Analysis of melanosome transfer between melanocytes and keratinocytes

• Project/Area Number: 23770233
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Cell biology
• Research Institution: Sapporo Medical University
• Principal Investigator: HIDA Tokimasa 札幌医科大学, 医学部, 助教 (90464487)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: メラノソーム / 細胞生物学

Research Abstract

A new co-culture method for melanocytes and keratinocytes has been developed. By using this method, melanosomal transfer from melanocytes to keratinocytes could be visualized with electron and optical microscopes and the melanin content of transferred melanosomes could be analyzed. The effect of inhibitors of melanin synthesis on melanosomal transfer could also be analyzed. To apply the result for clinical practice, the melanin distribution in the nipple skin of patients with pigmented mammary Paget's disease was examined with histopathology, immunohistochemistry and dermoscopy. It was suggested that tumor cells transferred melanin from reactive melanocytes are subject to passive elimination from the epidermis and form characteristic dermoscopic patterns. This finding is useful in diagnosing pigmented mammary Paget's disease.

Research Products

• [Journal Article] Ankyloblepharon-ectodermal defects-cleft lip/palate syndrome : a case with a novel p63 mutation associated with abnormal keratohyalin granules (2014)
  • Author(s): Hida T, Kase K, Hamada T, Matsuda M, Hashimoto T, Yamashita T.
  • Journal Title: Eur J Dermatol Volume: (in press)
  • Peer Reviewed
• [Journal Article] Pigmented mammary Paget’s disease presenting with dermoscopic features of multiple dots. (2013)
  • Author(s): Hida T, Yamashita T
  • Journal Title: Australas J Dermatol Volume: in print Issue: 4 Pages: 260-262
  • DOI: 10.1111/ajd.12086
  • Peer Reviewed
• [Journal Article] Influence of isoflavone intake and equol-producing intestinal flora on prostate cancer risk (2013)
  • Author(s): Sugiyama Y, Masumori N, Fukuta F, Yoneta A, Hida T, Yamashita T, Minatoya M, Nagata Y, Mori M, Tsuji H, Akaza H, Tsukamoto T
  • Journal Title: Asian Pac J Cancer Prev Volume: 14 Issue: 1 Pages: 1-4
  • DOI: 10.7314/apjcp.2013.14.1.1
  • Peer Reviewed
• [Journal Article] Pigmented mammary Paget's disease mimicking melanoma : report of three cases (2012)
  • Author(s): Hida T, Yoneta A, Nishizaka T, Ohmura T, Suzuki Y, Kameshima H, Yamashita T
  • Journal Title: Eur J Dermatol Volume: 22 Issue: 1 Pages: 121-124
  • DOI: 10.1684/ejd.2011.1580
  • Peer Reviewed
• [Journal Article] Etanercept-induced lichen planus-like eruptions following the lines of Blaschko (2012)
  • Author(s): Utsu M, Hida T, Takahashi H, Yamashita T
  • Journal Title: Eur J Dermatol Volume: 22 Issue: 4 Pages: 544-545
  • DOI: 10.1684/ejd.2012.1747
  • Peer Reviewed
• [Presentation] 眼皮膚白皮症4型の一例 (2013)
  • Author(s): 肥田時征,黄倉真恵,山下利春
  • Organizer: 第25回日本色素細胞学会学術大会
  • Place of Presentation: 大阪
• [Presentation] Ankyloblepharon-ectodermal defects-cleft lip/palate syndrome (2013)
  • Author(s): 肥田時征,加瀬貴美,濱田尚宏,松田光弘,橋本隆,浅沼秀臣,重富浩子,山下利春
  • Organizer: 日本人類遺伝学会第58回大会
  • Place of Presentation: 仙台
• [Presentation] AEC症候群の1例 (2013)
  • Author(s): 肥田時征,加瀬貴美,濱田尚宏,松田光弘,橋本隆,浅沼秀臣,山下利春
  • Organizer: 第395回日本皮膚科学会北海道地方会
  • Place of Presentation: 札幌
• [Presentation] Dermoscopic findings of pigmented mammary Paget's disease (2013)
  • Author(s): Hida T, Yoneta A, Yamashita T
  • Organizer: The 9th Asian Dermatological Congress
  • Place of Presentation: Hong Kong
• [Presentation] 原因物質により多彩な臨床像を示す異物肉芽腫～診断の第一歩として問診が重要な肉芽腫～ (2013)
  • Author(s): 肥田時征
  • Organizer: 第112回日本皮膚科学会総会教育講演
  • Place of Presentation: 横浜
• [Presentation] Dermoscopic findings of pigmented mammary paget’s Disease. (2013)
  • Author(s): Hida T, Yoneta A, Yamashita T.
  • Organizer: 9th Asian Dermatological Congress
  • Place of Presentation: Hong Kong
• [Presentation] インドシアニングリーン蛍光法を併用した悪性黒色腫センチネルリンパ節生検 (2012)
  • Author(s): 肥田時征,筬井泰江,小野一郎,山下利春
  • Organizer: 第111回日本皮膚科学会総会
  • Place of Presentation: 京都
• [Presentation] Diagnosis of xeroderma pigmentosum by recombinant adenovirus infection and measurement of UV sensitivity of patient fibroblasts (2012)
  • Author(s): Okura M, Osai Y, Hida T, Mori S, Yamashita T
  • Organizer: The 2nd Eastern Asia Dermatology Congress
  • Place of Presentation: Beijing, China
• [Presentation] Effects of a low-molecular-weight polyphenol (oligonol) on the growth and melanogenesis of primary melanocytes and melanoma cells (2011)
  • Author(s): Okura M, Hagiwara K, Hida T, Yoneta A, Yanagisawa K, Horio Y, Yamashita T
  • Organizer: The 19th International Pigment Cell Conference
  • Place of Presentation: Bordeaux, France
• [Presentation] Two cases of advanced melanoma treated with dendritic cell vaccination therapy (2011)
  • Author(s): Yoneta A, Nishizaka T, Hida T, Goto S, Ozawa M, Yamashita T
  • Organizer: The 22nd World Congress of Dermatology
  • Place of Presentation: Seoul, Korea
• [Book] 貧血母斑.皮膚科臨床アセット15-母斑と母斑症- (2013)
  • Author(s): 肥田時征,山下利春
  • Publisher: 中山書店
• [Book] 皮膚科臨床アセット15 (2013)
  • Author(s): 肥田時征，山下利春
  • Total Pages: 4
  • Publisher: 中山書店