Study of the molecular mechanism of oxidative stress response induced by mitochondrial ROS

• Project/Area Number: 23770227
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Cell biology
• Research Institution: Osaka University
• Principal Investigator: SHIBUYA Toshiharu 大阪大学, 医学系研究科, 助教 (70448033)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
• Keywords: 酸化ストレス / ミトコンドリア / 細胞内情報伝達 / 細胞死 / 線虫 / ヒト培養細胞 / 活性酸素

Research Abstract

An experimental assay system was employed to generate excessive ROS in the mitochondria in a precisely controlled spatio-temporal manner. Using mammalian cells and worm C. elegans generating mitochondrial ROS, I analyzed the stress response and the molecular mechanism induced by mitochondrial ROS in vitro and in vivo. I revealed that factors involved in the mitochondrial permeability transition (Cyclophilin D and Ca++) were contributed to the mitochondrial oxidative stress response and that mitochondrial ROS affected the development of C.elegans.

Research Products

• [Journal Article] Deleterious effects of mitochondrial ROS generated by KillerRed photodynamic action in human cell lines and C. elegans. (2012)
  • Author(s): Shibuya, T. & Tsujimoto, Y.
  • Journal Title: Journal of photochemistry and photobiology B: Biology Volume: 117C Pages: 1-12
• [Journal Article] Deleterious effects of mitochondrial ROS generated by KillerRed photodynamic action in human cell lines and C. elegans. (2012)
  • Author(s): Shibuya, T.
  • Journal Title: J. Photochem. Photobiol. Biol. Volume: 117 Pages: 1-12
  • DOI: 10.1016/j.jphotobiol.2012.08.005
  • Peer Reviewed
• [Presentation] Deleterious effects of mitochondrial ROS generated by KillerRed photodynamic action in human cell lines and C. elegans. (2012)
  • Author(s): 渋谷利治
  • Organizer: 第35回日本分子生物学会
  • Place of Presentation: 福岡国際会議場(福岡市、福岡県)
  • Year and Date: 2012-12-10
• [Presentation] Deleterious effects of mitochondrial ROS generated by KillerRed photodynamic action in human cell lines and C. elegans.
  • Author(s): Toshiharu Shibuya
  • Organizer: 第３５回 日本分子生物学会
  • Place of Presentation: 福岡
• [Remarks]
  • URL: http://www.med.osaka-u.ac.jp/pub/gene/www/laboratory/shibuya1.html
• [Remarks] 大阪大学医学系研究科遺伝子学
  • URL: http://www.med.osaka-u.ac.jp/pub/gene/www/index.html
• [Remarks]
  • URL: http://www.med.osaka-u.ac.jp/pub/gene/www/index.html