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Analysis of molecular function on DNA methylation regulator PGC7

Research Project

Project/Area Number 23770224
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Cell biology
Research InstitutionNagahama Institute of Bio-Science and Technology

Principal Investigator

NAKAMURA Toshinobu  長浜バイオ大学, バイオサイエンス学部, 講師 (80403202)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywordsクロマチン / 受精卵 / DNA メチル化 / ヒストンメチル化 / DNAメチル化 / DNA / ヒストン / 脱メチル化 / メチル化 / PGC7
Research Abstract

In this study, we found that 5-hydroxymethylcytosine (5hmC) accumulated in the paternal genome along the rapid disappearance of 5-methylcytosine (5mC). In addition, we revealed that PGC7 binds di-methylated histone H3K9 to protect against conversion of 5mC to 5hmC in maternal genome.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (50 results)

All 2013 2012 2011 Other

All Journal Article (8 results) (of which Peer Reviewed: 5 results) Presentation (34 results) (of which Invited: 4 results) Remarks (8 results)

  • [Journal Article] Effects of Dppa3 on DNA methylation dynamics during primordial germ cell development2013

    • Author(s)
      Nakashima H, Kimura T, Kaga Y. Nakatani T, Seki Y, Nakamura T, Nakano T
    • Journal Title

      Biol Rep

      Volume: 88 Issue: 5 Pages: 1-9

    • DOI

      10.1095/biolreprod.112.105932

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] GPAT2, a Mitochondrial Outer Membrane Protein in piRNA Biogenesis in Germline Stem Cells2013

    • Author(s)
      Shiromoto Y, Kuramochi-Miyagawa S, Daiba A, Chuma S, Katanaya A, Nishimura K, Ohtaka M, Nakanishi M, Nakamura T, Yoshinaga K, Asada N, Nakamura S, Yasunaga T, Kojima-Kita K, Itou D, Kimura T, Nakano T
    • Journal Title

      RNA

      Volume: 19 Issue: 6 Pages: 803-810

    • DOI

      10.1261/rna.038521.113

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] PGC7はヒストンH3K9me2との結合を介して受精卵における5mCから5hmCへの変換を阻害する2012

    • Author(s)
      中村肇伸
    • Journal Title

      細胞工学

      Volume: 31 Pages: 1038-1039

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Journal Article] 母性因子PGC7はヒストンH3の9番目のリジン残基のジメチル化との結合を介し受精卵における5-メチルシトシンから5-ヒドロキシルメチルシトシンへの変換を阻害する2012

    • Author(s)
      中村肇伸
    • Journal Title

      ライフサイエンス新着論文レビュー

    • URL

      http://first.lifesciencedb.jp/archives/5093

    • Related Report
      2012 Final Research Report
  • [Journal Article] PGC7 binds histone H3K9me2 to protect against conversion of 5MeC to 5HmC in early embryos2012

    • Author(s)
      Nakamura, T., Liu, Y.U., Nakashima, H., Umehara, H., Inoue, K., Matoba, S., Tachibana, M., Ogura, A., Shinkai, Y., Nakano, T
    • Journal Title

      Nature

      Volume: 486 Issue: 7403 Pages: 415-419

    • DOI

      10.1038/nature11093

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Essential role of DPPA3 for chromatin condensation in mouse oocytogenesis2012

    • Author(s)
      Liu YJ, Nakamura T, Nakano T
    • Journal Title

      Biol Rep

      Volume: 86 Issue: 2 Pages: 1-8

    • DOI

      10.1095/biolreprod.111.095018

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] 母性因子PGC7はヒストンH3の9番目のリジン残基のジメチル化との結合を介し受精卵における5-メチルシトシンから5-ヒドロキシルメチルシトシンへの変換を阻害する2012

    • Author(s)
      中村 肇伸
    • Journal Title

      ライフサイエンス新着論文レビュー

      Volume: 3 Pages: 5093-5093

    • Related Report
      2012 Annual Research Report
  • [Journal Article] RNAi-mediated knockdown of Xist can rescue the impaired postimplantation development of cloned mouse embryos2011

