノムネットワーク」 (2011)
  • Author(s): 中村肇伸、Mark Wossidlo、立花誠、眞貝洋一、JoernWalter、仲野徹
  • Organizer: 第4回公開シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2011-11-18
• [Presentation] Joern Walter and Toru Nakano, Reciprocal regulation of DNA methylation and hydroxymethylation in early embryos by histone modification (2011)
  • Author(s): Toshinobu Nakamura, Mark Wossidlo, Makoto, Tachibana, Yoichi Shinkai
  • Organizer: The 84th Annual Meeting of the Japanese Biochemical Society(招待講演)
  • Place of Presentation: 京都
  • Year and Date: 2011-09-22
• [Presentation] 受精卵における5-メチルシトシンから5-ヒドロキシメチルシトシンへの変換 (2011)
  • Author(s): 中村肇伸
  • Organizer: 生殖サイクル若手勉強会2011
  • Place of Presentation: 大阪
  • Year and Date: 2011-07-13
• [Presentation] 受精卵における5-メチルシトシンから5-ヒドロキシメチルシトシンへの変換 (2011)
  • Author(s): 中村 肇伸、Mark Wossidlo、立花 誠、眞貝 洋一、Jörn Walter 、仲野 徹
  • Organizer: 第5回日本エピジェネティクス研究会年会
  • Place of Presentation: 熊本
• [Presentation] Reciprocal regulation of DNA methylation and hydroxymethylation in early embryos by histone modification (2011)
  • Author(s): Toshinobu Nakamura, Mark Wossidlo, Makoto Tachibana, Yoichi Shinkai, Jörn Walter and Toru Nakano
  • Organizer: The 84th Annual Meeting of the Japanese Biochemical Society(招待講演)
  • Place of Presentation: 京都
• [Presentation] 受精卵における5MeCから5HmCへの変換制御機構の解明 (2011)
  • Author(s): 中村 肇伸、Mark Wossidlo、立花 誠、眞貝 洋一、Jörn Walter 、仲野 徹
  • Organizer: 文部科学省科学研究費補助金 特定領域研究「生殖系列の世代サイクルとエピゲノムネットワーク」第４回公開シンポジウム
  • Place of Presentation: 大阪
• [Presentation] Relationship between DNA modification and histone modification in early embryos. (2011)
  • Author(s): Toru Nakano and Toshinobu Nakamura
  • Organizer: The 34th Annual Meeting of the Molecular Biology Society of Japan(招待講演)
  • Place of Presentation: 横浜
• [Presentation] メチル化制御因子PGC7の機能解析
  • Author(s): 中村 肇伸
  • Organizer: 第28回 配偶子制御セミナー
  • Place of Presentation: 横浜
  • Invited
• [Remarks] 中村肇伸講師がエピジェネティクス研究会奨励賞を受賞
  • URL: http://www.nagahama-i-bio.ac.jp/news/news/2012/05/post_185.html
• [Remarks] 哺乳類初期発生における「エピジェネティック制御」の謎を解明!
  • URL: http://www.osaka-u.ac.jp/ja/news/ResearchRelease/2012/06/20120604_1
• [Remarks] 中村肇伸講師らの共同研究が『ネイチャー』に掲載されました
  • URL: http://www.nagahama-i-bio.ac.jp/news/news/2012/06/post_190.html
• [Remarks] 中村肇伸講師が文部科学大臣表彰若手科学者賞を受賞しました
  • URL: http://www.nagahama-i-bio.ac.jp/news/news/2013/04/post_233.html
• [Remarks] たった１つの受精卵から細胞が分化する神秘の解明に大きな貢献
  • URL: http://www.nagahama-i-bio.ac.jp/news/news/2012/06/post_190.html
• [Remarks] 中村肇伸講師がエピジェネティクス研究会奨励賞を受賞
  • URL: http://www.nagahama-i-bio.ac.jp/news/news/2012/05/post_185.html
• [Remarks] 中村肇伸講師が文部科学大臣表彰若手科学者賞を受賞しました
  • URL: http://www.nagahama-i-bio.ac.jp/news/news/2013/04/post_233.html
• [Remarks]
  • URL: http://www.nagahama-i-bio.ac.jp/guide/kyoin/detail/post-6.html