| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Research Abstract |
A new co-culture method for melanocytes and keratinocytes has been developed. By using this method, melanosomal transfer from melanocytes to keratinocytes could be visualized with electron and optical microscopes and the melanin content of transferred melanosomes could be analyzed. The effect of inhibitors of melanin synthesis on melanosomal transfer could also be analyzed. To apply the result for clinical practice, the melanin distribution in the nipple skin of patients with pigmented mammary Paget's disease was examined with histopathology, immunohistochemistry and dermoscopy. It was suggested that tumor cells transferred melanin from reactive melanocytes are subject to passive elimination from the epidermis and form characteristic dermoscopic patterns. This finding is useful in diagnosing pigmented mammary Paget's disease.
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