    • Author(s)
      Matoba S, et al
    • Journal Title

      Proc Natl Acad Sci USA

      Volume: 108 Issue: 51 Pages: 20621-20626

    • DOI

      10.1073/pnas.1112664108

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Presentation] マウス受精卵における5mCから5hmCへの変換の制御機構2013

    • Author(s)
      中谷庸寿、木村透、小田昌朗、中島啓行、中村肇伸、仲野徹
    • Organizer
      第7回日本エピジェネティクス研究会年会
    • Place of Presentation
      奈良
    • Year and Date
      2013-05-31
    • Related Report
      2012 Final Research Report
  • [Presentation] エピジェネティクスな変化と癌‐ゲノムの低メチル化が細胞の腫瘍化に及ぼす影響‐2013

    • Author(s)
      舟木壮一郎、中村肇伸、奥村明之進、仲野徹
    • Organizer
      第113回日本外科学会定期学術集会
    • Place of Presentation
      福岡
    • Year and Date
      2013-04-11
    • Related Report
      2012 Final Research Report
  • [Presentation] Expression and purification for protein crystallography of a DNA methylation regulatory protein2013

    • Author(s)
      Nakamura T, Imadome G, Nakano T, Nakamura T, Yamagata Y
    • Organizer
      International Symposium on Target recognition and expression mechanism of intrinsically disordered protein
    • Place of Presentation
      横浜
    • Year and Date
      2013-01-23
    • Related Report
      2012 Final Research Report
  • [Presentation] Expression and purification for protein crystallography of a DNA methylation regulatory protein2013

    • Author(s)
      Nakamura T
    • Organizer
      International Symposium on Target recognition and expression mechanism of intrinsically disordered protein
    • Place of Presentation
      Yokohama
    • Related Report
      2012 Annual Research Report
  • [Presentation] エピジェネティクスな変化と癌 ‐ゲノムの低メチル化が細胞の腫瘍化に及ぼす影響‐2013

    • Author(s)
      舟木 壮一郎、中村 肇伸、奥村 明之進、仲野 徹
    • Organizer
      第113回日本外科学会定期学術集会
    • Place of Presentation
      福岡
    • Related Report
      2012 Annual Research Report
  • [Presentation] ゲノムワイドなDNA低メチル化と発癌との関連2012

    • Author(s)
      舟木壮一郎、中村肇伸、中島啓行、奥村明之進、仲野徹
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Year and Date
      2012-12-11
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] 受精卵における能動的メチル化消去の制御機構の解明、生殖系列の世代サイクルとエピゲノムネットワーク2012

    • Author(s)
      中村肇伸
    • Organizer
      第5回公開シンポジウム
    • Place of Presentation
      京都(招待講演)
    • Year and Date
      2012-11-21
    • Related Report
      2012 Final Research Report
  • [Presentation] メチル化制御因子PGC7の機演)2012

    • Author(s)
      中村肇伸
    • Organizer
      第28回配偶子制御セミナー
    • Place of Presentation
      横浜(招待講演)
    • Year and Date
      2012-11-13
    • Related Report
      2012 Final Research Report
  • [Presentation] Regulation of mouse brain development by DNA and histone methylation: From molecular basis to cognitive, behavioral, and motor abnormalities in gene-manipulated model mice2012

    • Author(s)
      Taga T, Kagawa T, Bizen N, Yamaguchi Y, Kokubu Y, Hattori S, Takao K, Miyakawa T, Inazawa J, Nakamura T, Nakano T
    • Organizer
      The 35th Annual Meeting of the Japan Neuroscience Society, Symposium onEpigenetics and neuropsychiatric disorders
    • Place of Presentation
      名古屋
    • Year and Date
      2012-09-19
    • Related Report
      2012 Final Research Report
  • [Presentation] PGC7/Stella binds to histone H3K9me2 to protect against conversion of 5mC to 5hmC in early embryos2012

    • Author(s)
      Nakamura T, Nakano T
    • Organizer
      Cold Spring Harbor Laboratory meeting EPIGENETICS & CHROMATIN
    • Place of Presentation
      米国
    • Year and Date
      2012-09-13
    • Related Report
      2012 Final Research Report
  • [Presentation] 受精卵における能動的DNA脱メチル化の制御機構の解明2012

    • Author(s)
      中村肇伸
    • Organizer
      生殖サイクル若手勉強会2012
    • Place of Presentation
      仙台(招待講演)
    • Year and Date
      2012-07-25
    • Related Report
      2012 Final Research Report
  • [Presentation] DNA/ヒストンのメチル化制御による中枢神経系の発生制御:分子機構からヒト精神運動異常様行動を示す遺伝子変異モデルマウスまで2012

    • Author(s)
      田賀哲也、鹿川哲史、備前典久、山口雄平、国分康博、服部聡子、高雄啓三、宮川剛、稲澤譲治、中村肇伸、仲野徹
    • Organizer
      第33回日本炎症・再生医学会
    • Place of Presentation
      福岡
    • Year and Date
      2012-07-15
    • Related Report
      2012 Final Research Report
  • [Presentation] Epigenetic regulation of neural stem cell differentiation and brain development2012

    • Author(s)
      Taga T, Kagawa T, Bizen N, Yamaguchi Y, Kokubu Y, Hattori S, Takao K, Miyakawa T, Inazawa J, Nakamura T, Nakano T
    • Organizer
      ISSCR 10th Annual Meeting
    • Place of Presentation
      淡路
    • Year and Date
      2012-06-01
    • Related Report
      2012 Final Research Report
  • [Presentation] 5-methylcytosine hydroxylase TET3-mediated acquisition of astrocytic competence of midgestational neural stem cells2012

    • Author(s)
      Bizen N, Kagawa T, Nakamura T, Nakano T, Taga T
    • Organizer
      10th Stem Cell Research Symposium
    • Place of Presentation
      淡路
    • Year and Date
      2012-06-01
    • Related Report
      2012 Final Research Report
  • [Presentation] 受精卵における能動的DNA脱メチル化の制御機構の解明2012

    • Author(s)
      中村肇伸
    • Organizer
      第6回日本エピジェネティクス研究会年会
    • Place of Presentation
      東京(招待講演)
    • Year and Date
      2012-05-15
    • Related Report
      2012 Final Research Report
  • [Presentation] Global DNA hypomethylation and transformation2012

    • Author(s)
      Toshinobu Nakamura, Soichiro Funaki and Toru Nakano
    • Organizer
      The 15th US-Japan Cellular and Gene Therapy Conference on Epigenetics in Cancer(招待講演)
    • Place of Presentation
      米国
    • Year and Date
      2012-02-23
    • Related Report
      2012 Final Research Report
  • [Presentation] 受精卵における能動的DNA脱メチル化の制御機構の解明2012

    • Author(s)
      中村 肇伸
    • Organizer
      第6回日本エピジェネティクス研究会年会
    • Place of Presentation
      東京
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 5-methylcytosine hydroxylase TET3-mediated acquisition of astrocytic competence of mid-gestational neural stem cells2012

    • Author(s)
      Bizen N, Kagawa T, Nakamura T, Nakano T, Taga T
    • Organizer
      10th Stem Cell Research Symposium
    • Place of Presentation
      Awaji
    • Related Report
      2012 Annual Research Report
  • [Presentation] Epigenetic regulation of neural stem cell differentiation and brain development2012

    • Author(s)
      Taga T, Kagawa T, Bizen N, Yamaguchi Y, Kokubu Y, Hattori S, Takao K, Miyakawa T, Inazawa J, Nakamura T, Nakano T
    • Organizer
      ISSCR 10th Annual Meeting
    • Place of Presentation
      Yokohama
    • Related Report
      2012 Annual Research Report
  • [Presentation] DNA/ヒストンのメチル化制御による中枢神経系の発生制御:分子機構からヒト精神運動異常様行動を示す遺伝子変異モデルマウスまで2012

    • Author(s)
      田賀 哲也、鹿川 哲史、備前 典久、山口 雄平、国分 康博、服部 聡子、高雄 啓三、宮川 剛、稲澤 譲治、中村 肇伸、仲野 徹
    • Organizer
      第33回日本炎症・再生医学会
    • Place of Presentation
      福岡
    • Related Report
      2012 Annual Research Report
  • [Presentation] 受精卵における能動的DNA脱メチル化の制御機構の解明2012

    • Author(s)
      中村 肇伸
    • Organizer
      生殖サイクル若手勉強会2012
    • Place of Presentation
      仙台
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] PGC7/Stella binds to histone H3K9me2 to protect against conversion of 5mC to 5hmC in early embryos2012

    • Author(s)
      Nakamura T, Nakano T
    • Organizer
      Cold Spring Harbor Laboratory meeting EPIGENETICS & CHROMATIN
    • Place of Presentation
      USA
    • Related Report
      2012 Annual Research Report
  • [Presentation] Regulation of mouse brain development by DNA and histone methylation: From molecular basis to cognitive, behavioral, and motor abnormalities in gene-manipulated model mice2012

    • Author(s)
      Taga T, Kagawa T, Bizen N, Yamaguchi Y, Kokubu Y, Hattori S, Takao K, Miyakawa T, Inazawa J, Nakamura T, Nakano T
    • Organizer
      The 35th Annual Meeting of the Japan Neuroscience Society, Symposium on Epigenetics and neuropsychiatric disorders
    • Place of Presentation
      Nagoya
    • Related Report
      2012 Annual Research Report
  • [Presentation] 受精卵における能動的メチル化消去の制御機構の解明2012

    • Author(s)
      中村 肇伸
    • Organizer
      「生殖系列の世代サイクルとエピゲノムネットワーク」第5回公開シンポジウム
    • Place of Presentation
      京都
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Global DNA hypomethylation and transformation.2012

    • Author(s)
      Toshinobu Nakamura, Soichiro Funaki and Toru Nakano
    • Organizer
      The 15th US-Japan Cellular and Gene Therapy Conference on Epigenetics in Cancer(招待講演)
    • Place of Presentation
      米国、メリーランド
    • Related Report
      2011 Research-status Report
  • [Presentation] Relationship between DNA modification and histone modification in early embryos2011

    • Author(s)
      Toru Nakano and Toshinobu Nakamura
    • Organizer
      The 34th Annual Meeting of the Molecular Biology Society of Japan(招待講演)
    • Place of Presentation
      横浜
    • Year and Date
      2011-12-16
    • Related Report
      2012 Final Research Report
  • [Presentation] 受精卵における5MeCから5HmCへの変換制御機構の解明、文部科学省科学研究費補助金特定領域研究「生殖系列の世代サイクルとエピゲノムネットワーク」2011

    • Author(s)
      中村肇伸、Mark Wossidlo、立花誠、眞貝洋一、JoernWalter、仲野徹
    • Organizer
      第4回公開シンポジウム
    • Place of Presentation
      大阪
    • Year and Date
      2011-11-18
    • Related Report
      2012 Final Research Report
  • [Presentation] Joern Walter and Toru Nakano, Reciprocal regulation of DNA methylation and hydroxymethylation in early embryos by histone modification2011

    • Author(s)
      Toshinobu Nakamura, Mark Wossidlo, Makoto, Tachibana, Yoichi Shinkai
    • Organizer
      The 84th Annual Meeting of the Japanese Biochemical Society(招待講演)
    • Place of Presentation
      京都
    • Year and Date
      2011-09-22
    • Related Report
      2012 Final Research Report
  • [Presentation] 受精卵における5-メチルシトシンから5-ヒドロキシメチルシトシンへの変換2011

    • Author(s)
      中村肇伸
    • Organizer
      生殖サイクル若手勉強会2011
    • Place of Presentation
      大阪
    • Year and Date
      2011-07-13
    • Related Report
      2012 Final Research Report 2011 Research-status Report
  • [Presentation] 受精卵における5-メチルシトシンから5-ヒドロキシメチルシトシンへの変換2011

    • Author(s)
      中村 肇伸、Mark Wossidlo、立花 誠、眞貝 洋一、Jörn Walter 、仲野 徹
    • Organizer
      第5回日本エピジェネティクス研究会年会
    • Place of Presentation
      熊本
    • Related Report
      2011 Research-status Report
  • [Presentation] Reciprocal regulation of DNA methylation and hydroxymethylation in early embryos by histone modification2011

    • Author(s)
      Toshinobu Nakamura, Mark Wossidlo, Makoto Tachibana, Yoichi Shinkai, Jörn Walter and Toru Nakano
    • Organizer
      The 84th Annual Meeting of the Japanese Biochemical Society(招待講演)
    • Place of Presentation
      京都
    • Related Report
      2011 Research-status Report
  • [Presentation] 受精卵における5MeCから5HmCへの変換制御機構の解明2011

    • Author(s)
      中村 肇伸、Mark Wossidlo、立花 誠、眞貝 洋一、Jörn Walter 、仲野 徹
    • Organizer
      文部科学省科学研究費補助金 特定領域研究「生殖系列の世代サイクルとエピゲノムネットワーク」第4回公開シンポジウム
    • Place of Presentation
      大阪
    • Related Report
      2011 Research-status Report
  • [Presentation] Relationship between DNA modification and histone modification in early embryos.2011

    • Author(s)
      Toru Nakano and Toshinobu Nakamura
    • Organizer
      The 34th Annual Meeting of the Molecular Biology Society of Japan(招待講演)
    • Place of Presentation
      横浜
    • Related Report
      2011 Research-status Report
  • [Presentation] メチル化制御因子PGC7の機能解析

    • Author(s)
      中村 肇伸
    • Organizer
      第28回 配偶子制御セミナー
    • Place of Presentation
      横浜
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Remarks] 中村肇伸講師がエピジェネティクス研究会奨励賞を受賞

    • URL

      http://www.nagahama-i-bio.ac.jp/news/news/2012/05/post_185.html

    • Related Report
      2012 Final Research Report
  • [Remarks] 哺乳類初期発生における「エピジェネティック制御」の謎を解明!

    • URL

      http://www.osaka-u.ac.jp/ja/news/ResearchRelease/2012/06/20120604_1

    • Related Report
      2012 Final Research Report
  • [Remarks] 中村肇伸講師らの共同研究が『ネイチャー』に掲載されました

    • URL

      http://www.nagahama-i-bio.ac.jp/news/news/2012/06/post_190.html

    • Related Report
      2012 Final Research Report
  • [Remarks] 中村肇伸講師が文部科学大臣表彰若手科学者賞を受賞しました

    • URL

      http://www.nagahama-i-bio.ac.jp/news/news/2013/04/post_233.html

    • Related Report
      2012 Final Research Report
  • [Remarks] たった1つの受精卵から細胞が分化する神秘の解明に大きな貢献

    • URL

      http://www.nagahama-i-bio.ac.jp/news/news/2012/06/post_190.html

    • Related Report
      2012 Annual Research Report
  • [Remarks] 中村肇伸講師がエピジェネティクス研究会奨励賞を受賞

    • URL

      http://www.nagahama-i-bio.ac.jp/news/news/2012/05/post_185.html

    • Related Report
      2012 Annual Research Report
  • [Remarks] 中村肇伸講師が文部科学大臣表彰若手科学者賞を受賞しました

    • URL

      http://www.nagahama-i-bio.ac.jp/news/news/2013/04/post_233.html

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.nagahama-i-bio.ac.jp/guide/kyoin/detail/post-6.html

    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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